LOCUS       sequence-20672-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG413GAL-ccdB-HA
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(113..237)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             278..367
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      386..633
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(402..420)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(651..670)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(652..670)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(680..697)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(680..696)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(689..711)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 agagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc
       61 tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga
      121 ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa
      181 tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgat
      241 gggctgcagg aattcgatat caagcttagg tggaatgtac ccatacgatg ttcctgacta
      301 tgcgggctat ccctatgacg tcccggacta tgcaggatcc tatccatatg acgttccaga
      361 ttacgctgct cagtgctaac tcgagtcatg taattagtta tgtcacgctt acattcacgc
      421 cctcccccca catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc
      481 cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt
      541 cttttttttc tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag
      601 aaggttttgg gacgctcgaa ggctttaatt tgcggccggt acccaattcg ccctatagtg
      661 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
      721 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
      781 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggacgc
      841 gccctgtagc ggcgca
//
