LOCUS       sequence-206493-        4141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with yeast ATG18 (PLEKHA3). 
            Binds PtdIns(3,5P)2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-ATG18
FEATURES             Location/Qualifiers
     source          1..4141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(624..748)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2250..2345)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2382..2401)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2383..2401)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2406..2422)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2406..2422)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2524..2543)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2535..3344
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2602..2621)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3491..4079
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3980..3999
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac
      601 taataccatc taagtagttg gttcatagtg actgcatatg ttgtgtttta cagtattatg
      661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
      721 ctcgttcaac tttcttgtac aaagtggtgt ctgattcatc acctactatc aactttatta
      781 atttcaatca aaccggaacg tgtatttccc ttggaacgtc gaaaggtttc aaaatattca
      841 attgtgagcc cttcggaaaa ttttattcag aggacagtgg gggctatgct atcgtcgaga
      901 tgttgttctc cacctcgtta ctagccctcg ttgggatagg cgatcaacct gcgctttcac
      961 caaggagatt gcgtataatc aacacaaaaa aacattctat tatctgtgag gtgactttcc
     1021 ctacttctat tctgagtgtg aaaatgaata agtctcgatt ggtggtactt ttacaagagc
     1081 agatttatat ttatgatatc aacaccatga gactattgca tactatagaa acaaacccta
     1141 acccacgtgg ccttatggct atgtctcctt cggtagccaa cagctattta gtgtatccat
     1201 caccaccaaa agttattaac tccgaaatta aagctcatgc caccacaaac aatatcacat
     1261 tgtcagttgg tggcaacaca gagaccagtt tcaagagaga tcagcaagat gctggccata
     1321 gtgacattag cgacttggat cagtattcga gctttactaa gagggatgat gcggatccaa
     1381 caagcagtaa cggcggtaac agcagtataa taaagaatgg tgatgtgatc gtattcaact
     1441 tggaaacatt acagccaacc atggtcatcg aagctcataa gggcgagatt gctgcaatgg
     1501 caattagttt tgatgggaca ctaatggcta ccgcctctga taaaggtact atcatcaggg
     1561 tctttgacat tgaaacgggt gataagatct accaattcag gagagggacg tacgcgacaa
     1621 gaatttactc catatcattc agtgaagata gccagtactt ggcggttacc ggctcttcca
     1681 aaaccgtgca tatcttcaaa ttggggcatt caatgagcaa caataaacta gacagcgatg
     1741 atagcaacat ggaagaagct gcagccgatg attcatcgct cgataccacc agtatcgatg
     1801 cgctgagtga cgaggaaaac ccgacaagac tcgcaagaga accatatgtg gatgcatcaa
     1861 gaaagacaat gggtaggatg atacgttact cttctcaaaa gctatcccga agagctgcca
     1921 gaacattggg tcagattttc cccatcaaag ttacatcgtt gttggaatcc tcgcgccatt
     1981 ttgcgtcttt gaaacttccc gttgaaacca attcccatgt aatgaccata tcaagtatag
     2041 gctctccaat agatatagac acatccgagt atccggaact cttcgaaact ggcaattccg
     2101 caagtacaga gtcctaccat gagcctgtta tgaagatggt ccccatcagg gtcgtttcct
     2161 cggatggata cctatacaac tttgttatgg acccggagag aggcggcgat tgcttaatat
     2221 tgtcacagta ttccatcttg atggattgac aactttatta tacaaagttg gcattataaa
     2281 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     2341 tatttggagc tccatggtag cgttaacgcg gccgcgatat cccctatagt gagtcgtatt
     2401 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
     2461 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta
     2521 atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca
     2581 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
     2641 cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
     2701 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat
     2761 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
     2821 ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg
     2881 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta
     2941 attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata
     3001 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag
     3061 tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg
     3121 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
     3181 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
     3241 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
     3301 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt
     3361 ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa
     3421 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     3481 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3541 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3601 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     3661 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3721 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3781 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3841 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc
     3901 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     3961 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4021 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4081 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4141 t
//
