LOCUS       sequence-207185-        2940 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Shechner DM, Grant RA, Bagby SC, Koldobskaya Y, Piccirilli JA, 
            Bartel DP
  TITLE     Crystal structure of the catalytic core of an RNA-polymerase 
            ribozyme.
  JOURNAL   Science. 2009 Nov 27. 326(5957):1271-5.
  PUBMED    19965478
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2009 Nov 27. 326(5957):1271-5."
COMMENT     Sequence Label: p307HU
FEATURES             Location/Qualifiers
     source          1..2940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     155..172
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    289..310
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(325..330,331..348,349..355)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    363..379
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     368..390
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     387..403
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     387..403
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    416..472
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        476..494
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(727..744)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(727..743)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(736..758)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(952..971)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1071..1093
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1131..1149)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1217..1321
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1322..1390,1391..2182)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1540..1559)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(2353..2940,1)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2842..2861
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
       61 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      121 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      181 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
      241 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
      301 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
      361 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct
      421 tgcatgcctg caggtcgact ctagaggatc cccgggtacc gagctcgaat tcgcgtaata
      481 cgactcacta taggaacact atactactgg ataatcaaag acaaatctgc ccgaagggct
      541 tgagaacata cccattgcac tccgggtatg cagaggtggc agcctccggt gggttaaaac
      601 ccaacgttct caacaatagt gaggccggca tggtcccagc ctcctcgctg gcgccggctg
      661 ggcaacattc cgaggggacc gtcccctcgg taatggcgaa tgggacccac aaatgaagag
      721 ggattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
      781 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
      841 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
      901 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
      961 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     1021 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     1081 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     1141 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     1201 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     1261 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     1321 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     1381 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     1441 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     1501 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     1561 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     1621 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     1681 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     1741 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     1801 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     1861 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     1921 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     1981 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     2041 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     2101 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     2161 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     2221 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     2281 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     2341 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2401 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2461 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     2521 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2581 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2641 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2701 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2761 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2821 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2881 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
//
