LOCUS       sequence-20718-e         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG303GAL-ccdB-TAP
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(100..224)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             283..360
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             388..408
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
       61 tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt
      121 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
      181 atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggtgatggg ctgcaggaat
      241 tcgatatcaa gcttatcgat aggtggacca ggtggtggaa tgaagcgacg atggaaaaag
      301 aatttcatag ccgtctcagc agccaaccgc tttaagaaaa tctcatcctc cggggcactt
      361 gattatggta ccccaactac tgcttctgaa aatctatatt ttcaaggtga actaaaaact
      421 gctgctttgg ctcaacatgc gattaagggt gaagctcaaa aacttaatga ctctcaagct
      481 ccaaaagctg atgcgcaaca aaataacttc aacaaagatc aacaaagcgc cttctatgaa
      541 atcttgaaca tgcctaactt aaacgaagcg caacgtaacg gcttcattca aagtcttaaa
      601 gacgacccaa gccaaagcac taacgtttta ggtgaagcta aaaaattaaa cgaatctcaa
      661 gcaccgaaag ct
//
