LOCUS       sequence-207008-        4053 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-65
FEATURES             Location/Qualifiers
     source          1..4053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     30..47
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1967..1985)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2053..2157
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2379..3170
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3341..3929
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3830..3849
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
       61 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatca ctctgtggtc
      121 tcaaatgatg gctgaagccg gtgacgagaa tctttacgcc gccgccagag acatcgccag
      181 agctctcggc aaggatccaa gtgccgccgg agatatctta cagattctct ccggttatgg
      241 cgcttccgga aaccgcggcg gagatcctcg acccacccca tcccgcggcg gctcaaacgt
      301 caactttgac cgagccctca cctctttaga gagacagatc tccagctaca tcgtggaaga
      361 tcgaccgatc tggtctgatc ccgttgactc gcgcaccttc ctcgactctg ttgatgagct
      421 acttgcgatt gccggagact tgagatccat ggccggagat aaatccgtcg ccgtctgtca
      481 atcccgtgcc gatgagctga ttcaacaggt gatgtttagg cttcaagaag agtttggatt
      541 tgtaatggac cgagcccctg actcgtttga ctcggacgat gagttccccg gcgaagagga
      601 caacgacaca agcgacgggg taatcgttgc tcgtccaatc actgattaca aaatcgtgat
      661 cgaggcgtta caatctagcg ttattggcga tctaaacgcg atcgctgtgc gcatggtcgc
      721 tggtggattt gcaaaagagt gttcaagagt gtacagcagt cgacgaagag agtttctaga
      781 ggagagtttg tcgaggttac atctcagagg actaagtatg gaggaagtgc aagagagtcc
      841 atggcaagat cttgaggacg aaatcgaccg ttggattaaa gccgtaacgc tgatattcca
      901 cgttttcttc ccgagcgagc ggcttctctg cgatcgcgtt ttctctgatc ttcctgtttc
      961 ctctgtaacc gatctttctt tcatggaagt ttgtcgcgga accacaacac agcttctgaa
     1021 tttcgcagac gcgatcgctt taggaagccg tttacctgaa cgtttgttta aggttgtgga
     1081 tttatacgaa gcgatgcaag accttattcc taagatggaa acgctatttt ctgatcgata
     1141 ttgttcgccg ctaaggcatg aggcgctcgc gattcataaa cggttaggag aagcgattag
     1201 agggatattt atggagctgg agaatttaat ccgacgtgac cctcccaaaa cagcgtttcc
     1261 tggcggtggg attcacccga tcacgcggta cgtgatgaat tatctgcgtg cggcgtgtaa
     1321 atcgcggcaa tcgttggagc agatacttga ccagacagga aatgaaaccg gatcagatac
     1381 cagaccgttg tctgtgcaaa tcatatgggt tttggagttg ttggagagta acttggaagg
     1441 caagaaaaga acttatcgtg atccgtcttt gtgtttcttg ttcatgatga acaatgacaa
     1501 gtatattctt gataaggcta aggataacga gctaggttta gtcctaggag aagattggat
     1561 tgtgaagcac gcagcgaaac tgaggcaata ccattctaat taccgaagga gctcatggaa
     1621 ccaagtggtg ggattgctta ggaccgatgg tccgtatccc aagttagtag agaacctaag
     1681 attgttcaaa tcacagttcg atgaggtgtg taaggtgcaa tctcaatggg ttgttagtga
     1741 tgggcaattg agggaggagt taagaagctc tgttgccgga attgtatctc cggcgtactc
     1801 gaatttcatc agaagattga aggagtcacc ggagattaat gggaggcgtg gagagccgtt
     1861 tattccgtat actgttgaag atgtggagtt tataatcaaa cggttgttca aggaaagctc
     1921 aagttgagct ttgagaccac gaagtggctc ttcagtggac gaaagggcct cgtgatacgc
     1981 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     2041 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2101 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     2161 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     2221 gttttcatgg cttgttatga ctgttttttt ttggggtaca gtctatgcct cgggcatcca
     2281 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
     2341 cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gagggaagcg gtgatcgccg
     2401 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
     2461 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagcgata
     2521 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
     2581 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
     2641 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
     2701 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
     2761 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
     2821 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
     2881 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
     2941 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
     3001 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     3061 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
     3121 aatttgtcca ttacgtaaaa ggcgagatca ccaaggtagt cggcaaataa ctgtcagacc
     3181 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     3241 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3301 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3361 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3421 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3481 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3541 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3601 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3661 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3721 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3781 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3841 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3901 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3961 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     4021 ataaccgtat taccgccttt gagtgagctg ata
//
