LOCUS       sequence-207190-        5255 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of MMLV proviral DNA-mCherry. Use together with 
            pCMV-intron and VSV-G to package MMLV reporter virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Schlesinger S, Goff SP
  TITLE     Silencing of proviruses in embryonic cells: efficiency, stability 
            and chromatin modifications.
  JOURNAL   EMBO Rep. 2013 Jan;14(1):73-9. doi: 10.1038/embor.2012.182. Epub 
            2012 Nov 16.
  PUBMED    23154467
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/embor.2012.182"; journalName: "EMBO Rep"; date: "2013-01"; 
            volume: "14"; issue: "1"; pages: "73-9"
COMMENT     Sequence Label: PNCA-mCherry
FEATURES             Location/Qualifiers
     source          1..5255
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             1..594
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    657..1014
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1082..1792
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1227..1245)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1495..1514
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     LTR             2557..3026
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3093..3110)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3264..3852)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3344..3363)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4023..4814,4815..4883))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4646..4665
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4884..4988)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5056..5074
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa cagatggaac
      121 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
      181 aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
      241 ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct
      301 agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt
      361 tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca
      421 ataaaagagc ccacaacccc tcactcggcg cgccagtcct ccgattgact gagtcgcccg
      481 ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc
      541 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg
      601 ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc
      661 tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgactga ttttatgcgc
      721 ctgcgtcggt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
      781 gagttcggaa cacccggccg caaccctggg agacgtccca gggacttcgg gggccgtttt
      841 tgtggcccga cctgagtcca aaaatcccga tcgttttgga ctctttggtg cacccccctt
      901 agaggaggga tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc
      961 tgaatttttg ctttcggttt gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat
     1021 cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg tctgagaata tgcatggatc
     1081 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     1141 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     1201 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     1261 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     1321 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     1381 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     1441 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     1501 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     1561 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     1621 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     1681 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     1741 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt aactcgagca
     1801 tggggggaat tgccgctgga ataggaacag ggactactgc tctaatggcc actcagcaat
     1861 tccagcagct ccaagccgca gtacaggatg atctcaggga ggttgaaaaa tcaatctcta
     1921 acctagaaaa gtctctcact tccctgtctg aagttgtcct acagaatcga aggggcctag
     1981 acttgttatt tctaaaagaa ggagggctgt gtgctgctct aaaagaagaa tgttgcttct
     2041 atgcggacca cacaggacta gtgagagaca gcatggccaa attgagagag aggcttaatc
     2101 agagacagaa actgtttgag tcaactcaag gatggtttga gggactgttt aacagatccc
     2161 cttggtttac caccttgata tctaccatta tgggacccct cattgtactc ctaatgattt
     2221 tgctcttcgg accctgcatt cttaatcgat tagtccaatt tgttaaagac aggatatcag
     2281 tggtccaggc tctagttttg actcaacaat atcaccagct gaagcctata gagtacgagc
     2341 catagataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca
     2401 cctgtaggtt tggcaagtcg aggctttata ctcctccaca caccatcact cactctttct
     2461 caatccaagc ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag
     2521 agaagttcag atcaaggtca ggaacagatg gaacagctga atatgggcca aacaggatat
     2581 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggaaca gctgaatatg
     2641 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
     2701 gtccccagat gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt
     2761 gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc
     2821 gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc
     2881 ggcgcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc
     2941 ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt
     3001 gactacccgt cagcgggggt ctttcatatg cggtgtgaaa taccgcacag atgcgtaagg
     3061 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     3121 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     3181 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     3241 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     3301 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3361 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     3421 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     3481 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3541 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3601 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3661 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     3721 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3781 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3841 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3901 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     3961 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     4021 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     4081 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     4141 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     4201 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     4261 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     4321 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     4381 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     4441 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     4501 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     4561 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     4621 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg
     4681 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     4741 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     4801 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     4861 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     4921 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     4981 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     5041 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attctcatgt
     5101 ttgacagctt atcatcgatt agtccaattt gttaaagaca ggatatcagt ggtccaggct
     5161 ctagttttga ctcaacaata tcaccagctg aagcctatag agtacgagcc atagataaaa
     5221 taaaagattt tatttagtct ccagaaaaag ggggg
//
