LOCUS       sequence-207092-        6735 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High copy number plasmid containing iCreERT2 synthesized fragment 
            for constructing the HPRT plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6735)
  AUTHORS   Korecki AJ, Hickmott JW, Lam SL, Dreolini L, Mathelier A, Baker O, 
            Kuehne C, Bonaguro RJ, Smith J, Tan CV, Zhou M, Goldowitz D, 
            Deussing JM, Stewart AF, Wasserman WW, Holt RA, Simpson EM
  TITLE     Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye 
            and Brain; Including Seventeen Carrying a New Inducible-First 
            Constitutive-Ready Allele.
  JOURNAL   Genetics. 2019 Feb 14. pii: genetics.119.301984. doi: 
            10.1534/genetics.119.301984.
  PUBMED    30765420
REFERENCE   2  (bases 1 to 6735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2019 Feb 14. pii: genetics.119.301984. doi: 
            10.1534/genetics.119.301984."
COMMENT     Sequence Label: pEMS1925
FEATURES             Location/Qualifiers
     source          1..6735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(496..513)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(496..512)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(611..628)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(782..1370)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(862..881)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1492..2283,2284..2352))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2115..2134
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2353..2444)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2486..2505
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2511..2530
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     polyA_signal    3305..3426
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3342..3361)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3396..3415
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             3547..3558
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3956..3976
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    complement(4046..4079)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     CDS             complement(4102..4995)
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     protein_bind    complement(5021..5054)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(6109..6128)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          6111..6243
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 ggtctcctat acgtctctta tacgacatca ccgatgggga acccagacgc tgagtacgta
       61 ttctaaatgc ataataaata ctgataacat cttatagttt gtattatatt ttgtattatc
      121 gttgacatgt ataattttga tatcaaaaac tgattttccc tttattattt tcgagattta
      181 ttttcttaat tctctttaac aaactagaaa tattgtatat acaaaaaatc ataaataata
      241 gatgaatagt ttaattatag gtgttcatca atcgaaaaag caacgtatct tatttaaagt
      301 gcgttgcttt tttctcattt ataaggttaa ataattctca tatatcaagc aaagtgacag
      361 gcgcccttaa atattctgac aaatgctctt tccctaaact ccccccataa aaaaacccgc
      421 cgaagcgggt ttttacgtta tttgcggatt aacgattact cgttatcaga accgcccagg
      481 gggcccgagc ttaagactgg ccgtcgtttt acaacacaga aagagtttgt agaaacgcaa
      541 aaaggccatc cgtcaggggc cttctgctta gtttgatgcc tggcagttcc ctactctcgc
      601 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
      661 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      721 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      781 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      841 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      901 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      961 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1021 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1081 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1141 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggg
     1201 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     1261 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1321 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     1381 tgatcttttc tacggggtct gacgctcagt ggaacgacgc gcgcgtaact cacgttaagg
     1441 gattttggtc atgagcttgc gccgtcccgt caagtcagcg taatgctctg cttaccaatg
     1501 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     1561 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagcgctgc
     1621 gatgataccg cgagaaccac gctcaccggc tccggattta tcagcaataa accagccagc
     1681 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     1741 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     1801 catcgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     1861 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     1921 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     1981 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     2041 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     2101 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     2161 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     2221 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     2281 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     2341 ttgaatactc atattcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     2401 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg tcagtgttac
     2461 aaccaattaa ccaattctga acattatcgc gagcccattt atacctgaat atggctcata
     2521 acaccccttg tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact
     2581 cagaagtgaa acgccgtagc gccgatggta gtgtggggac tccccatgcg agagtaggga
     2641 actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgcccgggc
     2701 taataagggg ggctggatcg cttcgtgttc cccatcggtg atgtcgtata ggaagcagta
     2761 tacgagacct ataggagacg tatatggtct tcttttgtcg actttttgaa ttcggcattc
     2821 aaggccaggc tggatgtggc tctgggcagc ctgggctgct ggttgatgac cctgcacata
     2881 gcagggggtt ggatctggat gagcactgtg ctcctttgca acccaggccg ttctatgatt
     2941 ctgtcattct aaatctctct ttcagcctaa agctttttcc ccgtatcccc ccaggtgtct
     3001 gcaggctcaa agagcagcga gaagcgttca gaggaaagcg atcccgtgcc accttccccg
     3061 tgcccgggct gtccccgcac gctgccggct cggggatgcg gggggagcgc cggaccggag
     3121 cggagccccg ggcggctcgc tgctgccccc tagcggggga gggacgtaat tacatccctg
     3181 ggggctttgg gggggggctg tccccgtgag ctctaccaca tttgtagagg ttttacttgc
     3241 tttaaaaaac ctcccacatc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     3301 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     3361 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     3421 atcttatcat gtctgcgatc gcggggaggc ggcccaaagg gagatccgac tcgtctgagg
     3481 gcgaaggcga agacgcggaa gaggccgcag agccggcagc aggccgcggg aaggaaggtc
     3541 cgctggattg agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc
     3601 aacacaggcg agcagccaag gaaaggacga tgatttcccc gacaacacca cggaattgtc
     3661 agtgcccaac agccgagccc ctgtccagca gcgggcaagg caggcggcga tgagttccgc
     3721 cgtggcaata gggaggggga aagcgaaagt cccggaaagg agctgacagg tggtggcaat
     3781 gccccaacca gtgggggttg cgtcagcaaa cacagtgcac accacgccac gttgcctgac
     3841 aacgggccac aactcctcat aaagagacag caaccaggat ttatacaagg aggagaaaat
     3901 gaaagccata cgggaagcaa tagcatgata caaaggcatt aaagcagcgt atccacatag
     3961 cgtaaaagga gcaacatagt taagaatacc agtcaatctt tcacaaattt tgtaatccag
     4021 aggttgagcg atcgcggccg ctcaagaagt tcctatacta tttgaagaat aggaacttcc
     4081 gccagggagc tcagatcttc atcaagctgt ggcagggaaa ccctctgcct cccccgtgat
     4141 gtaatacttt tgcaaggaat gcgatgaagt agagcccgca gtggccaagt ggctttggtc
     4201 cgtctcctcc acggatgccc ctccacggct agtgggcgca tgtaggcggt gggcgtccgc
     4261 cgcctccagc agcaggtcat agaggggcac cacgttcttg cacttcatgc tgtacagatg
     4321 ctccatgcct ttgttactca tgtgcctgat gtgggagagg atgaggagga gctgggccag
     4381 ccgctggtgc tgctgctgca gggtcaggcc tgccttggcc atcaggtgga tcaaagtgtc
     4441 tgtgatcttg tccaggactc ggtggatatg gtccttctct tccagagact tcagggtgct
     4501 ggacagaaat gtgtacactc cagaattaag caaaataata gatttgaggc acacaaactc
     4561 ctctccctgc agattcatca tgcggaaccg agatgatgta gccagcagca tgtcgaagat
     4621 ctccaccatg ccctctacac attttccctg gttcctgtcc aagagcaagt taggagcaaa
     4681 cagtagcttc actgggtgct ccatggagcg ccagacgaga ccaatcatca ggatctctag
     4741 ccaggcacat tctagaaggt ggacctgatc atggagggtc aaatccacaa agcctggcac
     4801 cctcttcgcc cagttgatca tgtgaaccag ctccctgtct gccaggttgg tcagtaagcc
     4861 catcatcgaa gcttcactga agggtctggt aggatcatac tcggaataga gtatgggggg
     4921 ctcagcatcc aacaaggcac tgaccatctg gtcggccgtc agggacaagg ccaggctgtt
     4981 cttcttagag cgtttgatca tgagcgggct tggccaaagt gaagttccta tactatttga
     5041 agaataggaa cttccgtccc catcctcgag cagcctcacc atggccccag tctcagagtc
     5101 caggtttctg atgtagttca tcactatgtt cacattggtc cagccaccag cctgcatgat
     5161 ttcagggatg gacacaccag ccctggccat gtccctggca gcacccactc tggcagagtg
     5221 gccagaccag gccaggtatc tctgcccaga gtcatccttg gcaccataga tcaggcggtg
     5281 ggtggcctca aagatccctt ccagggcccg ggtggacagt tgggaggtgg cagaaggggc
     5341 agccacacca ttctttctga cccggcagaa caggtagttg ttggggtcat cagccacacc
     5401 agacacagag atccatctct ccaccagctt ggtaaccccc agggacaggg ccttctccac
     5461 accagctgtg gacaccaggg tcttggtcct gccaatgtgg atcagcattc tcccaccatc
     5521 ggtgcgggag atgtccttca ctctgattct ggcaatttcg gcaatgcgca gcagggtgtt
     5581 gtaggcaatg cccaggaagg ccaggttcct gatgtcctgg catctgtcag agttctccat
     5641 cagggatctg acttggtcaa agtcagtgcg ttcaaaggcc agggcctgct tggctctctc
     5701 cccagcatcc acattctcct ttctgattct cctcatcacc agggacacag cattggagtc
     5761 agaagggcga ggcaggccag atctcctgtg cagcatgttg agctggccca ggtgctgttg
     5821 gatggtcttc acagccaggc ctctggcttg caggtacagg aggtagtccc tcacatcctc
     5881 aggttcagca gggaaccatt tcctgttgtt cagcttgcac caggcagccc aggatctgca
     5941 cacagacagg agcatcttcc aggtgtgttc agagaaggcc tgcctgtccc tgaacatgtc
     6001 catcaggttc ttcctgactt catcagaggt ggcatccaca gggagggcag gcaggttttg
     6061 gtgcacagtc agcaggttgg acatggtggc ggccgcctgg gagtggacac ctgtggagag
     6121 aaaggcaaag tggatgtcag taagaccaat aggtgcctat cagaaacgca agagtcttct
     6181 ctgtctcgac aagcccagtt tctattggtc tccttaaacc tgtcttgtaa ccttgatact
     6241 tacctgccca gtgcctcacg accaacttcg gcgcgccacg cgtggccggc ctaggggcat
     6301 tcaaggccag gctggatgtg gctctgggca gcctgggctg ctggttgatg accctgcaca
     6361 tagcaggggg ttggatctgg atgagcactg tgctcctttg caacccaggc cgttctatga
     6421 ttctgtcatt ctaaatctct ctttcagcct aaagcttttt ccccgtatcc ccccaggtgt
     6481 ctgcaggctc aaagagcagc gagaagcgtt cagaggaaag cgatcccgtg ccaccttccc
     6541 cgtgcccggg ctgtccccgc acgctgccgg ctcggggatg cggggggagc gccggaccgg
     6601 agcggagccc cgggcggctc gctgctgccc cctagcgggg gagggacgta attacatccc
     6661 tgggggcttt gggggggggc tgtccccgtg agctcggatc cattaccctg ttatccctac
     6721 cccgaagaca ctata
//
