LOCUS       sequence-20712-e         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG426GAL-ccdB-HA
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(126..250)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             291..380
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      399..646
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(415..433)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(664..683)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(665..683)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgttatcggg ggagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat
       61 taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg
      121 tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca
      181 aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt
      241 acaaagtggt gatgggctgc aggaattcga tatcaagctt aggtggaatg tacccatacg
      301 atgttcctga ctatgcgggc tatccctatg acgtcccgga ctatgcagga tcctatccat
      361 atgacgttcc agattacgct gctcagtgct aactcgagtc atgtaattag ttatgtcacg
      421 cttacattca cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc
      481 tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat
      541 atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa
      601 aaccttgctt gagaaggttt tgggacgctc gaaggcttta atttgcggcc ggtacccaat
      661 tcgccctata gtgagtcgta ttacgcgcgc tca
//
