LOCUS       sequence-207142-        2673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Suicide vector based on the TetR/Pip OFF system. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Arnold FM, Hohl M, Remm S, Koliwer-Brandl H, Adenau S, Chusri S, 
            Sander P, Hilbi H, Seeger MA
  TITLE     A uniform cloning platform for mycobacterial genetics and protein 
            production.
  JOURNAL   Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.1038/s41598-018-27687-5.
  PUBMED    29934571
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2018-06-22"; volume: "8(1):9539. doi"; pages: " 
            10.1038/s41598-018-27687-5"
COMMENT     Sequence Label: pCOND (Hyg)
FEATURES             Location/Qualifiers
     source          1..2673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(387..404)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(558..1146)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(638..657)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaaaggccct ggcatcctcg atcatctcct ccagggtggt cggccgagct tccatcccag
       61 ctcggcaagg atcaacgagg acagtcgcac gacgaagttc ttctggatcg cgcccgtgct
      121 ggaagcactc aacctcgaag cgtgtggttg cggagccatc tagcaaccac acgaggatcc
      181 agatctccat cctgacggat ggccttacga gttccgagat caccccggtc acggtggacg
      241 gcgtcacctt ctacgacctg accgcggaga aatagcgctg tacagcgtat gggaatctct
      301 tgtacggtgt acgagtatct tcccgtacac cgtacaagga gcctgaatga gttgaagagc
      361 ggatccgctc ttcagcattc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      421 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      481 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      541 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      601 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      661 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      721 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
      781 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
      841 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
      901 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
      961 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     1021 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1081 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     1141 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     1201 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     1261 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     1321 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     1381 gcctgactcc ccggacgtgg ccgaccagcc cgtcatcgtc aacgcctgac cgcggtgcgg
     1441 acaggccgtg tcgcgaccgg ccgtgcggaa ttaagccggc ccgtaccctg tgaatagagg
     1501 tccgctgtga cacaagaatc cctgttactt ctcgaccgta ttgattcgga tgattcctac
     1561 gcgagcctgc ggaacgacca ggaattctgg gagccgctgg cccgccgagc cctggaggag
     1621 ctcgggctgc cggtgccgcc ggtgctgcgg gtgcccggcg agagcaccaa ccccgtactg
     1681 gtcggcgagc ccgacccggt gatcaagctg ttcggcgagc actggtgcgg tccggagagc
     1741 ctcgcgtcgg agtcggaggc gtacgcggtc ctggcggacg ccccggtgcc ggtgccccgc
     1801 ctcctcggcc gcggcgagct gcggcccggc accggagcct ggccgtggcc ctacctggtg
     1861 atgagccgga tgaccggcac cacctggcgg tccgcgatgg acggcacgac cgaccggaac
     1921 gcgctgctcg ccctggcccg cgaactcggc cgggtgctcg gccggctgca cagggtgccg
     1981 ctgaccggga acaccgtgct caccccccat tccgaggtct tcccggaact gctgcgggaa
     2041 cgccgcgcgg cgaccgtcga ggaccaccgc gggtggggct acctctcgcc ccggctgctg
     2101 gaccgcctgg aggactggct gccggacgtg gacacgctgc tggccggccg cgaaccccgg
     2161 ttcgtccacg gcgacctgca cgggaccaac atcttcgtgg acctggccgc gaccgaggtc
     2221 accgggatcg tcgacttcac cgacgtctat gcgggagact cccgctacag cctggtgcaa
     2281 ctgcatctca acgccttccg gggcgaccgc gagatcctgg ccgcgctgct cgacggggcg
     2341 cagtggaagc ggaccgagga cttcgcccgc gaactgctcg ccttcacctt cctgcacgac
     2401 ttcgaggtgt tcgaggagac cccgctggat ctctccggct tcaccgatcc ggaggaactg
     2461 gcgcagttcc tctgggggcc gccggacacc gcccccggcg cctgacgccc cgggccgccc
     2521 ggcgccgccc ccggcccccg gcggccggcc gcccggagcc ccgcccgcgc tcgggagccc
     2581 cgggcccgcg ccgaagcccg ctgctgcgag cccggagcgg gccggccgac ggcggtgcgg
     2641 gcccggcggc ggacgctcag cagcggcggg cgt
//
