LOCUS       sequence-20709-e         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG425GPD-ccdB-HA
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(120..244)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             285..374
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      393..640
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(409..427)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(658..677)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(659..677)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
       61 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc
      121 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct
      181 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag
      241 tggtgatggg ctgcaggaat tcgatatcaa gcttaggtgg aatgtaccca tacgatgttc
      301 ctgactatgc gggctatccc tatgacgtcc cggactatgc aggatcctat ccatatgacg
      361 ttccagatta cgctgctcag tgctaactcg agtcatgtaa ttagttatgt cacgcttaca
      421 ttcacgccct ccccccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt
      481 ctaggtccct atttattttt ttatagttat gttagtatta agaacgttat ttatatttca
      541 aatttttctt ttttttctgt acagacgcgt gtacgcatgt aacattatac tgaaaacctt
      601 gcttgagaag gttttgggac gctcgaaggc tttaatttgc ggccggtacc caattcgccc
      661 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
//
