LOCUS       sequence-207173-        8514 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Flag-tagged human UAP56 (DDX39B).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Huang C, Liang D, Tatomer DC, Wilusz JE
  TITLE     A length-dependent evolutionarily conserved pathway controls nuclear
            export of circular RNAs.
  JOURNAL   Genes Dev. 2018 May 17. pii: gad.314856.118. doi: 
            10.1101/gad.314856.118.
  PUBMED    29773557
REFERENCE   2  (bases 1 to 8514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2018 May 17. pii: gad.314856.118. doi: 10.1101/gad.314856.118."
COMMENT     Sequence Label: Hy_pMT Flag-UAP56 WT
FEATURES             Location/Qualifiers
     source          1..8514
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     621..640
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(859..963)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1031..1049
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2445..2724
                     /label=copia promoter
                     /note="strong promoter from the Drosophila transposable 
                     element copia (Sinclair et al., 1986)"
     CDS             2770..3795
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2770..3795
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     intron          4158..4223
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4353..4373
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4798..4932
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4933..5359
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     CDS             5378..5443
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    6751..6872
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7582..7599)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7753..8341)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7833..7852)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8512..8514,1..789,790..858))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
       61 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
      121 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
      181 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
      241 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
      301 tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
      361 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
      421 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
      481 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
      541 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
      601 ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat
      661 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
      721 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
      781 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
      841 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
      901 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
      961 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     1021 aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattca catttgtacg
     1081 aatttttttt ttatcaaaag ttcgagtttt tcaccaattt cctcatcaac cgagcaaggc
     1141 aaacggcttt gaataatatg gtgttatata tacatatatc aaatcgctgc tgactgcgtg
     1201 attgatggcc ccaagattac atattatcga atcaggattc agaaggagat caatgtcaaa
     1261 tgcggacagg aacatgaaag acgcctgtta tgcgcaatta aaaatttggg tttaattgct
     1321 gtggaaactg ttgttggcgg catcttaagt tcctgtttaa caacatcaac tacttatgta
     1381 cgtagaagcg tttaagccat ttgcatacag atgagaactg gcttttgtgc taatcagtca
     1441 agatgactcc gatgatgatg actcattacc tgaccagttt tcgctgcttt cttttcaaca
     1501 actacttgta tatgtattgt atccaatagc aatacattga atttccatgg tctagtcacg
     1561 tattatcatt taattgacac caagtcgtgt tattgttgag ctatcgagtt cagctcaaac
     1621 atttcttatt cccatgaata agccggcaaa aatatgcaat ctatgaaagt taatataagc
     1681 aaaccttact ttgactcaat accaatgcac tttgtgtcga taggttcacg caattgaggc
     1741 gattattccg ataacccaag cgattgactg ttcccgtttc gattccaatt gaaatttgga
     1801 aatgtacaat agttttgcta tatgctgtca agtacgctct tatcttctct gggttttctt
     1861 cagagtttcg aaacgcttct tctttttttt gttttttttt ttttggaatc tcgtattttg
     1921 gaaggggctc ccctctggaa tttgttacac tgtcgttatc attgcgaaca agcggcccga
     1981 agctatcagc gactttaaca tttacaatgc acttttttac gaccaattaa atgtacattt
     2041 tcctttcttc gcccgttgat aagcgaacgc gatgtggcgc aggcaatgtg ttgctcttgc
     2101 gacacaaacg caatcaaaat ggattcaatt tcgctttttc ccagtgaaac gaagaacgaa
     2161 ccgaccatca tgatatgctc ctctgcatgt tgcgtattga atcaatgaca atttcaatta
     2221 agccgcccgt tcgtcatgcg ttttcgtgcg cttcgaaatg ctgataacgc tgctgtcctc
     2281 caactgcttt gcatgtggac acaattccat ttatttaatt cttttatttg gatcggttaa
     2341 attaaaaagc gccttgttac gcatttaacg ttgtttccgg tgcgtggtgg tttcatgctt
     2401 ctgggaacgg caaatgggtt taggattggg aacccctcat catctgttgg aatatactat
     2461 tcaacctaca aaagtaacgt taaacaacac tactttatat ttgatatgaa tggccacacc
     2521 ttttatgcca taaaacatat tgtaagagaa taccactctt tttattcctt ctttccttct
     2581 tgtacgtttt ttgctgtaag taggtcgtgg tgctggtgtt gcagttgaaa taacttaaaa
     2641 tataaatcat aaaactcaaa cataaacttg actatttatt tatttattaa gaaaggaaat
     2701 ataaattata aattacaaca ggttatggac ctgcagccaa gcttggcgct cgtccggggg
     2761 caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
     2821 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
     2881 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
     2941 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
     3001 cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt
     3061 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag
     3121 gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
     3181 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
     3241 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct
     3301 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
     3361 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
     3421 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
     3481 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
     3541 ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
     3601 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
     3661 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg
     3721 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
     3781 agggcaaagg aatagagtag atgccgaccg aacaagagct gatttcgaga acgcctcagc
     3841 cagcaactcg cgcgagccta gcaaggcaaa tgcgagagaa cggccttacg cttggtggca
     3901 cagttctcgt ccacagttcg ctaagctcgc tcggctgggt cgcgggaggg ccggtcgcag
     3961 tgattcaggc ccttctggat tgtgttggtc cccagggcac gattgtcatg cccacgcact
     4021 cgggtgatct gactgatccc gcagattgga gatcgccgcc cgtgcctgcc gattgggtgc
     4081 agatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
     4141 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga
     4201 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt
     4261 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg
     4321 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc
     4381 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa
     4441 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa
     4501 ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat cataacatac
     4561 tgttttttct tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat
     4621 tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg
     4681 ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     4741 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     4801 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     4861 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     4921 catgtctgga tcaattcgtt gcaggacagg atgtggtgcc cgatgtgact agctctttgc
     4981 tgcaggccgt cctatcctct ggttccgata agagacccag aactccggcc ccccaccgcc
     5041 caccgccacc cccatacata tgtggtacgc aagtaagagt gcctgcgcat gccccatgtg
     5101 ccccaccaag agctttgcat cccatacaag tccccaaagt ggagaaccga accaattctt
     5161 cgcgggcaga acaaaagctt ctgcacacgt ctccactcga atttggagcc ggccggcgtg
     5221 tgcaaaagag gtgaatcgaa cgaaagaccc gtgtgtaaag ccgcgtttcc aaaatgtata
     5281 aaaccgagag catctggcca atgtgcatca gttgtggtca gcagcaaaat caagtgaatc
     5341 atctcagtgc aactaaaggg gggatctcga gaccatggac tacaaagacc atgacggtga
     5401 ttataaagat catgacatcg attacaagga tgacgatgac aagaccggtg gtaccatggc
     5461 agagaacgat gtggacaatg agctcttgga ctatgaagat gatgaggtgg agacagcagc
     5521 tgggggagat ggggctgagg cccctgccaa gaaggatgtc aagggctcct atgtctccat
     5581 ccacagctct ggctttcgtg acttcctgct caagccagag ttgctccggg ccattgtcga
     5641 ctgtggcttt gagcatccgt cagaagtcca gcatgagtgc atccctcagg ccattctggg
     5701 aatggatgtc ctgtgccagg ccaagtcggg catgggaaag acagcagtgt ttgtcttggc
     5761 cacactgcaa cagctggagc cagttactgg gcaggtgtct gtactggtga tgtgtcacac
     5821 tcgggagttg gcttttcaga tcagcaagga atatgagcgc ttctctaaat acatgcccaa
     5881 tgtcaaggtt gctgtttttt ttggtggtct gtctatcaag aaggatgaag aggtgctgaa
     5941 gaagaactgc ccgcatatcg tcgtggggac tccaggccgt atcctagccc tggctcgaaa
     6001 taagagcctc aacctcaaac acattaaaca ctttattttg gatgaatgtg ataagatgct
     6061 tgaacagctc gacatgcgtc gggatgtcca ggaaattttt cgcatgaccc cccacgagaa
     6121 gcaggtcatg atgttcagtg ctaccttgag caaagagatc cgtccagtct gccgcaagtt
     6181 catgcaagat ccaatggaga tcttcgtgga tgatgagacg aagttgacgc tgcatgggtt
     6241 gcagcagtac tacgtgaaac tgaaggacaa cgagaagaac cggaagctct ttgaccttct
     6301 ggatgtcctt gagttcaacc aggtggtgat ctttgtgaag tctgtgcagc ggtgcattgc
     6361 cttggcccag ctactagtgg agcagaactt cccagccatt gccatccacc gtgggatgcc
     6421 ccaggaggag aggctttctc ggtatcagca gtttaaagat tttcaacgac gaattcttgt
     6481 ggctaccaac ctatttggcc gaggcatgga catcgagcgg gtgaacattg cttttaatta
     6541 tgacatgcct gaggattctg acacctacct gcatcgggtg gccagagcag gccggtttgg
     6601 caccaagggc ttggctatca catttgtgtc cgatgagaat gatgccaaga tcctcaatga
     6661 tgtgcaggat cgctttgagg tcaatattag tgagctgcct gatgagatag acatctcctc
     6721 ctacattgaa cagacacggt aggcggccgc aacttgttta ttgcagctta taatggttac
     6781 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6841 tgtggtttgt ccaaactcat caatgtatct taactagtgg atccactagg ggccgccgac
     6901 gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc
     6961 cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg
     7021 atcgctcgcg gctcttacca gcctaacttc gatcattgga ccgctgatcg tcacggcgat
     7081 ttatgccgcc tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata
     7141 ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat
     7201 ggaagccggc ggcacctcgc taacggattc accactccaa gaattggagc caatcaattc
     7261 ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga acatatccat cgcgtccgcc
     7321 atctccagca gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc acgggtgcgc
     7381 atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct tactggttag
     7441 cagaatgaat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg
     7501 acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg
     7561 aagtcagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7621 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7681 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7741 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7801 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7861 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7921 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7981 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8041 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8101 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8161 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     8221 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8281 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8341 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8401 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8461 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gtta
//
