LOCUS       sequence-20659-e         641 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG304GPD-ccdB-HA
FEATURES             Location/Qualifiers
     source          1..641
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(122..246)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             287..376
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     promoter        complement(421..439)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(460..476)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(460..476)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(473..495)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    484..500
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(508..514,515..532,533..538))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    553..574
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 atcggggaag agtggctgat ctacagccac cgcgaaaatg acatcaaaaa cgccattaac
       61 ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga
      121 ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
      181 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa
      241 agtggtgatg ggctgcagga attcgatatc aagcttaggt ggaatgtacc catacgatgt
      301 tcctgactat gcgggctatc cctatgacgt cccggactat gcaggatcct atccatatga
      361 cgttccagat tacgctgctc agtgctaact cgaggggggg cccggtacca gcttttgttc
      421 cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
      481 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
      541 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
      601 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc g
//
