LOCUS       sequence-207041-        2304 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-41308
FEATURES             Location/Qualifiers
     source          1..2304
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     88..110
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     102..119
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     103..119
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    120..176
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(189..205)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(189..205)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(202..224)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    213..229
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(237..243,244..261,262..267))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    282..303
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(447..464)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(618..1206)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(698..717)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1377..2168)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tgtaggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
       61 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
      121 aattcgagct cggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttggcg
      181 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
      241 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      301 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcga
      361 agacaacatt tgagaccaca gagtgattaa tgaatcggcc aacgcgcggg gagaggcggt
      421 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      481 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      541 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      601 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      661 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      721 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      781 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
      841 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
      901 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
      961 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1021 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     1081 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1141 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     1201 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     1261 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     1321 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttat
     1381 ttgccgacta ccttggtgat ctcgcctttt acgtaatgga caaattcttc caactgatct
     1441 gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag cctgtctagc ttcaagtatg
     1501 acgggctgat actgggccgg caggcgctcc attgcccagt cggcagcgac atccttcggc
     1561 gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc
     1621 tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca
     1681 aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca
     1741 acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc
     1801 tcgaagatac ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta
     1861 gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg
     1921 agaatctcgc tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc
     1981 cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc gctgtgtggc
     2041 ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga
     2101 tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct
     2161 tccctcatga tgtttaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg
     2221 ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc
     2281 atagactgta ccccaaaaaa aaac
//
