LOCUS       sequence-206629-        3367 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab33ba.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab33ba
FEATURES             Location/Qualifiers
     source          1..3367
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(624..748)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1476..1571)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1608..1627)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1609..1627)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1632..1648)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1632..1648)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1750..1769)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1761..2570
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1828..1847)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2717..3305
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3206..3225
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac
      601 taataccatc taagtagttg gttcatagtg actgcatatg ttgtgtttta cagtattatg
      661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
      721 ctcgttcaac tttcttgtac aaagtggtgg cagatatcga gtcctctttt gagttcagct
      781 cttctctgac cagctcgtca ctgcctcctc ctcggacacg catctttaaa atcatcgtga
      841 ttggagactc aggggttggc aagacttgcc tcacctatag attctgtgca ggcaagtttc
      901 cagacaagac tgaggccact attggggtgg atttccgaga gaaggttatt gagatagatg
      961 gcgagaaaat caaggtccag ttgtgggaca cggcaggcca ggagcgtttc cgtaagagca
     1021 tggtgcagca ctactaccgc aacgtccatg ctgtggtgtt tgtctatgac gtcaccaatg
     1081 ccgccagctt ccgaagcctc ccagcgtgga ttgaagagtg tcgccaacat gcactcggtc
     1141 aggaggtgcc aagaatcctt gtcggcaaca aatgcgacct acgtcacgcc gctcaagtaa
     1201 gcacagatgt tgcacagcag tttgcagata cacactcaat gcctcttttt gagacctcgg
     1261 ccaaaaaccc ctacgggaat gaagacggca ctcagaataa tagtgaccac gtggaggcca
     1321 tttttatgac agtggcgcac aagctgaaat cccaaaagcc gctggtttta agccagccac
     1381 cttgcggata tggagatact gtcactctta ggaggcaaga ccaggaggac gggggaaact
     1441 ggggctgcgg ttgttggaga agctaatgaa gtgcacaact ttattataca aagttggcat
     1501 tataaaaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
     1561 aatcattatt tggagctcca tggtagcgtt aacgcggccg cgatatcccc tatagtgagt
     1621 cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat
     1681 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa
     1741 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa
     1801 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
     1861 caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac
     1921 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
     1981 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt
     2041 tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt
     2101 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
     2161 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa
     2221 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
     2281 aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc
     2341 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
     2401 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
     2461 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
     2521 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg
     2581 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat
     2641 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa
     2701 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     2761 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2821 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     2881 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2941 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3001 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3061 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg
     3121 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3181 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3241 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     3301 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     3361 acatgtt
//
