LOCUS       sequence-207-exp        8375 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8375)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 8375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L3799
FEATURES             Location/Qualifiers
     source          1..8375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1005..1033)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1781..1801
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        6120..6224
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6225..6293,6294..7085)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6443..6462)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7256..7844
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7745..7764
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7998..8015
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8132..8153
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8168..8173,8174..8191,8192..8198)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8206..8222
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8211..8233
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8230..8246
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8230..8246
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ccgcggtttt ggcgcgccaa atgtaaacct
       61 gtcatttctg tgtatttcag caccgcgaag agcaccataa acagctacaa caagagcacg
      121 aacgtcggct ccaacagaaa aagctgcaaa acgctggtac tcaccacatg ctctgataat
      181 tgccatttcc ttcgatttct gactttcaat tgtgatatga taacctcaaa atctccattc
      241 aaaatgaaaa ctcttaaatt taagagcttt caacaccccg cactttccca cctcatcgtt
      301 tttcccttct atcttatcaa ttttttgtat tttgtgtcag tttgctatat cctcatcacc
      361 ttttttaccg tttaatctct ctatagtatt gttcgggtgt ttcaagatga aacgttttgt
      421 gtgtagcatt tttgaaacac agcgcgaaaa atgcagaaac tatcatccac aatgcattaa
      481 gtgcactcat tgtgatgact cctaaccccc tcgccccacc agttgtctct tcacaaattc
      541 atggcacaat tcaaaaatgg ctttttgtgt tggatcttga tctcgtctct ttccttcttc
      601 tttcgtttct ctcggaccac tcattattcg aatgccatgc atttgttgat gatgcgcagc
      661 gatggtcgca cacactacaa cagatatgat ggtggctgtg cgtggtgcag agctcatgaa
      721 taagtgatgg ggcaccgaga tgtgactcct cccattattc gtcttgagtc gcatcttttg
      781 tcttggtccg ttgtcagtcg agcctcaagg cagccgttcc gccagtgatc gcctgctaag
      841 aagttgtagg tgtagaggaa gaaacgtcgg gtccaaattt caagcaacat ccgcgagatc
      901 gtggatccta tcgattcgcg gccgctgtac acccgggtgc cctatagtga gtcgtattgg
      961 taccggtaga aaaaatgagt aaaggagaag aacttttcac tggagttgtc ccaattcttg
     1021 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
     1081 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
     1141 catgggtaag tttaaacata tatatactaa ctaaccctga ttatttaaat tttcagccaa
     1201 cacttgtcac tactttctgt tatggtgttc aatgcttctc gagataccca gatcatatga
     1261 aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat
     1321 ttttcaaaga tgacgggaac tacaagacac gtaagtttaa acagttcggt actaactaac
     1381 catacatatt taaattttca ggtgctgaag tcaagtttga aggtgatacc cttgttaata
     1441 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg
     1501 aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca
     1561 aagttgtaag tttaaacatg attttactaa ctaactaatc tgatttaaat tttcagaact
     1621 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa
     1681 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat
     1741 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa
     1801 cagctgctgg gattacacat ggcatggacg aactatacaa aagcccacaa gctttggaat
     1861 tcgctagccg gccatacaag taatccgggt tctagaagcg ctgctaaggg ggccctcgtc
     1921 gagtcggtca caatcacctg aaactccaaa ggcagccagt gaggaacgtg aagaagaaga
     1981 aaaagagtca tctgaacagg tttgattttc tttctggtca aaaagatgaa attattgatt
     2041 ttcagccaga tactcccaaa actagcagcg agaagtctgc aagtcgttca cagtcgccca
     2101 gagaatcgcg ggaagtgagc caagaggtat gtttttcaaa aatcaataac tgatcataat
     2161 ttttattgtt tggtgaattt aagaaaataa tattcgaaaa ttcctctgaa ttatcaagat
     2221 tgcagtatta atttcgagaa aaattgagat attcatagag ctattgtaaa ttttcttgat
     2281 ttcagactga aacttcggaa aatcaagaga aaatcaaaga aaaggatgac ggggatgatc
     2341 agcctggcac accgaacagc tatagaagcc gggaaacttc accagctcca aaaaggtcca
     2401 aggagaccag gtttgtcaaa agcttcctgc gattaattct catttcaatt tttcagagaa
     2461 tcagagtctc ctgaaaaatc cccggttcgt tcaagatctc ccagaaggtc ttcagcacgt
     2521 tccccgtcac gatctcctag acggcgccga gaaagaagct cagaaagaaa gcaatccgaa
     2581 gagccagcac cgctaccaga gaaaaagaag aaagagccgc tggatattct acgaacaaga
     2641 accggaggag catatattcc acccgccaaa cttcgactta tgcaacaaca gattagtgat
     2701 aagcaaagtg aacagtatca gagaatgaat tgggaaagaa tgaagaaaaa gattcacgga
     2761 ttggttaaca gagtcaacgc gaagaatctt gttcaaattg tcagagaact tcttcaagag
     2821 aatgtgattc gttcaaagtg agtgagaaaa tcgaaggaaa aggaaagaat taatttaatt
     2881 tttcagggga cttctctgcc gtgacattat tcaagctcag gctttctcac caggattctc
     2941 taacgtctat gcagctttgg cggcagttat caactcgaaa ttccctcatg tcggtgaact
     3001 tcttctccgt cgtctgattg tacagttcaa aagaagtttc cgtagaaatg acagaggcgt
     3061 cacggtgaac gtgatcaaat tcatcgcaca tttgattaat caacaagttg ctcacgaagt
     3121 tcttgcgctg gaaatcatga ttctgatgct tgaagaacca actgatgatt cagttgaagt
     3181 cgccattgcg ttcctgaaag agtgtggagc aaagcttctg gagattgctc cagcagctct
     3241 taacagtgtc tacgaccgtc ttcgtgcaat tctcatggaa actgaaagat cggaaaatgc
     3301 actggatcga cgtattcagt atatgattga gactgcaatg cagattcgaa aggacaaatt
     3361 tgcggcgtat ccggcggtca ttgaagactt ggacttgatt gaggaggagg atcagatcat
     3421 ccatacactt aatttggagg atgcggttga tccggaaaat gggcttaatg tgttcaaact
     3481 agatccagaa ttcgaaaaga acgaggaggt ttatgaggag atccgtaagg aaatcattgg
     3541 aaacgccgat atttcggatg aggatggtgg cgacgagttg gatgatgaag aagagggtag
     3601 tgatgtggaa gaggctccga agaagactac agagattatt gataatactg atcagaattg
     3661 actgctttca gaaggtattc attttgagtt ttgggccggc aaatctgtaa gttgccggtt
     3721 gccgaaaatt tgctgaattt gccggaaaaa aaaattccgg aatttattta aaaacttttt
     3781 gtaaaaatta aattaaattt gcaacttttc agagaagtct acctgacaat gcaatcatct
     3841 ttggactacc aagaagctgc tcacaaattg ctgaaaatga agattccaga cagcatgcag
     3901 gtcagcgatg ttgcaaagaa aaattttcga ccaaaaaaac caaccaatca taaaatttaa
     3961 aaaaaaactc cgtttttttc ttttttttta tacgagaaaa accaaaaaaa tgtatttttg
     4021 ccaaattcta aaatactatc cccgaaattt tcaatatttt ctctttcaga acgaactctg
     4081 cgcgatgctt gtcgattgtt gtgctcaaca gcgtacctac gagcgattct acggaatgct
     4141 catcgaacgt ttctgccgac ttcgcctcga ataccagcaa tactttgaaa agctctgcca
     4201 ggacacgtat tccacgattc accgaattga catcacaaaa ctgcggaatt tggctcgcct
     4261 tattgctcat ttgctctcga cggatgctat tgactggaag attttggccg atatgaaaat
     4321 gaccgaagag gacacaactt cttctggcag aatctatatt aaatatatat ttaatgaact
     4381 tgtggaggcg atgggaatgg ttaaacttca ttcgagagtt actgatccgt gagtttccta
     4441 gagagagttg ttttcgtatt caattttccc tattttcaga actttggctc attgctttgt
     4501 tggattattc ccacgaacta atccgaacag cgcacgattt tcgatcaact tcttcacaat
     4561 gattggattg ggtggtttga cgttggaact tcgtgaatgg ctggcaaagg gtctcaagaa
     4621 gaagaaggga atgctggatc agttgaaggc cgaatcaagc tcagattcat cgtcgtcttc
     4681 ggattcgtca gactcgtctg attcttcgga ttctgacgat tcatccgact cgtcttcaga
     4741 ttcctcatct tcttcagaat cagagccaga accaccgaag aaaaagaaga agaagaacag
     4801 tgaagagagt tccaaaaaga aggaaaaaga gaatattggt cgacgggatc gtggagacaa
     4861 gagagctgaa cgtcatcgtg atcaaagtgt ggagaacaag gacaaggatc gtcgacgtcg
     4921 ccaggattct gacgaaaatc gtcggccaga acgaggagat gaccgcaagg atcggagtaa
     4981 agatcgtcgt cgtcaagact cggatgatga ggatcggaaa ggtcgtgaac gtcgggaaga
     5041 ttcaggggaa agacgtcgcg gagatcggga tcgacgtgat cgaaacaagg atcaggagga
     5101 tcaccgtgaa gatcgccgtg accgaagcaa ggatcgtgag gatcgacgtg atcgccgtcg
     5161 tcatgactct gatgatgatc gtaaaactcg tcgggataga agtgaagagc gaggaggacg
     5221 tcgtcgtgaa gtggaatcgg atgatcgacg ccgacgtcgt tgaattttca aattttaaat
     5281 actgaatatt tgtttttttt cctattattt atttattctc tttgtgtttt ttttcttgct
     5341 ttctaaaaaa ttaattcaat ccaaatctaa acattttttt ttctctttcc gtctcccaat
     5401 tcgtattccg ctcctctcat ctgaacacaa tgtgcaagtt tatttatctt ctcgctttca
     5461 tttcattagg acgtgggggg aattggtgga agggggaaac acacaaaagg atgatggaaa
     5521 tgaaataagg acacacaata tgcaacaaca ttcaattcag aaatatggag gaaggtttaa
     5581 aagaaaacat aaaaatatat agaggaggaa ggaaaactag taaaaaataa gcaaagaaat
     5641 taggcgaacg atgagaattg tcctcgcttg gcaaatgcga atccgtatgg agaggcacgt
     5701 ttggcgaagg caaatgttcg gtatggagat ctgtaaaaat ttttaagttg aaatttggtg
     5761 ttgctctttt acaaaatttt ccgattttcg cttgaaatta cggtgccagg tctcgacacg
     5821 tcttccaatt tttcaaattc aaaagagcct ttaatgggct gtagttgcta atttctcgtt
     5881 tttgaaaatt tttcttccgt ttaatcgaaa tttgatgtat tttatttatg attttcaata
     5941 aatttcaaag aaactggtga aaactcggaa aattgtgaac tacagtaatc caatccttaa
     6001 aggcgcacac cttttaaatg tccgccccaa tacgatattt ttttaagatt cgctagagcg
     6061 gcccaaaagg cctttttttt ttggcgccga cgtcaggtgg cacttttcgg ggaaatgtgc
     6121 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     6181 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     6241 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     6301 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     6361 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     6421 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     6481 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     6541 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     6601 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     6661 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     6721 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     6781 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     6841 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     6901 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     6961 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     7021 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     7081 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     7141 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     7201 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7261 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     7321 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     7381 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     7441 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     7501 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     7561 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     7621 ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa
     7681 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     7741 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     7801 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     7861 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     7921 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     7981 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     8041 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     8101 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     8161 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     8221 aatttcacac aggaaacagc tatgaccatg attacgccaa gctgtaagtt taaacatgat
     8281 cttactaact aactattctc atttaaattt tcagagctta aaaatggctg aaatcactca
     8341 caacgatgga tacgctaaca acttggaaat gaaat
//
