LOCUS       sequence-207026-        3216 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-351
FEATURES             Location/Qualifiers
     source          1..3216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..85
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1168..1186)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1254..1358
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1580..2371
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2542..3130
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3031..3050
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
       61 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
      121 ccccgcgcgt tggccgattc attaatcact ctgtggtctc aaatgggaag caagtattcc
      181 aaggcaacaa attccataaa tgatgcttta agctcgagtt atctcgttcc ttttgaaagt
      241 tatcgagttc ctttagtaga tttggaggaa gcaactaata attttgatca caagttttta
      301 attggacatg gtgtctttgg gaaggtttac aagggtgttt tgcgtgatgg agcaaaggtg
      361 gccctgaaaa ggcgtacacc tgagtcctca caaggtattg aagagttcga aacagaaatt
      421 gagactctct cattttgcag acatccgcat ctggtttcat tgataggatt ctgtgatgaa
      481 agaaatgaga tgattctaat ttataaatac atggagaatg ggaacctcaa gagacatttg
      541 tatggatcag atctacccac aatgagcatg agctgggagc agaggctgga gatatgcata
      601 ggggcagcca gaggtctaca ctaccttcat actagagcaa ttatacatcg tgatgtcaag
      661 tctataaaca tattgcttga tgagaatttt gtgccaaaaa ttactgattt tggaatatcc
      721 aagaaaggga ctgagcttga tcaaacccat cttagcacag tagtgaaagg aactctcggc
      781 tacattgacc ctgaatattt tataaaggga cgactcactg aaaaatctga tgtttattct
      841 ttcggtgttg ttttattcga agttctttgt gctaggtctg ccatagttca atctcttcca
      901 agggagatgg ttaatttagc tgaatgggca gtggagtcgc ataataatgg acagttggaa
      961 caaatcgtag atcccaatct tgcagataaa ataagaccag agtccctcag gaagtttgga
     1021 gatacagcgg taaaatgctt agctttgtct agtgaagata ggccatcaat gggtgatgtg
     1081 ttgtggaaac tggagtatgc actccgtctc caagagtctg ttatttaagc tttgagacca
     1141 cgaagtggct cttcagtgga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     1201 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     1261 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     1321 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gcgctcacgc aactggtcca
     1381 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     1441 actgtttttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     1501 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     1561 taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc
     1621 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     1681 cggctccgca gtggatggcg gcctgaagcc acacagcgat attgatttgc tggttacggt
     1741 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     1801 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     1861 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     1921 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     1981 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     2041 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     2101 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     2161 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     2221 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     2281 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc attacgtaaa
     2341 aggcgagatc accaaggtag tcggcaaata actgtcagac caagtttact catatatact
     2401 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     2461 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     2521 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     2581 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     2641 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     2701 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     2761 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     2821 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     2881 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     2941 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3001 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3061 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3121 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     3181 tgctcacatg ttctttcctg cgttatcccc tgattc
//
