LOCUS       sequence-207056-        2793 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-UBC20
FEATURES             Location/Qualifiers
     source          1..2793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     36..53
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(713..731)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        799..903
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1125..1916
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2087..2675
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2576..2595
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
       61 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatcactct
      121 gtggtctcaa atggccgccg taaatggata tcagggaaat actccggcag atcctccggc
      181 gtctaatgga tcaaaacaac ctgcggctcc gaccaagact gttgacagcc aatctgttct
      241 caaaagactg caatctgaat tgatgggctt gatgatgggc ggtggtccag gaatatcggc
      301 tttccctgag gaggacaaca tattctgctg gaaagggaca ataacaggaa gcaaagatac
      361 tgtgtttgaa ggaactgagt acagactctc actctctttc tccaatgact atcctttcaa
      421 gcctccaaag gttaagtttg agacatgttg cttccacccc aatgttgatg tctatggcaa
      481 tatctgctta gacattcttc aggataagtg gtcatctgct tacgatgtga ggacgatact
      541 actatcgatt cagagcctcc tgggagaacc gaacatcagt tcaccattga acactcaagc
      601 agctcagctt tggagtaacc aagaagagta caggaagatg gttgagaagc tttacaagcc
      661 tccaagtgca tgagctttga gaccacgaag tggctcttca gtggacgaaa gggcctcgtg
      721 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
      781 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      841 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      901 agtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
      961 gtggcggttt tcatggcttg ttatgactgt ttttttttgg ggtacagtct atgcctcggg
     1021 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     1081 atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgagg gaagcggtga
     1141 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     1201 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     1261 gcgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     1321 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     1381 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     1441 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     1501 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     1561 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     1621 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     1681 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     1741 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     1801 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     1861 tggaagaatt tgtccattac gtaaaaggcg agatcaccaa ggtagtcggc aaataactgt
     1921 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     1981 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     2041 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2101 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2161 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2221 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2281 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2341 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2401 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2461 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2521 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2581 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2641 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2701 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2761 ctgtggataa ccgtattacc gcctttgagt gag
//
