LOCUS       sequence-207189-        6042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use together with pCMV-intron and VSV-G to package MMLV reporter 
            virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   Ooi SK, Wolf D, Hartung O, Agarwal S, Daley GQ, Goff SP, Bestor TH
  TITLE     Dynamic instability of genomic methylation patterns in pluripotent 
            stem cells.
  JOURNAL   Epigenetics Chromatin. 2010 Sep 24;3(1):17. doi: 
            10.1186/1756-8935-3-17.
  PUBMED    20868487
REFERENCE   2  (bases 1 to 6042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Epigenetics
            Chromatin."; date: "2010-09-24"; volume: "3(1):17. doi"; pages: " 
            10.1186/1756-8935-3-17"
COMMENT     Sequence Label: PNCA-GFP
FEATURES             Location/Qualifiers
     source          1..6042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    459..816
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             879..1472
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(1654..1672)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1740..1844
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1845..1913,1914..2705)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2063..2082)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2876..3464
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3365..3384
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3618..3635
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             3702..4171
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(join(4974..5687,5688..5690,5691..5693))
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5019..5040)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg
       61 cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt
      121 cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cgggcgaagc
      181 actgcacgcc gtaggtcaag gtggtcacga gggtgggcca gggcacgggc agcttgccgg
      241 tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg
      301 acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc
      361 cggtgaacag ctcctcgccc ttgctcacca tggatccatg catattctca gacaaataca
      421 gaaacacagt cagacagaga caacacagaa cgatgctgca gcagacaaga cgcgcggcgc
      481 ggcttcggtc ccaaaccgaa agcaaaaatt cagacggagg cgggaactgt tttaggttct
      541 cgtctcctac cagaaccaca tatccctcct ctaagggggg tgcaccaaag agtccaaaac
      601 gatcgggatt tttggactca ggtcgggcca caaaaacggc ccccgaagtc cctgggacgt
      661 ctcccagggt tgcggccggg tgttccgaac tcgtcagttc caccacgggt ccgccagata
      721 cagagctagt tagctaacta gtaccgacgc aggcgcataa aatcagtcat agacactaga
      781 caatcggaca gacacagata agttgctggc cagcttacct cccggtggtg ggtcggtggt
      841 ccctgggcag gggtctcccg atcccggacg agcccccaaa tgaaagaccc ccgctgacgg
      901 gtagtcaatc actcagagga gaccctccca aggaacagcg agaccacaag tcggatgcaa
      961 ctgcaagagg gtttattgga tacacgggta cccgggcgac tcagtcaatc ggaggactgg
     1021 cgcgccgagt gaggggttgt gggctctttt attgagctcg gggagcagaa gcgcgcgaac
     1081 agaagcgaga agcgaactga ttggttagtt caaataaggc acagggtcat ttcaggtcct
     1141 tggggcaccc tggaaacatc tgatggttct ctagaaactg ctgagggctg gaccgcatct
     1201 ggggaccatc tgttcttggc cctgagccgg ggcaggaact gcttaccaca gatatcctgt
     1261 ttggcccata ttcagctgtt ccatctgttc ttggccctga gccggggcag gaactgctta
     1321 ccacagatat cctgtttggc ccatattcag ctgttccatc tgttcctgac cttgatctga
     1381 acttctctat tctcagttat gtatttttcc atgccttgca aaatggcgtt acttaagcta
     1441 gcttgccaaa cctacaggtg gggtctttca ttcccccctt tttctggaga ctaaataaaa
     1501 tcttttattt tatctatggc tcgtactcta taggcttcag ctggtgatat tgttgagtca
     1561 aaactagagc ctggaccact gatatcctgt ctttaacaaa ttggactaat cgatgataag
     1621 ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
     1681 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     1741 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     1801 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     1861 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     1921 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     1981 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     2041 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
     2101 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     2161 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     2221 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     2281 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     2341 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa
     2401 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     2461 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     2521 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     2581 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     2641 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     2701 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     2761 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     2821 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     2881 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2941 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3001 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     3061 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3121 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3181 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3241 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3301 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3361 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3421 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3481 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3541 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     3601 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     3661 attttctcct tacgcatctg tgcggtattt cacaccgcat atgaaagacc cccgctgacg
     3721 ggtagtcaat cactcagagg agaccctccc aaggaacagc gagaccacaa gtcggatgca
     3781 actgcaagag ggtttattgg atacacgggt acccgggcga ctcagtcaat cggaggactg
     3841 gcgcgccgag tgaggggttg tgggctcttt tattgagctc ggggagcaga agcgcgcgaa
     3901 cagaagcgag aagcgaactg attggttagt tcaaataagg cacagggtca tttcaggtcc
     3961 ttggggcacc ctggaaacat ctgatggttc tctagaaact gctgagggct ggaccgcatc
     4021 tggggaccat ctgttcttgg ccctgagccg gggcaggaac tgcttaccac agatatcctg
     4081 tttggcccat attcagctgt tccatctgtt cttggccctg agccggggca ggaactgctt
     4141 accacagata tcctgtttgg cccatattca gctgttccat ctgttcctga ccttgatctg
     4201 aacttctcta ttctcagtta tgtatttttc catgccttgc aaaatggcgt tacttaagct
     4261 tggattgaga aagagtgagt gatggtgtgt ggaggagtat aaagcctcga cttgccaaac
     4321 ctacaggtgg ggtctttcat tccccccttt ttctggagac taaataaaat cttttatttt
     4381 atctatggct cgtactctat aggcttcagc tggtgatatt gttgagtcaa aactagagcc
     4441 tggaccactg atatcctgtc tttaacaaat tggactaatc gattaagaat gcagggtccg
     4501 aagagcaaaa tcattaggag tacaatgagg ggtcccataa tggtagatat caaggtggta
     4561 aaccaagggg atctgttaaa cagtccctca aaccatcctt gagttgactc aaacagtttc
     4621 tgtctctgat taagcctctc tctcaatttg gccatgctgt ctctcactag tcctgtgtgg
     4681 tccgcataga agcaacattc ttcttttaga gcagcacaca gccctccttc ttttagaaat
     4741 aacaagtcta ggccccttcg attctgtagg acaacttcag acagggaagt gagagacttt
     4801 tctaggttag agattgattt ttcaacctcc ctgagatcat cctgtactgc ggcttggagc
     4861 tgctggaatt gctgagtggc cattagagca gtagtccctg ttcctattcc agcggcaatt
     4921 ccccccatgc tcgaggcatg cccatgggcg gccgcgtcga ccaattgaag cttttacttg
     4981 tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg
     5041 tgatcgcgct tctcgttggg gtctttgctc agggcggact gggtgctcag gtagtggttg
     5101 tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc
     5161 tgcacgctgc cgtcctcgat gttgtggcgg gtcttgaagt tcaccttgat gccgttcttc
     5221 tgcttgtcgg cggtgatata gaccttgtgg ctgttgtagt tgtactccag cttgtgcccc
     5281 aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg
     5341 tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg
     5401 gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg
     5461 tggtcggggt agcgggcgaa gcactgcacg ccgtaggtca aggtggtcac gagggtgggc
     5521 cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg
     5581 gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc
     5641 tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac catggatcca
     5701 tgcatattct cagacaaata cagaaacaca gtcagacaga gacaacacag aacgatgctg
     5761 cagcagacaa gacgcgcggc gcggcttcgg tcccaaaccg aaagcaaaaa ttcagacgga
     5821 ggcgggaact gttttaggtt ctcgtctcct accagaacca catatccctc ctctaagggg
     5881 ggtgcaccaa agagtccaaa acgatcggga tttttggact caggtcgggc cacaaaaacg
     5941 gcccccgaag tccctgggac gtctcccagg gttgcggccg ggggttccga actcgtcagt
     6001 tccaccacgg gtccgccaga tacagagcta gttagctaac ta
//
