LOCUS       sequence-207200-        9446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human codon optimized inactive FnCpf1(dead1) in mammalian 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9446)
  AUTHORS   Toth E, Czene BC, Kulcsar PI, Krausz SL, Talas A, Nyeste A, Varga E,
            Huszar K, Weinhardt N, Ligeti Z, Borsy AE, Fodor E, Welker E
  TITLE     Mb- and FnCpf1 nucleases are active in mammalian cells: activities 
            and PAM preferences of four wild-type Cpf1 nucleases and of their 
            altered PAM specificity variants.
  JOURNAL   Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 
            10.1093/nar/gky815.
  PUBMED    30239882
REFERENCE   2  (bases 1 to 9446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815."
COMMENT     Sequence Label: pTE4999
FEATURES             Location/Qualifiers
     source          1..9446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        80..459
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        460..663
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     614..634
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4674..4721
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4728..4808
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(4882..4899)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4888..5112
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5158..5586
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5245..5264)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5455..5476
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5595..5615)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5600..5929
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5780..5915
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5842..5861
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5996..6790
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6050..6069)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6660..6679
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6964..7085
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7001..7020)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7055..7074
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7134..7150)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7134..7150)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7147..7169)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7158..7174
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7182..7188,7189..7206,7207..7212))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7227..7248
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7365..7382)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7536..8124)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7616..7635)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8295..9086,9087..9155))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8918..8937
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9156..9260)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9335..9354)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
       61 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
      121 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
      181 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
      241 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
      301 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
      361 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
      421 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
      481 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
      541 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
      601 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
      661 gctctctggc taactagaga acccactgct tactggctta tcgaaattga tacgactcac
      721 tatagggaga cccaagctgg ctagcgttta aacttaagct tggtaccgcc accatgagca
      781 tctaccagga gttcgtcaac aagtattcac tgagtaagac actgcggttc gagctgatcc
      841 cacagggcaa gacactggag aacatcaagg cccgaggcct gattctggac gatgagaagc
      901 gggcaaaaga ctataagaaa gccaagcaga tcattgataa ataccaccag ttctttatcg
      961 aggaaattct gagctccgtg tgcatcagtg aggatctgct gcagaattac tcagacgtgt
     1021 acttcaagct gaagaagagc gacgatgaca acctgcagaa ggacttcaag tccgccaagg
     1081 acaccatcaa gaaacagatt agcgagtaca tcaaggactc cgaaaagttt aaaaatctgt
     1141 tcaaccagaa tctgatcgat gctaagaaag gccaggagtc cgacctgatc ctgtggctga
     1201 aacagtctaa ggacaatggg attgaactgt tcaaggctaa ctccgatatc actgatattg
     1261 acgaggcact ggaaatcatc aagagcttca agggatggac cacatacttt aaaggcttcc
     1321 acgagaaccg caagaacgtg tactccagca acgacattcc tacctccatc atctaccgaa
     1381 tcgtcgatga caatctgcca aagttcctgg agaacaaggc caaatatgaa tctctgaagg
     1441 acaaagctcc cgaggcaatt aattacgaac agatcaagaa agatctggct gaggaactga
     1501 cattcgatat cgactataag actagcgagg tgaaccagag ggtcttttcc ctggacgagg
     1561 tgtttgaaat cgccaatttc aacaattacc tgaaccagtc cggcattact aaattcaata
     1621 ccatcattgg cgggaagttt gtgaacgggg agaataccaa gcgcaaggga attaacgaat
     1681 acatcaatct gtatagccag cagatcaacg acaaaactct gaagaaatac aagatgtctg
     1741 tgctgttcaa acagatcctg agtgataccg agtccaagtc ttttgtcatt gataaactgg
     1801 aagatgactc agacgtggtc actaccatgc agagctttta tgagcagatc gccgctttca
     1861 agacagtgga ggaaaaatct attaaggaaa ctctgagtct gctgttcgat gacctgaaag
     1921 cccagaagct ggacctgagt aagatctact tcaaaaacga taagagtctg acagacctgt
     1981 cacagcaggt gtttgatgac tattccgtga ttgggaccgc cgtcctggag tacattacac
     2041 agcagatcgc tccaaagaac ctggataatc cctctaagaa agagcaggaa ctgatcgcta
     2101 agaaaaccga gaaggcaaaa tatctgagtc tggaaacaat taagctggca ctggaggagt
     2161 tcaacaagca cagggatatt gacaaacagt gccgctttga ggaaatcctg gccaacttcg
     2221 cagccatccc catgattttt gatgagatcg cccagaacaa agacaatctg gctcagatca
     2281 gtattaagta ccagaaccag ggcaagaaag acctgctgca ggcttcagca gaagatgacg
     2341 tgaaagccat caaggatctg ctggaccaga ccaacaatct gctgcacaag ctgaaaatct
     2401 tccatattag tcagtcagag gataaggcta atatcctgga taaagacgaa cacttctacc
     2461 tggtgttcga ggaatgttac ttcgagctgg caaacattgt ccccctgtat aacaagatta
     2521 ggaactacat cacacagaag ccttactctg acgagaagtt taaactgaac ttcgaaaata
     2581 gtaccctggc caacgggtgg gataagaaca aggagcctga caacacagct atcctgttca
     2641 tcaaggatga caagtactat ctgggagtga tgaataagaa aaacaataag atcttcgatg
     2701 acaaagccat taaggagaac aaaggggaag gatacaagaa aatcgtgtat aagctgctgc
     2761 ccggcgcaaa taagatgctg cctaaggtgt tcttcagcgc caagagtatc aaattctaca
     2821 acccatccga ggacatcctg cggattagaa atcactcaac acatactaag aacgggagcc
     2881 cccagaaggg atatgagaaa tttgagttca acatcgagga ttgcaggaag tttattgact
     2941 tctacaagca gagcatctcc aaacaccctg aatggaagga ttttggcttc cggttttccg
     3001 acacacagag atataactct atcgacgagt tctaccgcga ggtggaaaat caggggtata
     3061 agctgacttt tgagaacatt tctgaaagtt acatcgacag cgtggtcaat cagggaaagc
     3121 tgtacctgtt ccagatctat aacaaagatt tttcagcata cagcaagggc agaccaaacc
     3181 tgcatacact gtactggaag gccctgttcg atgagaggaa tctgcaggac gtggtctata
     3241 aactgaacgg agaggccgaa ctgttttacc ggaagcagtc tattcctaag aaaatcactc
     3301 acccagctaa ggaggccatc gctaacaaga acaaggacaa tcctaagaaa gagagcgtgt
     3361 tcgaatacga tctgattaag gacaagcggt tcaccgaaga taagttcttt ttccattgtc
     3421 caatcaccat taacttcaag tcaagcggcg ctaacaagtt caacgacgag atcaatctgc
     3481 tgctgaagga aaaagcaaac gatgtgcaca tcctgagcat tgctcgagga gagcggcatc
     3541 tggcctacta taccctggtg gatggcaaag ggaatatcat taagcaggat acattcaaca
     3601 tcattggcaa tgaccggatg aaaaccaact accacgataa actggctgca atcgagaagg
     3661 atagagactc agctaggaag gactggaaga aaatcaacaa cattaaggag atgaaggaag
     3721 gctatctgag ccaggtggtc catgagattg caaagctggt catcgaatac aatgccattg
     3781 tggtgttcga ggatctgaac ttcggcttta agagggggcg ctttaaggtg gaaaaacagg
     3841 tctatcagaa gctggagaaa atgctgatcg aaaagctgaa ttacctggtg tttaaagata
     3901 acgagttcga caagaccgga ggcgtcctga gagcctacca gctgacagct ccctttgaaa
     3961 ctttcaagaa aatgggaaaa cagacaggca tcatctacta tgtgccagcc ggattcactt
     4021 ccaagatctg ccccgtgacc ggctttgtca accagctgta ccctaaatat gagtcagtga
     4081 gcaagtccca ggaatttttc agcaagttcg ataagatctg ttataatctg gacaaggggt
     4141 acttcgagtt ttccttcgat tacaagaact tcggcgacaa ggccgctaag gggaaatgga
     4201 ccattgcctc cttcggatct cgcctgatca actttcgaaa ttccgataaa aaccacaatt
     4261 gggacactag ggaggtgtac ccaaccaagg agctggaaaa gctgctgaaa gactactcta
     4321 tcgagtatgg acatggcgaa tgcatcaagg cagccatctg tggcgagagt gataagaaat
     4381 ttttcgccaa gctgacctca gtgctgaata caatcctgca gatgcggaac tcaaagaccg
     4441 ggacagaact ggactatctg attagccccg tggctgatgt caacggaaac ttcttcgaca
     4501 gcagacaggc acccaaaaat atgcctcagg atgcagacgc caacggggcc taccacatcg
     4561 ggctgaaggg actgatgctg ctgggccgga tcaagaacaa tcaggagggg aagaagctga
     4621 acctggtcat taagaacgag gaatacttcg agtttgtcca gaatagaaat aacaaaaggc
     4681 cggcggccac gaaaaaggcc ggccaggcaa aaaagaaaaa gggatcctac ccatacgatg
     4741 ttccagatta cgcttatccc tacgacgtgc ctgattatgc atacccatat gatgtccccg
     4801 actatgccta agaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc
     4861 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     4921 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     4981 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     5041 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     5101 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     5161 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     5221 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     5281 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     5341 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     5401 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     5461 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     5521 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     5581 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     5641 agacagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     5701 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     5761 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     5821 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     5881 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     5941 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
     6001 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     6061 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     6121 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     6181 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     6241 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     6301 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     6361 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     6421 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     6481 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     6541 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     6601 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     6661 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     6721 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     6781 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     6841 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     6901 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     6961 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     7021 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     7081 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     7141 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     7201 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     7261 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     7321 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     7381 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7441 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7501 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7561 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7621 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7681 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7741 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7801 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7861 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7921 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7981 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     8041 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     8101 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     8161 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     8221 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8281 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8341 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8401 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8461 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8521 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8581 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     8641 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8701 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     8761 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     8821 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8881 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8941 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     9001 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     9061 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     9121 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9181 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     9241 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     9301 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     9361 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
     9421 tttaagctac aacaaggcaa ggcttg
//
