LOCUS       sequence-20670-e         641 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG306GPD-ccdB-HA
FEATURES             Location/Qualifiers
     source          1..641
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(30..154)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(162..178)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(162..178)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(184..447)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(267..290)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 taaagcctgg gggtgcctaa tgcggccgcc atagtgactg gatatgttgt gttttacagt
       61 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
      121 acgtttctcg ttcagctttt ttgtacaaac ttgtgatggg ggatccacta gttctagaat
      181 ccgtcgaaac taagttctgg tgttttaaaa ctaaaaaaaa gactaactat aaaagtagaa
      241 tttaagaagt ttaagaaata gatttacaga attacaatca atacctaccg tctttatata
      301 cttattagtc aagtagggga ataatttcag ggaactggtt tcaacctttt ttttcagctt
      361 tttccaaatc agagagagca gaaggtaata gaaggtgtaa gaaaatgaga tagatacatg
      421 cgtgggtcaa ttgccttgtg tcatcattta ctccaggcag gttgcatcac tccattgagg
      481 ttgtgcccgt tttttgcctg tttgtgcccc tgttctctgt agttgcgcta agagaatgga
      541 cctatgaact gatggttggt gaagaaaaca atattttggt gctgggattc tttttttttc
      601 tggatgccag cttaaaaagc gggctccatt atatttagtg g
//
