LOCUS       sequence-207228-        9428 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing FAM149a, puromycin resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9428)
  AUTHORS   Aries IM, Bodaar K, Karim SA, Chonghaile TN, Hinze L, Burns MA, 
            Pfirrmann M, Degar J, Landrigan JT, Balbach S, Peirs S, Menten B, 
            Isenhart R, Stevenson KE, Neuberg DS, Devidas M, Loh ML, Hunger SP, 
            Teachey DT, Rabin KR, Winter SS, Dunsmore KP, Wood BL, Silverman LB,
            Sallan SE, Van Vlierberghe P, Orkin SH, Knoechel B, Letai AG, 
            Gutierrez A
  TITLE     PRC2 loss induces chemoresistance by repressing apoptosis in T cell 
            acute lymphoblastic leukemia.
  JOURNAL   J Exp Med. 2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570.
  PUBMED    30404791
REFERENCE   2  (bases 1 to 9428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Med. 
            2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570."
COMMENT     Sequence Label: pLenti CMV Puro DEST (w118-1) FAM149A
FEATURES             Location/Qualifiers
     source          1..9428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..590
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     491..510
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     744..761
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    878..899
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(914..919,920..937,938..944)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    952..968
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     957..979
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     976..992
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     976..992
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1011..1031
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1013..1031
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1059..1285
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1286..1466
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1513..1638
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2131..2364
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2549..2593
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2860..2976
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2999..3302
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3303..3506
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3457..3477
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3503..3527
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3611..3635
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5091..5115)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5146..5734
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5199..5219)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5617..5628)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5748..6247
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5783..5806)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6180..6199
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6268..6867
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6268..6287)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6764..6784
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7005..7238
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7310..7431
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7347..7366)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7401..7420
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7471..7606
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7626..7645)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7627..7645)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7655..7672)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7655..7671)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7664..7686)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7813..8268
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7900..7919)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8110..8131
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8294..8398
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8399..8467,8468..9259)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8617..8636)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
       61 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
      121 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
      181 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
      241 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
      301 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
      361 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      421 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      481 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      541 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      601 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      661 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      721 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
      781 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
      841 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
      901 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
      961 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     1021 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
     1081 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     1141 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     1201 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
     1261 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
     1321 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     1381 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     1441 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     1501 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     1561 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     1621 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     1681 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     1741 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     1801 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     1861 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     1921 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     1981 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     2041 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     2101 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     2161 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     2221 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     2281 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     2341 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     2401 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     2461 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     2521 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     2581 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     2641 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     2701 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     2761 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     2821 cattcgatta gtgaacggat ctcgacggta tcggttaact tttaaaagaa aaggggggat
     2881 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2941 agaattacaa aaacaaatta caaaattcaa aattttatcg ataagcttgg gagttccgcg
     3001 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     3061 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     3121 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     3181 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     3241 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     3301 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     3361 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     3421 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     3481 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc
     3541 atccacgctg ttttgacctc catagaagac accgactcta gtccagtgtg gtggaattct
     3601 gcagatatca acaagtttgt acaaaaaagc aggcttcacc atgttatttg aagggaaagt
     3661 gaaccctcag acccagagtc tgctggccga atgcggggag tggacaagaa gatccctcca
     3721 tttgagagta ttaggaagac agctgatcct gcccactgac aaaggcgtcc agcatttcca
     3781 gggcagcact cctgcctccg cagtccacag acccccgctc agtgcctgcg gacacagcag
     3841 caacatcaga gagttgtgca tttctggctc tcaaatagtc ccagcagcac tctcagcctc
     3901 tgccctgcca ggccctgatg acacaggggt tgctgaccta acggcacgtt catccctgga
     3961 agaagaggtt taccatgtgg atggaaagat tgaggagtat ttcgcttttg acagaaaaga
     4021 ggatgatgat gaatgtcttg aacaaaaacc agctcagccc ggtaggaaat ggcgcaaact
     4081 cggacttcct cctgtttccc cgcgtgactg tgtcaaagat gccgtggcag cagaagtgtt
     4141 tgatcacgtc tggacaaata tggtagaact tttggaagag ctgattagaa aacactggga
     4201 aactacactc acagaaggga aaaaacagag agaaacattg aaagtggctg gaaacagatt
     4261 tccgcacgtc ctcgttccac acgctcacgc cgatggagcc agtggccccc cgtccggaca
     4321 cgccgaggct cacggcatct ccctggcttc tcgtctgaac ccgccccaga ttcatcactt
     4381 ctccagcagt ttttatagtg acatgaatgg tgtcatgaca attcaagcaa aaccgcttca
     4441 gcggagacct gcctattttg ctgacagaac gcagaatgag aaggaggaca aagcatcggg
     4501 tggaggggca ggtgctctct cctccgcacc gcacagactg ggacgggcct cagacactca
     4561 tggattatca ccttctgcaa agaaaacacc agtgccctgg aggctgcctt ctcttgcttc
     4621 agattcacag agactaaaaa ctcccaacat ctatagtgac gaagttcttc ggggaacaaa
     4681 actgccgact ggcgtggacc acatggcttc cccactggtt caaacgtcac ggagcaggtt
     4741 ccccccgcta gtcacggaga ccagggggca gaatacagca gttcctggat gccgccttgt
     4801 ttcttacaga ggaaggcatc tacaaaaccg tgtgttgagt gccatgcctg acggtacaga
     4861 acgatcgcgt cttcgagaaa gaacagccac cctggaacgg ttgtcaaggc ccagcacaac
     4921 ccacacgttc cggcagtcgg atacgcctcg aaaaagttca ttgacacaaa tggaatttgc
     4981 tgctcacaca tggacaggtc aaagtatttt gacaggttca caatatgtgc ctaaatcttt
     5041 tcagaggaca actttgactt tcaagaggag attccaagtg acatcttgaa acccagcttt
     5101 cttgtacaaa gtggttgata tccagcacag tggcggccgc tcgacaatca acctctggat
     5161 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     5221 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     5281 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     5341 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     5401 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     5461 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     5521 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     5581 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     5641 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     5701 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttctaccggg taggggaggc
     5761 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     5821 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
     5881 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     5941 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     6001 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     6061 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     6121 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
     6181 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
     6241 cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc
     6301 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     6361 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     6421 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     6481 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     6541 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     6601 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     6661 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     6721 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     6781 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     6841 tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa
     6901 aggagcgcac gaccccatgc atctcgaggg cccggtacct ttaagaccaa tgacttacaa
     6961 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctagctca
     7021 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     7081 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     7141 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     7201 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     7261 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     7321 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     7381 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     7441 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     7501 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     7561 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     7621 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     7681 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     7741 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     7801 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     7861 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     7921 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     7981 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     8041 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     8101 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     8161 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     8221 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     8281 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     8341 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     8401 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     8461 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     8521 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     8581 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     8641 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     8701 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     8761 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     8821 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     8881 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     8941 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     9001 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     9061 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     9121 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     9181 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     9241 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     9301 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     9361 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     9421 aggatctt
//
