LOCUS       sequence-20688-e         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG416GAL-ccdB-HA
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(128..252)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             293..382
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      401..648
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(417..435)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(666..685)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(667..685)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctcgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
       61 attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca
      121 ggtcgaccat agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg
      181 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt
      241 gtacaaagtg gtgatgggct gcaggaattc gatatcaagc ttaggtggaa tgtacccata
      301 cgatgttcct gactatgcgg gctatcccta tgacgtcccg gactatgcag gatcctatcc
      361 atatgacgtt ccagattacg ctgctcagtg ctaactcgag tcatgtaatt agttatgtca
      421 cgcttacatt cacgccctcc ccccacatcc gctctaaccg aaaaggaagg agttagacaa
      481 cctgaagtct aggtccctat ttattttttt atagttatgt tagtattaag aacgttattt
      541 atatttcaaa tttttctttt ttttctgtac agacgcgtgt acgcatgtaa cattatactg
      601 aaaaccttgc ttganaaggt tttgggacgc tcgaaggctt taatttgcgg ccggtaccca
      661 attcgcccta tagtgagtcg tattacgcgc gct
//
