LOCUS       sequence-20694-e         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG423GAL-ccdB-HA
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(119..243)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             284..373
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      392..639
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(408..426)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(657..676)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(658..676)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(686..703)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(686..702)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cggggaaaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg
       61 atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca
      121 tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta
      181 atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt
      241 ggtgatgggc tgcaggaatt cgatatcaag cttaggtgga atgtacccat acgatgttcc
      301 tgactatgcg ggctatccct atgacgtccc ggactatgca ggatcctatc catatgacgt
      361 tccagattac gctgctcagt gctaactcga gtcatgtaat tagttatgtc acgcttacat
      421 tcacgccctc cccccacatc cgctctaacc gaaaaggaag gagttagaca acctgaagtc
      481 taggtcccta tttatttttt tatagttatg ttagtattaa gaacgttatt tatatttcaa
      541 atttttcttt tttttctgta cagacgcgtg tacgcatgta acattatact gaaaaccttg
      601 cttgagaagg ttttgggacg ctcgaaggct ttaatttgcg gccggtaccc aattcgccct
      661 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gact
//
