LOCUS       sequence-207235-        9486 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Lb crRNA and human codon optimized LbCpf1(RR mutant) in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9486)
  AUTHORS   Toth E, Czene BC, Kulcsar PI, Krausz SL, Talas A, Nyeste A, Varga E,
            Huszar K, Weinhardt N, Ligeti Z, Borsy AE, Fodor E, Welker E
  TITLE     Mb- and FnCpf1 nucleases are active in mammalian cells: activities 
            and PAM preferences of four wild-type Cpf1 nucleases and of their 
            altered PAM specificity variants.
  JOURNAL   Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 
            10.1093/nar/gky815.
  PUBMED    30239882
REFERENCE   2  (bases 1 to 9486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815."
COMMENT     Sequence Label: pTE3329
FEATURES             Location/Qualifiers
     source          1..9486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        complement(214..462)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(272..291)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(442..462)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     enhancer        525..828
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        829..1032
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     983..1003
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1077..1096
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1077..1095
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1131..1140
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1143..1163
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1188..4868
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAED
                     YKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEI
                     AKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFSE
                     EAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGE
                     FFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLS
                     DRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNG
                     PAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL
                     QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDL
                     LDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSK
                     DKFKLYFQNPQFMRGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGN
                     YEKINYKLLPGPNKMLPRVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLI
                     DFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVE
                     EGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE
                     ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKIN
                     TEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDY
                     HSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGF
                     KNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQN
                     GFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDY
                     KNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQ
                     GDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNY
                     EAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSV
                     KH"
     CDS             4869..4916
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4923..5003
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(5077..5094)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5083..5307
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5353..5781
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5440..5459)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5650..5671
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5790..5810)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5795..6124
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5975..6110
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6037..6056
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6191..6985
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6245..6264)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6855..6874
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7159..7280
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7196..7215)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7250..7269
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7329..7345)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7329..7345)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7342..7364)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7353..7369
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7377..7383,7384..7401,7402..7407))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7422..7443
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7560..7577)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7731..8319)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7811..7830)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8490..9281,9282..9350))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9113..9132
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9351..9455)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattaaaaa aggagacggg
      181 atcccgtctc catctacact tagtagaaat tacggtgttt cgtcctttcc acaagatata
      241 taaagccaag aaatcgaaat actttcaagt tacggtaagc atatgatagt ccattttaaa
      301 acataatttt aaaactgcaa actacccaag aaattattac tttctacgtc acgtattttg
      361 tactaatatc tttgtgttta cagtcaaatt aattccaatt atctctctaa cagccttgta
      421 tcgtatatgc aaatatgaag gaatcatggg aaataggccc tcactagtta ttaatagtaa
      481 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      541 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      601 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      661 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
      721 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
      781 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      841 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      901 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      961 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     1021 ataagcagag ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat
     1081 acgactcact atagggagac ccaagctggc tagcgtttaa acttaagctt gccaccatgg
     1141 ccccaaagaa gaagcggaag gtcggtatcc acggagtccc agcagccagc aagctggaga
     1201 agtttacaaa ctgctactcc ctgtctaaga ccctgaggtt caaggccatc cctgtgggca
     1261 agacccagga gaacatcgac aataagcggc tgctggtgga ggacgagaag agagccgagg
     1321 attataaggg cgtgaagaag ctgctggatc gctactatct gtcttttatc aacgacgtgc
     1381 tgcacagcat caagctgaag aatctgaaca attacatcag cctgttccgg aagaaaacca
     1441 gaaccgagaa ggagaataag gagctggaga acctggagat caatctgcgg aaggagatcg
     1501 ccaaggcctt caagggcaac gagggctaca agtccctgtt taagaaggat atcatcgaga
     1561 caatcctgcc agagttcctg gacgataagg acgagatcgc cctggtgaac agcttcaatg
     1621 gctttaccac agccttcacc ggcttctttg ataacagaga gaatatgttt tccgaggagg
     1681 ccaagagcac atccatcgcc ttcaggtgta tcaacgagaa tctgacccgc tacatctcta
     1741 atatggacat cttcgagaag gtggacgcca tctttgataa gcacgaggtg caggagatca
     1801 aggagaagat cctgaacagc gactatgatg tggaggattt ctttgagggc gagttcttta
     1861 actttgtgct gacacaggag ggcatcgacg tgtataacgc catcatcggc ggcttcgtga
     1921 ccgagagcgg cgagaagatc aagggcctga acgagtacat caacctgtat aatcagaaaa
     1981 ccaagcagaa gctgcctaag tttaagccac tgtataagca ggtgctgagc gatcgggagt
     2041 ctctgagctt ctacggcgag ggctatacat ccgatgagga ggtgctggag gtgtttagaa
     2101 acaccctgaa caagaacagc gagatcttca gctccatcaa gaagctggag aagctgttca
     2161 agaattttga cgagtactct agcgccggca tctttgtgaa gaacggcccc gccatcagca
     2221 caatctccaa ggatatcttc ggcgagtgga acgtgatccg ggacaagtgg aatgccgagt
     2281 atgacgatat ccacctgaag aagaaggccg tggtgaccga gaagtacgag gacgatcgga
     2341 gaaagtcctt caagaagatc ggctcctttt ctctggagca gctgcaggag tacgccgacg
     2401 ccgatctgtc tgtggtggag aagctgaagg agatcatcat ccagaaggtg gatgagatct
     2461 acaaggtgta tggctcctct gagaagctgt tcgacgccga ttttgtgctg gagaagagcc
     2521 tgaagaagaa cgacgccgtg gtggccatca tgaaggacct gctggattct gtgaagagct
     2581 tcgagaatta catcaaggcc ttctttggcg agggcaagga gacaaacagg gacgagtcct
     2641 tctatggcga ttttgtgctg gcctacgaca tcctgctgaa ggtggaccac atctacgatg
     2701 ccatccgcaa ttatgtgacc cagaagccct actctaagga taagttcaag ctgtattttc
     2761 agaaccctca gttcatgcgc ggctgggaca aggataagga gacagactat cgggccacca
     2821 tcctgagata cggctccaag tactatctgg ccatcatgga taagaagtac gccaagtgcc
     2881 tgcagaagat cgacaaggac gatgtgaacg gcaattacga gaagatcaac tataagctgc
     2941 tgcccggccc taataagatg ctgccaaggg tgttcttttc taagaagtgg atggcctact
     3001 ataaccccag cgaggacatc cagaagatct acaagaatgg cacattcaag aagggcgata
     3061 tgtttaacct gaatgactgt cacaagctga tcgacttctt taaggatagc atctcccggt
     3121 atccaaagtg gtccaatgcc tacgatttca acttttctga gacagagaag tataaggaca
     3181 tcgccggctt ttacagagag gtggaggagc agggctataa ggtgagcttc gagtctgcca
     3241 gcaagaagga ggtggataag ctggtggagg agggcaagct gtatatgttc cagatctata
     3301 acaaggactt ttccgataag tctcacggca cacccaatct gcacaccatg tacttcaagc
     3361 tgctgtttga cgagaacaat cacggacaga tcaggctgag cggaggagca gagctgttca
     3421 tgaggcgcgc ctccctgaag aaggaggagc tggtggtgca cccagccaac tcccctatcg
     3481 ccaacaagaa tccagataat cccaagaaaa ccacaaccct gtcctacgac gtgtataagg
     3541 ataagaggtt ttctgaggac cagtacgagc tgcacatccc aatcgccatc aataagtgcc
     3601 ccaagaacat cttcaagatc aatacagagg tgcgcgtgct gctgaagcac gacgataacc
     3661 cctatgtgat cggcatcgat aggggcgagc gcaatctgct gtatatcgtg gtggtggacg
     3721 gcaagggcaa catcgtggag cagtattccc tgaacgagat catcaacaac ttcaacggca
     3781 tcaggatcaa gacagattac cactctctgc tggacaagaa ggagaaggag aggttcgagg
     3841 cccgccagaa ctggacctcc atcgagaata tcaaggagct gaaggccggc tatatctctc
     3901 aggtggtgca caagatctgc gagctggtgg agaagtacga tgccgtgatc gccctggagg
     3961 acctgaactc tggctttaag aatagccgcg tgaaggtgga gaagcaggtg tatcagaagt
     4021 tcgagaagat gctgatcgat aagctgaact acatggtgga caagaagtct aatccttgtg
     4081 caacaggcgg cgccctgaag ggctatcaga tcaccaataa gttcgagagc tttaagtcca
     4141 tgtctaccca gaacggcttc atcttttaca tccctgcctg gctgacatcc aagatcgatc
     4201 catctaccgg ctttgtgaac ctgctgaaaa ccaagtatac cagcatcgcc gattccaaga
     4261 agttcatcag ctcctttgac aggatcatgt acgtgcccga ggaggatctg ttcgagtttg
     4321 ccctggacta taagaacttc tctcgcacag acgccgatta catcaagaag tggaagctgt
     4381 actcctacgg caaccggatc agaatcttcc ggaatcctaa gaagaacaac gtgttcgact
     4441 gggaggaggt gtgcctgacc agcgcctata aggagctgtt caacaagtac ggcatcaatt
     4501 atcagcaggg cgatatcaga gccctgctgt gcgagcagtc cgacaaggcc ttctactcta
     4561 gctttatggc cctgatgagc ctgatgctgc agatgcggaa cagcatcaca ggccgcaccg
     4621 acgtggattt tctgatcagc cctgtgaaga actccgacgg catcttctac gatagccgga
     4681 actatgaggc ccaggagaat gccatcctgc caaagaacgc cgacgccaat ggcgcctata
     4741 acatcgccag aaaggtgctg tgggccatcg gccagttcaa gaaggccgag gacgagaagc
     4801 tggataaggt gaagatcgcc atctctaaca aggagtggct ggagtacgcc cagaccagcg
     4861 tgaagcacaa aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag aaaaagggat
     4921 cctacccata cgatgttcca gattacgctt atccctacga cgtgcctgat tatgcatacc
     4981 catatgatgt ccccgactat gcctaagaat tctgcagata tccagcacag tggcggccgc
     5041 tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     5101 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     5161 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     5221 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     5281 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     5341 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     5401 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     5461 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     5521 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     5581 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     5641 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     5701 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     5761 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     5821 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     5881 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5941 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     6001 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     6061 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     6121 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg
     6181 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     6241 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     6301 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     6361 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     6421 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     6481 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     6541 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     6601 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     6661 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     6721 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     6781 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     6841 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     6901 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     6961 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     7021 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     7081 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     7141 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     7201 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7261 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     7321 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     7381 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     7441 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     7501 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     7561 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7621 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7681 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7741 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7801 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7861 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7921 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7981 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     8041 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     8101 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     8161 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     8221 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     8281 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8341 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     8401 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     8461 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8521 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8581 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8641 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8701 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8761 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8821 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8881 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8941 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     9001 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     9061 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     9121 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     9181 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     9241 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     9301 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     9361 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9421 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     9481 gacgtc
//
