LOCUS       sequence-20673-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG413GPD-ccdB-HA
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(116..240)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             281..370
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      389..636
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(405..423)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(654..673)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(655..673)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(683..700)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(683..699)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(692..714)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gggagagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg
       61 ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg tcgaccatag
      121 tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt
      181 taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt
      241 gatgggctgc aggaattcga tatcaagctt aggtggaatg tacccatacg atgttcctga
      301 ctatgcgggc tatccctatg acgtcccgga ctatgcagga tcctatccat atgacgttcc
      361 agattacgct gctcagtgct aactcgagtc atgtaattag ttatgtcacg cttacattca
      421 cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag
      481 gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt
      541 tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt
      601 gagaaggttt tgggacgctc gaaggcttta atttgcggcc ggtacccaat tcgccctata
      661 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
      721 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
      781 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga
      841 cgcgccctgt agcggcgcat taag
//
