LOCUS       sequence-207065-        4023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pJOG658
FEATURES             Location/Qualifiers
     source          1..4023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     32..51
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     285..302
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             384..473
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             495..2141
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
                     IEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
                     NDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
                     YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
                     DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
                     QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
                     LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
                     YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
                     QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
                     VDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(558..576)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     primer_bind     complement(2192..2210)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2278..2382
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2604..3395
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3566..4023,1..131)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
       61 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      121 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      181 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
      241 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
      301 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
      361 aatcactctg tggtctcacc atgtacccat acgatgttcc tgactatgcg ggctatccct
      421 atgacgtccc ggactatgca ggattgtatc catatgacgt tccagattac gccactagag
      481 ctgctgtgtc tgctgaggac gccaaaaaca taaagaaagg cccggcgcca ttctatcctc
      541 tagaggatgg aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc
      601 ctggaacaat tgcttttaca gatgcacata tcgaggtgaa catcacgtac gcggaatact
      661 tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca
      721 gaatcgtcgt atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat
      781 ttatcggagt tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca
      841 gtatgaacat ttcgcagcct accgtagtgt ttgtttccaa aaaggggttg caaaaaattt
      901 tgaacgtgca aaaaaaatta ccaataatcc agaaaattat tatcatggat tctaaaacgg
      961 attaccaggg atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta
     1021 atgaatacga ttttgtacca gagtcctttg atcgtgacaa aacaattgca ctgataatga
     1081 attcctctgg atctactggg ttacctaagg gtgtggccct tccgcataga actgcctgcg
     1141 tcagattctc gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga
     1201 ttttaagtgt tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga
     1261 tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ttacgatccc
     1321 ttcaggatta caaaattcaa agtgcgttgc tagtaccaac cctattttca ttcttcgcca
     1381 aaagcactct gattgacaaa tacgatttat ctaatttaca cgaaattgct tctgggggcg
     1441 cacctctttc gaaagaagtc ggggaagcgg ttgcaaaacg cttccatctt ccagggatac
     1501 gacaaggata tgggctcact gagactacat cagctattct gattacaccc gagggggatg
     1561 ataaaccggg cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg
     1621 ataccgggaa aacgctgggc gttaatcaga gaggcgaatt atgtgtcaga ggacctatga
     1681 ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat
     1741 ggctacattc tggagacata gcttactggg acgaggacga acacttcttc atagttgacc
     1801 gcttgaagtc tttaattaaa tacaaaggat atcaggtggc ccccgctgaa ttggaatcga
     1861 tattgttaca acaccccaac atcttcgacg cgggcgtggc aggtctgccc gacgatgacg
     1921 ccggtgaact tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag
     1981 agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg
     2041 tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag
     2101 agatcctcat aaaggccaag aagggcggaa agtccaaatt ggctagtgcc gcaatgtgag
     2161 accacgaagt ggctcttcag tggacgaaag ggcctcgtga tacgcctatt tttataggtt
     2221 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     2281 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     2341 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgcgctc acgcaactgg
     2401 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
     2461 tatgactgtt tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     2521 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     2581 gccctaaaac aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     2641 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     2701 tgtacggctc cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta
     2761 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     2821 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     2881 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     2941 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     3001 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     3061 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     3121 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     3181 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     3241 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     3301 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg
     3361 taaaaggcga gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata
     3421 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     3481 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     3541 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3601 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3661 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3721 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3781 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3841 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3901 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3961 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     4021 tcg
//
