LOCUS       sequence-207051-        2286 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pJOG824
FEATURES             Location/Qualifiers
     source          1..2286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     4..23
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     257..274
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             364..384
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(427..445)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        513..617
                     /gene="bla"
                     /label=AmpR promoter
     CDS             839..1630
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(1801..2286,1..103)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
       61 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
      121 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
      181 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
      241 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
      301 accgcctctc cccgcgcgtt ggccgattca ttaatcactc tgtggtctca ttcggctgca
      361 gcccctaaga agaagagaaa ggtttgagct ttgagaccac gaagtggctc ttcagtggac
      421 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
      481 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
      541 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
      601 attgaaaaag gaagagtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
      661 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ttggggtaca
      721 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
      781 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat
      841 gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
      901 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
      961 cctgaagcca cacagcgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     1021 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     1081 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     1141 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     1201 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     1261 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     1321 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     1381 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     1441 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     1501 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     1561 gcgcgcagat cagttggaag aatttgtcca ttacgtaaaa ggcgagatca ccaaggtagt
     1621 cggcaaataa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     1681 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     1741 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     1801 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     1861 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     1921 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     1981 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     2041 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     2101 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     2161 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     2221 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     2281 gcttcc
//
