LOCUS       sequence-207231-         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing FAM149a, puromycin resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Aries IM, Bodaar K, Karim SA, Chonghaile TN, Hinze L, Burns MA, 
            Pfirrmann M, Degar J, Landrigan JT, Balbach S, Peirs S, Menten B, 
            Isenhart R, Stevenson KE, Neuberg DS, Devidas M, Loh ML, Hunger SP, 
            Teachey DT, Rabin KR, Winter SS, Dunsmore KP, Wood BL, Silverman LB,
            Sallan SE, Van Vlierberghe P, Orkin SH, Knoechel B, Letai AG, 
            Gutierrez A
  TITLE     PRC2 loss induces chemoresistance by repressing apoptosis in T cell 
            acute lymphoblastic leukemia.
  JOURNAL   J Exp Med. 2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570.
  PUBMED    30404791
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Med. 
            2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570."
COMMENT     Sequence Label: pLenti CMV Puro DEST (w118-1) FAM149A
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(866..890)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cagagagaaa cattgaaagt ggctggaaac agatttccgc acgtcctcgt tccacacgct
       61 cacgccgatg gagccagtgg ccccccgtcc ggacacgccg aggctcacgg catctccctg
      121 gcttctcgtc tgaacccgcc ccagattcat cacttctcca gcagttttta tagtgacatg
      181 aatggtgtca tgacaattca agcaaaaccg cttcagcgga gacctgccta ttttgctgac
      241 agaacgcaga atgagaagga ggacaaagca tcgggtggag gggcaggtgc tctctcctcc
      301 gcaccgcaca gactgggacg ggcntcagac actcatggat tatcaccttc tgcaaagaaa
      361 acaccagtgc cctggaggct gccttctctt gcttcagatt cacagagact aaaaactccc
      421 aacatctata gtgacgaagt tcttcgggga acaaaactgc cgactggcgt ggaccacatg
      481 gcttccccac tggttcaaac gtcacggagc aggttccccc cgctagtcac ggagaccagg
      541 gggcagaata cagcagttcc tggatgccgc cttgtttctt acagaggaag gcatctacaa
      601 aaccgtgtgt tgagtgccat gcctgacggt acagaacgat cgcgtcttcg agaaagaaca
      661 gccaccctgg aacggttgtc aaggcccagc acaacccaca cgttccggca gtcggatacg
      721 cctcgaaaaa gttcattgac acaaatggaa tttgctgctc acacatggac aggtcaaagt
      781 attttgacag gttcacaata tgtgcctaaa tcttttcaga ggacaacttt gactttcaag
      841 aggagattcc aagtgacatc ttgaaaccca gctttcttgt acaaagtggt tgatatccag
      901 cacagtggcg gccgctcgac a
//
