LOCUS       sequence-20690-e         645 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG416GPD-ccdB-HA
FEATURES             Location/Qualifiers
     source          1..645
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(29..153)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(161..177)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(161..177)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(183..446)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(266..289)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 aaagcctggg ggtgcctaat gcggccgcca tagtgactgg atatgttgtg ttttacagta
       61 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta
      121 cgtttctcgt tcagcttttt tgtacaaact tgtgatgggg gatccactag ttctagaatc
      181 cgtcgaaact aagttctggt gttttaaaac taaaaaaaag actaactata aaagtagaat
      241 ttaagaagtt taagaaatag atttacagaa ttacaatcaa tacctaccgt ctttatatac
      301 ttattagtca agtaggggaa taatttcagg gaactggttt caaccttttt tttcagcttt
      361 ttccaaatca gagagagcag aaggtaatag aaggtgtaag aaaatgagat agatacatgc
      421 gtgggtcaat tgccttgtgt catcatttac tccaggcagg ttgcatcact ccattgaggt
      481 tgtgcccgtt ttttgcctgt ttgtgcccct gttctctgta gttgcgctaa gagaatggac
      541 ctatgaactg atggttggtg aagaaaacaa tattttggtg ctgggattct ttttttttct
      601 ggatgccagc ttaaaaagcg ggctccatta tatttagtgg atgcc
//
