LOCUS       sequence-20645-e         657 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG425GPD-ccdB-EYFP
FEATURES             Location/Qualifiers
     source          1..657
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(126..250)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(327..348)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(588..607)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttcgtttcgg ggaagagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat
       61 taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg
      121 tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca
      181 aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt
      241 acaaagtggt gatgggctgc aggaattcga tatcaagctt aatggtgagc aagggcgagg
      301 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      361 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
      421 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct
      481 acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      541 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
      601 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagc
//
