LOCUS       sequence-207225-        4043 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Negative regulator of cell cycle progression by G1/S transition 
            inhibition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Razy-Krajka F, Gravez B, Kaplan N, Racioppi C, Wang W, Christiaen L
  TITLE     An FGF-driven feed-forward circuit patterns the cardiopharyngeal 
            mesoderm in space and time.
  JOURNAL   Elife. 2018 Feb 6;7. pii: 29656. doi: 10.7554/eLife.29656.
  PUBMED    29431097
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-02-6"; volume: "7. pii: 29656. doi"; pages: " 
            10.7554/eLife.29656"
COMMENT     Sequence Label: FoxFTVC+bpFOG_Cdkn1b.b
FEATURES             Location/Qualifiers
     source          1..4043
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1761..1895
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1798..1817)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1852..1871
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1931..1950)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1932..1950)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2037..2054)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2208..2796)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2288..2307)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2967..3758,3759..3827))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3590..3609
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3828..3932)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4000..4018
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcgttgtatc tttcgttaag tgctggcgtc gagcagggct aacctcattt cgatttgacg
       61 attcgggtaa aaccgagcga tggacaggac ctggaaatgg gaaaggctta ctagaatcag
      121 ggtcgaacga gataagaaga tcaggattcg caaattagca cttggctttt ttacgtagaa
      181 acataggatg tccgctgagc cccttatata tacgtagtac gaatcaccgg cctggcgttg
      241 tataagcgag accagcgtgg acgaaaaggt aattgtttcc gaatggatcg cgttctctag
      301 aggatccggc aaagcttcgt gtattgtacc ggcccattgt caatcatgca aacttgatat
      361 tatattgaca agagaagaag gcagtttaaa ttaaaactct aaagtagaga gacattaatc
      421 tcagctgaca aggcaggtgg tcacagtaag ttcatttaaa tagttggcca acaatagcct
      481 ttccaagaaa gtatttttgt tccaggtcta tacaaaaata acacacaagc ggccgcaacc
      541 atggttcccc caccttcgta caatgcccac gaaacatcag cattcgctgt ttttgagaag
      601 ccagtgctag tgaagccccc aagtcctgtt tgtcgccaac tatttggtct ttcagatcaa
      661 ttagagacga gcagacgact taaagaggtt ttggattctg aagctaaaag atttcagttc
      721 gcctggaact tcgacgtccg taaagttcca gctgcgggaa atcagtcgcc ggatcccggg
      781 gcagctgcac cgggcaaacc gaagcgtttt gagtggaagt tacaacaaag aagtgaagtg
      841 ccacacttct acactcgacc ctttgcaacc cacaggttgg caatgacgtc acctcgtctc
      901 gaccgttccc cattgaaaaa gaaccgcacc cagaaaagcc accacgatag acacctaaca
      961 aacacttcct ctccgacgcc ccttagtcaa cgagatgaaa acgtctcccg atcgatcata
     1021 aatccatcta aacgactttg catccccctt tttacgagcc cacctaagct aaccaccgaa
     1081 gcgaagccag acttgaccag ggttcctgca atgagaattc ccccgattac gccaagcgcc
     1141 gactccaaca agcccagcat ggttagtgac gacatggaaa aggcaaccgc atcggagcca
     1201 gtcgggtcca tggtcgcttc caactcattg aagcggcctg cctctcgaaa gttgttcaac
     1261 ctgagcactc caagtccaaa gcgatcgcga actagcagcc gacaaccgaa aataacagac
     1321 gtgatgccac aacgaaaact ccgttcaacg tcgaacaaca aaagcgaagg caataaaact
     1381 gtgaacccca taaactctgc ctctttgcaa gcagccaaag caagtaattg atacgaaaat
     1441 gaattccagc tgagcgccgg tcgctaccat taccagttgg tctggtgtca aaaataataa
     1501 taaccgggca ggccatgtct gcccgtattt cgcgtaagga aatccattat gtactattta
     1561 aaaaacacaa acttttggat gttcggttta ttctttttct tttacttttt tatcatggga
     1621 gcctacttcc cgtttttccc gatttggcta catgacatca accatatcag caaaagtgat
     1681 acgggtatta tttttgccgc tatttctctg ttctcgctat tattccaacc gctgtttggt
     1741 ctgctttctg acaaactcgg aacttgttta ttgcagctta taatggttac aaataaagca
     1801 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     1861 ccaaactcat caatgtatct tatcatgtct ggatcgacaa agtcaaagcg gccatcagat
     1921 ctgccggtct ccctatagtg agtcgtatta atttcgataa gccaggttaa cctgcattaa
     1981 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     2041 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     2101 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     2161 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     2221 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2281 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     2341 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2401 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     2461 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     2521 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     2581 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     2641 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     2701 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     2761 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     2821 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     2881 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     2941 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     3001 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     3061 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     3121 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     3181 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     3241 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     3301 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3361 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     3421 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     3481 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     3541 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     3601 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     3661 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     3721 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     3781 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     3841 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3901 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     3961 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     4021 ttacgtatta attaaggcgc gcc
//
