LOCUS       sequence-207079-       12650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of L2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12650)
  AUTHORS   Yuzawa S, Deng K, Wang G, Baidoo EE, Northen TR, Adams PD, Katz L, 
            Keasling JD
  TITLE     Comprehensive in Vitro Analysis of Acyltransferase Domain Exchanges 
            in Modular Polyketide Synthases and Its Application for Short-Chain 
            Ketone Production.
  JOURNAL   ACS Synth Biol. 2017 Jan 20;6(1):139-147. doi: 
            10.1021/acssynbio.6b00176. Epub 2016 Sep 6.
  PUBMED    27548700
REFERENCE   2  (bases 1 to 12650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.6b00176"; journalName: "ACS Synth Biol"; date: 
            "2017-01-20- 20"; volume: "6"; issue: "1"; pages: "139-147"
COMMENT     Sequence Label: pSY075
FEATURES             Location/Qualifiers
     source          1..12650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(99..118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     309..330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(560..1375)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(560..1375)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1283..1302
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1367..1386
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1497..2085
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1986..2005
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2239..2256
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2271..2413
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2339..2358)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2499..2521
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2515..2706)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3515..4597)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4559..4578
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4598..4675)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     4881..4900
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4988..5007
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4988..5006
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5007..5031
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5046..5068
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5088..5105
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5115..5132
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             12494..12511
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(12564..12582)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      12578..12625
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat
      601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
      661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
      721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
      781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
      841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
      901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
      961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
     1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
     1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac
     1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa
     1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta
     2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
     2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
     2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
     2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
     2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
     2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
     3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
     3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
     3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc
     3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
     3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
     3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
     3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca
     3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
     3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
     3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
     3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
     3781 atcccactac cgagatgtcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
     3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
     4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga
     4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
     4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
     4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
     4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
     4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatcga tctcgatccc
     4981 gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa
     5041 ataattttgt ttaactttaa gaaggagata tacatatggg cagcagccac caccaccacc
     5101 accacagcag cggcctggtc ccgcgtggtt cccacatggc aggtccaccg ccgttcccgc
     5161 gtcgtcgtgg cccgagcggc cgtcgtcgtt gcggcggtcg tgctaccccg ggtagcgtcc
     5221 gtgaccgtac gggtcgtcgt ccggcagccg ttccgagccg tgcagtttgc gcagcggatc
     5281 tgtgcgaaga aaatgacgac ggctcgaaga atgtttctga acatcgcggt agcgcaggtg
     5341 gttccgtgct gtttccacgt accggcaccg tcctgccgtg ggtactgacc ggtcctggcg
     5401 cagcggcggt tcgcgcacgc tccgaagcac tgcgcacgca cctgcgtgcg agcaccgagt
     5461 ggtcccctgc gggcgtcggt caggcgctgc tggccggtac gggtgcgggt gccgataccc
     5521 accgtgccgt tgttctggca ggcgaccgtg cccagaccct gaacgcattg gcagcgctga
     5581 gcgcaggcgc agaccacccg gcagttttca ccagcactcg tgcggatgca agcccggctg
     5641 gcccggtgtt tgtgttcccg ggtcaaggct cgcagtggac cggtatggct cgtgaactgc
     5701 tggactccgc accggttttc gcgcgtaagc tgcacgactg tgcagacgcg tttgccccgt
     5761 acctgggcca cagcctgctg gatagcgtca ccggtgcagc aggtggtcca gagcctgttg
     5821 gcgcggacgt cgtccaaccg gcgctgttcg ccgttatggt tgcgctgact gatctgtgga
     5881 acgcggctgg cgttgcaccg ggtgcactgc tgggtcactc cctgggtgaa ctggcagccg
     5941 cgcatgtcgc gggtgttctg tccctggacg attctgctcg cgtcgtggcg cgttggagcc
     6001 aagcgcaggc tacgttggcg ggtcgtggtg acatggtcag cgttctgttg cctgcggatg
     6061 aattggcgga cctgctggac cgccgttggc cgggtcgctt ggttgtggcg gttgaaaacg
     6121 gtccaggtag cgcggtcgcg agcggtgacc tggacgctgc ggcggaactg gtcgcacacc
     6181 tgaccgccga aggtatccac gcgcgtcgcg ttgacgtggg cctggcggct cacagcccgc
     6241 acattgacgc gatcctgcca cgtattcgcg cggacatcgc gccgattcgt gcgcatacgc
     6301 cgagcatccc ggtttattcg gcgctgcatg gtggtgcact ggatggcacg ccaatggacg
     6361 cggcgtactg gtgtcgtaat ctgcgctcca ctgtacgttt cgcggacgcg acccgtgcag
     6421 ccctggaggc aggccatacc acgtttgtgg aggtaagccc acatccggtc ctgactacgg
     6481 cgatggaggt gagcgcaacc cgtgccgcgc acgcagcaac tgtcctgggt acgctgcgcc
     6541 gtggtgaggg tggtccgagc cgcttcctgg cgagcctggc cgaactgcat gtcagcggtg
     6601 gtgatgccga tctgcgtacg gttctgccgg cgagccaggc ggctggcttg ccggaaaccg
     6661 ttctgacggc gggtccgcgt ggcgagagcg cggatggcga ctctcgtcat gaggttctgt
     6721 gcgcacgcct ggcaccgctg gacccagcgg agcgtcgtgc ccagctgctg actgttgttc
     6781 gtgaaagcgc agctgccgcg ctggacggcg acgatcaagg tagcattgac ggtcgtcgca
     6841 cgttccgtga cctgggtatc acgtcgctgg cagcggtggg catccgtgat cgcctgcatt
     6901 ccgcaaccgg tctgcgtctg tctccgaccg ttgtgtttga tcatccgacc ccggacgcac
     6961 tggcggcaca cttggacacc gaactgttcg gcacgggcgc agatgccgag ccggcaccag
     7021 ctgcgggtgg tcgtgcggtg ccgcatgacg aaccaattgc gatcgtgggt atggcgtgcc
     7081 gttaccctgg cggcgttggt gcaccggccg acctgtggcg taccgttctg gccggtgtcg
     7141 acgcagttgg tccgctgccg gctgatcgtg gctggaatat tgcggacggt tacgatccgg
     7201 agctggcggg tcctggtcgt tttagccagc gtgagggcgg ctttctgcac gacgcagctg
     7261 aatttgatgc ggagttcttt ggtattagcc cgcgtgaggc attggcgatg gacccgcagc
     7321 aacgtttggc tctggaaagc gcctgggaag cgattgagga tgcgggtctg gacgcccata
     7381 gcctgcgtgg cagccgtact ggcgtttttc tgggcttgat tacccaggat tatggtcctc
     7441 gtgcgggtga gccgaccacg cgtgcaggtg cggtggaggg tcacctgttc ctgggtagca
     7501 ctggcagcgt cgcaagcggt cgtctgagct ataccttggg tctggaaggt ccgtctttga
     7561 cgattgatac ggcatgttcg agcagcctgg tggcactgca cgaagcatgt caagcgctgc
     7621 gtaccggtga ttgcgacatg gctctgactg gtggtgtgac ggtcatgccg agcaccggca
     7681 tgttggtcga gttcagccgt cagcgtggtc tgtcgcctga cggccgttgt aaagcctttt
     7741 ctgcatctgc cgacggtttt ggtctggcgg aaggtgtcgg tatgctggtg gttgagcgtc
     7801 tgagcgatgc gcgtcgtctg ggccatcgtg tgctggcggt ggtgcgcggt tctgcggtta
     7861 accaagatgg cgcgagcaat ggcctgtcgg cgcctagcgg cccagcacaa cagcgcgtta
     7921 ttcgccaggc gctggtcaac gctggcgtcc aagcatccca agtggacgtt gtcgaagcac
     7981 atggcaccgg tacgaaactg ggcgatccga ttgaggctca agccctgcaa gcgacctatg
     8041 gccagggccg tccggctgag cgcccgttgt ggttgggttc tctgaagtcc aatatcggcc
     8101 acgcgcaagc ggcagcgggt gtgggcggtg ttatcaaaat ggtcatggcg ttgcgtgaag
     8161 gcgtcctgcc accgaccctg cacgcagacg agccgagccc gcatattgac tggtcggcgg
     8221 gtcaggttcg tctgctgacc gaggaacgcg agtggccgga ggcaggtcac cctcgccgtg
     8281 cggcagtttc gagcttcggt gttagcggta ccaacgcgca cgtgatcatt gaagaggcgc
     8341 ctgaagaggg cgaagaaccg gagtctgacg ccggtggtgt tgtgccgtgg gttttgtctg
     8401 ctcgtacgga aggcgcgttg caggcccagg ctgttcagtt gagcgagttc gttggtgaga
     8461 gctcgccggt cgacgtgggt tggtccctgg tttctactcg tgcggccttc gagcatcgtg
     8521 cggtggtggt tggccgtggt cgcgacgagc tggtacgtgg tctgagcgag gtcgcgcagg
     8581 gtcgtggtgt tcgtggcgtg gcaagcagcg cgtccggtgg tctggctttc gttttcgcag
     8641 gccagggtag ccagcgcctg ggcatgggtc gcggcttgta tgagcgtttc ccggtgtttg
     8701 cagaagcgtt tgatgaggtg tgtggtcgcg ttggtccggg cgtacgcgag gttgtttttg
     8761 gtagcgacgc gggtgagctg gaccgtaccg tttgggcaca agccggcctg ttcgccctgg
     8821 aagtcgcgct gtttcgtctg ttggagagct ggggcgttcg tccgggttgc ctgattggcc
     8881 actccgtcgg cgagctgtcc gcggcatgcg tggcgggtct gtggagcctg gaggacgcgt
     8941 gccgcgttgt ggccgctcgt gcacgtctga tgcaggccct gccggctggc ggcgtcatgg
     9001 ttgctgttcg cgcggaagct ggcgagctgg ccggtttcct gggtgaggat gtggtgattg
     9061 caagcgtgaa cgcgccaggt caagtcgtga tcgcgggtcc ggaaggcggc gtcgagcgtg
     9121 tggttgctgc atgcggtgcc cgttcgcgcc gtctggcggt gagccatgcg ttccattccc
     9181 cgctggtgga gccgatgctg ggtgagtttc gccgtgtcgt tgagagcgtg gcatttggcg
     9241 tcccgagcct gcgtgtcgtc agcaacgtga ccggtgcgtg ggttgatccg gaagagtggg
     9301 gcaccccgga gtactgggtg cgtcaggtgc gcgaaccggt ccgttttgcg gatggtgtcg
     9361 cgacgttgct ggatgccggc gtccgtacct ttgttgagtt gggcccagcg ggtgcgctga
     9421 ccagcatggt tagccactgt gccgacgcga ctgcgacgtc tgtgaccgcc gtcccgacgc
     9481 tgcgtcctga ccacgacgaa agccgtacgg ttctgagcgc tgctgccagc ctgtatgtgc
     9541 agggccatcc ggttgattgg gcgcctctgt tcccacgcgc acgcaccgtt gatctgccga
     9601 cgtacgcatt tcagcatcag cgttactggc tggatagcac cactgcaaac actggtgacc
     9661 gtccggcagc ggaccgtgac accgcatttt gggaagctgt gcagcacacc gacctggacg
     9721 ccttcgctgc agaattggac attgccccgg atgcgccgtt gggcaccgtc ttgccggctc
     9781 tggctgactg gcgtcaacgc ctgcgtacgg cagcggctgt tgacgcatgg cgttaccgca
     9841 ccgcctttaa acgtctgcca gatgcgccag gtgcaccagt cctgacgggc agctggctgg
     9901 ccgtagttcc ggtgcgtcac ctggatgatc cgagcgttac cactagcctg gatgcagttg
     9961 ctaaagcggg tgcggaagtc gttcagttgg caatcgaaga tgcggacgcg gacgttgatc
    10021 gtctgactga gcgcttgcgt ggcctggttg ccggtctggg tgccgcgccg gcgggcatta
    10081 tgagcttcct gggtctggat gaagagcgtc atcgtgacca cccggcgatg ccgagcggtc
    10141 tggccaccag cttggcgctg gtccgcgcct tgggtcgtgc gggcatcggt gcaccgctgt
    10201 ggatggttac gcgtgaggca gtggcagcgg gtcaagacac gcacccgcat gcgcctctgg
    10261 gtagcctgat ctggggtctg ggccaagtga cggctctgga gcacgcagat cgctggggtg
    10321 gtctgatcga tctgccgggt gtgtgtgatg cgcgcgttgc ccgcatgctg tgcgcgggtc
    10381 tgagcggccg tggtgccgaa gatcagctgg ccctgcgtcc gagcggcact ttcgtccgcc
    10441 gtctggcgca tatccctggc gagcaacgtg cagcacgtcg tagctggcaa ccacgtggta
    10501 cggtgattgt caccggtggt acgggtgcgc tgggtgcagt cctggcccgc tggttggcta
    10561 ccgaggacgc ggagcacctg gtgctgaccg gccgtcgtgg cgcggacgcc cctggcgcgg
    10621 agcgtttgcg tgacgagctg gtcgctacgg gcgctcgtgt cacgctggcg gcgtgcgacg
    10681 tggcagatcg caaagccgtc gccgcattgc tggacgaact ggctgcggac ggcgagactg
    10741 ttcgcgcagt tctgcacgct gcgggtgtcg ccgatctgac gtcgctggag aataccggtc
    10801 cagaagcgtt cgcggcaggc gtggccgcga aggtcgatgg tgcactgcac ctgaccgaac
    10861 tgttggatca cgattcgctg gatgcgtttg tgttgttcag cagcattgcg ggtgtttggg
    10921 gttccggcga ccacggcgcg tatgcggctg cgaacgcatt tctgaatgcg ttggcagagt
    10981 acaatcgtgc acgcggtatc ccgaccacga gcatcgcatg gggcgtttgg aacgcgtttg
    11041 gcgtcgaggg tgcaggcggt atcagcgagg cggttgattt ggaccagctg catcgtcgcg
    11101 gcctgccgct gattgaacca gagctgggtc tgactgcact gcgtcgcgct ctagaccgtg
    11161 acgaaacggt gctgacggtt gctccggttg cctgggagcg cttctttccg ctgttctccg
    11221 ctgcacgtcc gcgtccgttg tttgaggact tgccgcaagt gcgtgccctg agcgcacctg
    11281 tcccgacgac ggcgggtccg gccgtggaac caggtcgccg tggtagcggc ctgggcgatt
    11341 tgcctctgac ggatcgcgat agcgcgctgc tggccttggt ccgcggtgag agcgcatccg
    11401 tgctgggtta cgagcgtcca gatcgcctgg acccggaccg tgcgctgcgt gatgtgggtt
    11461 tcgatagcct gacggcgatg gaactgcgta accgtctggc taccgcgacc ggcctgacgc
    11521 tgcctgcggc cctggtgttt gatcacccga ccccactggc gatcgcggcg tatctgaaag
    11581 ccgagctgac gagccagctg gactccggta cgcctgcacg tgaagctagc tctgcactgc
    11641 gtgacggcta ccgccaagcg ggtgtgagcg gccgtgttcg tagctacctg gatctgttgg
    11701 caggtctgtc ggatttccgc gaacattttg atggtagcga tggttttagc ctggatctgg
    11761 ttgatatggc agatggtccg ggtgaggtga ccgttatttg ctgcgcgggt acggctgcga
    11821 tctctggtcc gcatgagttc acccgtctgg caggtgccct gcgcggcatt gcacctgttc
    11881 gtgcggtgcc gcagccgggt tacgaagaag gcgaaccgct gccttctagc atggcggctg
    11941 ttgcagctgt gcaggctgat gctgtcattc gtacgcaagg cgataagccg ttcgtggttg
    12001 cgggtcactc ggcaggcgcg ctgatggcgt acgcgctggc gaccgaactg ctggatcgtg
    12061 gtcacccgcc tcgcggtgtt gtcctgattg atgtgtaccc gcctggtcat caggacgcga
    12121 tgaacgcgtg gctggaggaa ctgacggcaa cgttgttcga ccgtgaaact gttcgtatgg
    12181 acgacacccg tctgaccgcg ttgggtgctt acgaccgcct gacgggtcaa tggcgtcctc
    12241 gcgaaaccgg tttgccgacc ctgttggtta gcgcgggtga accaatgggc ccgtggccgg
    12301 acgatagctg gaaaccgacc tggcctttcg agcacgacac cgttgcggtc ccgggtgatc
    12361 atttcacgat ggttcaagaa catgctgatg cgattgcccg tcacattgac gcctggctgg
    12421 gtggcggcaa cagctgataa gcttatagga tccgaattcg agctccgtcg acaagcttgc
    12481 ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa
    12541 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
    12601 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
//
