LOCUS       sequence-207009-        3261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-349
FEATURES             Location/Qualifiers
     source          1..3261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     28..45
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1173..1191)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1259..1363
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1585..2376
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2547..3135
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3036..3055
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
       61 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact ctgtggtctc
      121 aaatggaaaa ttcatcagag tcctcattac cggatttgga ttcagctctg aaactgaaga
      181 gatttgaacc aaaagagttg gagcaagcaa cagattcatt caacagtgcc aacatcattg
      241 gctcaagcag cttaagcaca gtgtacaaag gtcagctaga agatgggaca gtgattgcag
      301 taaaagtatt gaatctaaag gaattctctg cagaatcaga caagtggttc tacacagaag
      361 ctaaaacatt gagccaacta aaacatcgaa acctggtgaa gatcttaggg tttgcgtggg
      421 aaagcggcaa aacgaaagct ttagtgcttc catttatgga gaatggaaac ttggaggaca
      481 ccattcacgg ctctgcagca ccgattgggt cgcttttaga aaaaatcgat ctttgtgttc
      541 atatcgcaag cggaatcgat tatcttcatt ctggatatgg ttttcccatc gttcattgtg
      601 atctgaagcc agctaatata ctccttgaca gtgaccgcgt tgctcacgta agcgattttg
      661 gaactgctcg gattctaggt ttccgcgaag atggaagcac cacagcttca acatcagcct
      721 tcgagggtac aattggatac ttagctccag agtttgctta tatgaggaaa gtgacaacaa
      781 aagccgatgt attcagcttc gggatcataa tgatggagct gatgacgaaa cagaggccaa
      841 cttcgttgaa tgatgaagat tcacaagaca tgactttgcg ccaattggtg gagaaatcga
      901 ttggaaatgg aagaaaaggg atggttaggg ttcttgatat ggaactcggg gactctattg
      961 tttctctgaa acaggaagag gctattgaag attttctgaa gctttgtttg ttctgtacaa
     1021 gctctagacc tgaagatcga cctgatatga acgagattct tacacatctg atgaaactta
     1081 gaggcaaagc gaattcattt cgagaagatc gtaacgagga tcgagaagtt taggctttga
     1141 gaccacgaag tggctcttca gtggacgaaa gggcctcgtg atacgcctat ttttataggt
     1201 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     1261 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     1321 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgcgct cacgcaactg
     1381 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     1441 ttatgactgt ttttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt
     1501 tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt
     1561 cgccctaaaa caaagttaaa catcatgagg gaagcggtga tcgccgaagt atcgactcaa
     1621 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat
     1681 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gcgatattga tttgctggtt
     1741 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa
     1801 acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg
     1861 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg
     1921 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct
     1981 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa
     2041 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta
     2101 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc
     2161 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca
     2221 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt
     2281 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccattac
     2341 gtaaaaggcg agatcaccaa ggtagtcggc aaataactgt cagaccaagt ttactcatat
     2401 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     2461 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     2521 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2581 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2641 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     2701 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2761 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2821 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2881 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     2941 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3001 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3061 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     3121 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3181 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     3241 gcctttgagt gagctgatac c
//
