LOCUS       sequence-20668-e         589 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG306GAL-ccdB-HA
FEATURES             Location/Qualifiers
     source          1..589
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(119..243)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             284..373
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     promoter        complement(418..436)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(457..473)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(457..473)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(470..492)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    481..497
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(505..511,512..529,530..535))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    550..571
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cggggagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg
       61 atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca
      121 tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta
      181 atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt
      241 ggtgatgggc tgcaggaatt cgatatcaag cttaggtgga atgtacccat acgatgttcc
      301 tgactatgcg ggctatccct atgacgtccc ggactatgca ggatcctatc catatgacgt
      361 tccagattac gctgctcagt gctaactcga gggggggccc ggtaccagct tttgttccct
      421 ttagtgaggg ttaatttcga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
      481 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
      541 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactg
//
