LOCUS       sequence-207068-        9690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9690)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 9690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-299
FEATURES             Location/Qualifiers
     source          1..9690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     112..131
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     215..234
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        215..233
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    234..258
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             312..329
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3877..3966
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             3979..4068
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(4839..4857)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4853..4900
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4937..5392
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5024..5043)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5234..5255
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5484..6299)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5484..6299)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6207..6226
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6291..6310
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6421..7009
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6910..6929
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7163..7180
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7195..7334
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7260..7279)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7420..7442
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7436..7627)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8436..9518)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9480..9499
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9519..9596)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac
       61 gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc
      121 ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc
      181 ggcgtagagg atcgagatct cgatcccgcg aaattaatac gactcactat aggggaattg
      241 tgagcggata acaattcccc tctagaaata attttgcctt aactttaaga aggagatata
      301 ccatgggcag ccaccaccac caccaccaca gcggaatgca gatctttgtt aagactctca
      361 ccggaaagac aatcacccta gaggtggaaa gctccgacac catcgacaac gttaaggcca
      421 agatccagga taaggagggc attcctccgg atcagcagag gcttattttc gccggcaagc
      481 agctagagga tggccgtacg ttggctgatt acaatatcca gaaggaatcc accctccact
      541 tggtcctcag gctccgtggt ggttaagctt gcaattaact ttaagaagga gatatacaat
      601 gcttcacaag cgagctagtg aagctaacga taagaacgat aataccatca tcggttccga
      661 tttagcatca tctaagaaac gccgtatcga tttcactgaa tcttcgtctg ataagtcatc
      721 ttcgatttta gcttctggta gtagcagggg ttttcacggc gatagcgtcg ttcagcaaat
      781 cgacatggct tttggtaatt cgaaccgtca ggagattgat gaagatctgc acagtcgcca
      841 gctcgccgtc tatggtcgtg agactatgag gcgtctcttt gcttccaatg ttctcatctc
      901 ggggatgcac ggtcttggtg ccgagattgc caagaatctt atacttgctg gtgtgaagtc
      961 tgtgaccttg catgatgaaa gagttgtaga gctatgggac ttatcaagca actttgtttt
     1021 ctcggaggat gatgttggca agaatagagc agatgcttct gttcagaagt tacaggatct
     1081 taacaatgct gtggttgtct ctagcttgac caaaagctta aacaaagagg atctttctgg
     1141 cttccaggtt gttgtattct ctgacataag catggaaaga gcaatcgaat ttgatgacta
     1201 ttgtcacagc catcagcctc ctatcgcttt tgtcaaggct gatgtcaggg gtcttttcgg
     1261 ctctgtcttt tgtgattttg ggcctgaatt tgcggttctt gatgttgatg gagaggaacc
     1321 ccatacaggc atcattgcgt ctatctctaa tgagaaccag gcatttattt cctgtgtcga
     1381 cgatgagagg cttgagtttg aagatggtga cctagttgtt ttctcagaag ttgagggtat
     1441 gacagaattg aatgatggga aaccaaggaa gataaaaagt acacggccat attcattcac
     1501 ccttgatgag gacactacta actatggaac atatgtgaag ggtggtattg tcactcaagt
     1561 gaaacagcca aagttactga atttcaagcc attgagggaa gcgcttaagg atccagggga
     1621 ttttctattt agtgatttct ctaagtttga tcggcctcca ctccttcact tagcattcca
     1681 ggcacttgat cattttaaag ctgaagctgg cagatttcct gttgctggct cagaagagga
     1741 cgctcagaag cttatatcga ttgccacagc catcaatacc gggcagggtg atttaaaggt
     1801 ggagaatgtt gatcagaagc ttctaagaca cttttccttt ggagccaagg ctgtcctgaa
     1861 tcctatggct gcaatgtttg gcggtattgt tggacaggag gttgttaaag cttgctctgg
     1921 aaaatttcat cctctcttcc agtttttcta ctttgattca gtggagtcac tcccctctga
     1981 acctgtggac tcgagtgact ttgcaccaag aaacagccgg tatgatgccc aaatatctgt
     2041 atttggggcc aagttccaga agaaacttga agatgctaaa gttttcacag tagggtctgg
     2101 tgctcttggc tgtgagttct tgaaaaattt ggctctgatg ggggtttctt gtggaagcca
     2161 agggaagctg acagtgacag atgatgatat cattgagaag agtaacctca gtcgtcaatt
     2221 tctgttccgt gactggaaca ttggacaggc taaatccaca gttgctgctt cggctgctgc
     2281 tgttataaat cctcgcttca acattgaggc cctgcaaaac cgtgttggcg cggagactga
     2341 gaatgtattt gacgatgcct tctgggagaa cttaactgtt gtcgtcaatg cactggataa
     2401 tgttaatgcg aggctctatg ttgattcgag gtgcttgtat ttccagaagc ctctccttga
     2461 gtctggaact cttggtacaa agtgcaacac acagtcggtt atcccacatc taactgaaaa
     2521 ttacggtgcc tcaagggacc caccagagaa acaggccccc atgtgtacag tgcattcctt
     2581 cccgcataac attgatcact gtttaacttg ggctcgctct gagtttgagg gtctgctgga
     2641 gaaaactccc gctgaagtga atgcatatct ttctagccca gttgagtaca ctaactcaat
     2701 gatgagtgct ggtgatgctc aggcgaggga cacattggag aggattgttg agtgccttga
     2761 aaaggagaag tgtgaaacct tccaagactg cttaacctgg gcacgactga ggtttgagga
     2821 ttactttgtg aaccgtgtca agcaattgat atacacattt cctgaagatg ctgcgactag
     2881 taccggagct ccattctggt ctgctccaaa aagattccct cgtccactcc agtactcgtc
     2941 gtccgaccca agtctcctta actttatcac agcgactgct attttacgag cagagacatt
     3001 tgggatcccg atacctgagt ggactaagaa cccaaaggaa gcagcagaag ctgttgacag
     3061 agtgatagtc ccagactttg agccaaggca agatgcaaag attgtgactg atgagaaagc
     3121 taccacttta accactgctt cggtggacga cgctgcagtc atcgacgatc tcattgctaa
     3181 gatcgaccag tgtaggcata acttgtcgcc agacttcagg atgaaaccta ttcagttcga
     3241 aaaggatgat gatacaaact atcacatgga tgtgatagca ggactagcca acatgagggc
     3301 gagaaattac agcatccctg aagtggacaa gctgaaagca aagttcatag cagggagaat
     3361 catcccagcc attgcgactt caacagccat ggctactggg ctggtctgcc tcgagctgta
     3421 caaggtcctt gatggaggac ataaagtgga agcttacaga aacacatttg ccaacctggc
     3481 acttccacta ttctccatgg ctgaaccact tccaccaaag gtggtgaagc accgtgacat
     3541 ggcttggaca gtttgggaca gatgggttct caagggaaac ccaactcttc gtgaggtgtt
     3601 gcagtggctg gaggacaaag ggcttagcgc atacagcatc tcttgtggaa gctgtctact
     3661 gttcaacagt atgttcacaa ggcacaagga gaggatggac aagaaggttg tggatctcgc
     3721 tcgggatgtt gcgaaagtcg agttaccgcc ttatcgcaac catcttgatg tagtggtggc
     3781 ttgtgaggat gaagatgaca atgacgtcga tattcctctc gtctctatct acttcaggtg
     3841 agcttactat taactttaag aaggagatat accatgtacc catacgatgt tcctgactat
     3901 gcgggctatc cctatgacgt cccggactat gcaggattgt atccatatga cgttccagat
     3961 tacgccacta gagctgctta cccatacgat gttcctgact atgcgggcta tccctatgac
     4021 gtcccggact atgcaggatt gtatccatat gacgttccag attacgcaat gtgttctcat
     4081 aatgaaaaca tcgattcccg aaaattcaca aaatcagaac cttgtcatat taaaaaaatc
     4141 tcaatttcaa ttctctcgat ttctctatca tcatcatcca tggcttcgca agctagcctt
     4201 ctccttcaga aacaactcaa agatctctgt aagcatcctg ttgatggatt ctctgctgga
     4261 ctcgttgatg agaagaatat attcgaatgg agcgttacca ttatcggacc tcctgatact
     4321 ctctatgaag gaggattctt taacgcgatt atgacatttc cgcaaaacta tccgaatagt
     4381 ccgccaaccg tgaggtttac ttcggatatg tggcatccta acgtttattc tgatggtcgt
     4441 gtttgcatat ctattcttca tcctcccggt gatgatccta gcggttatga gcttgcaagc
     4501 gagcgttgga ctcctgttca tacagttgag agtattatgt tgagtatcat atcaatgctt
     4561 tctggtccta acgatgagtc tccagcaaat gttgaagctg ccaaagaatg gcgagataag
     4621 agagatgagt tcaagaagaa agtgagccgt tgtgtcagaa agtctcaaga aatgttttga
     4681 gcttttacgc ttttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     4741 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgggatgag atccggctgc
     4801 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
     4861 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
     4921 cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     4981 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     5041 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     5101 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     5161 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     5221 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     5281 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     5341 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     5401 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     5461 tgtatccgct catgaattaa ttcttagaaa aactcatcga gcatcaaatg aaactgcaat
     5521 ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga
     5581 gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg
     5641 actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt
     5701 gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagttt atgcatttct
     5761 ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc
     5821 aaaccgttat tcattcgtga ttgcgcctga gcgagtcgaa atacgcgatc gctgttaaaa
     5881 ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca
     5941 atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc
     6001 gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga
     6061 ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg
     6121 ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag
     6181 attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca
     6241 tccatgttgg aatttaatcg cggcctagag caagacgttt cccgttgaat atggctcata
     6301 acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga ccaaaatccc
     6361 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6421 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6481 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6541 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     6601 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6661 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6721 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6781 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6841 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6901 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6961 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7021 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     7081 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     7141 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
     7201 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt
     7261 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     7321 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     7381 tgctcccggc atccgcttac agacaagctg tgaccgactc cgggagctgc atgtgtcaga
     7441 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     7501 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     7561 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     7621 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     7681 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     7741 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     7801 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     7861 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     7921 acgaaacacg gaaacccaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     7981 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     8041 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggggccg
     8101 ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga
     8161 aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg
     8221 cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta
     8281 cgagttgcat gataaagaac acagtcataa gtgcggcgac gatagtcatg ccccgcgccc
     8341 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct
     8401 aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     8461 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     8521 ttgggcgcca gggtggtttt tcttttcacc agtgacacgg gcaacagctg attgcccttc
     8581 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga
     8641 aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcctc ggtatcgtcg
     8701 tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt
     8761 gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc
     8821 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
     8881 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
     8941 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
     9001 tgctccacgc ccagtcgcgt accgtcctca tgggagaaaa taatactgtt gatgggtgtc
     9061 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
     9121 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
     9181 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
     9241 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
     9301 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
     9361 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
     9421 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
     9481 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc
     9541 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg
     9601 atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
     9661 gccgttgagc accgccgccg caaggaatgg
//
