LOCUS       sequence-20708-e         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG425GPD-ccdB-HA
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(41..165)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(173..189)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(173..189)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(195..458)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(278..301)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 agcataaagt gtaaagcctg ggggtgccta atgcggccgc catagtgact ggatatgttg
       61 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
      121 tatatcattt tacgtttctc gttcagcttt tttgtacaaa cttgtgatgg gggatccact
      181 agttctagaa tccgtcgaaa ctaagttctg gtgttttaaa actaaaaaaa agactaacta
      241 taaaagtaga atttaagaag tttaagaaat agatttacag aattacaatc aatacctacc
      301 gtctttatat acttattagt caagtagggg aataatttca gggaactggt ttcaaccttt
      361 tttttcagct ttttccaaat cagagagagc agaaggtaat agaaggtgta agaaaatgag
      421 atagatacat gcgtgggtca attgccttgt gtcatcattt actccaggca ggttgcatca
      481 ctccattgag gttgtgcccg ttttttgcct gtttgtgccc ctgttctctg tagttgcgct
      541 aagagaatgg acctatgaac tgatggttgg tgaagaaaac aatattttgg tgctgggatt
      601 cttttttttt ctggatgcca gcttaaaaag cgggctccat tatatttagt ggatgccagg
      661 aataaactgt tcacccagac acctacgatg ttatatatt
//
