LOCUS       sequence-207237-        3628 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Negative regulator of cell cycle progression by G1/S transition 
            inhibition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Razy-Krajka F, Gravez B, Kaplan N, Racioppi C, Wang W, Christiaen L
  TITLE     An FGF-driven feed-forward circuit patterns the cardiopharyngeal 
            mesoderm in space and time.
  JOURNAL   Elife. 2018 Feb 6;7. pii: 29656. doi: 10.7554/eLife.29656.
  PUBMED    29431097
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-02-6"; volume: "7. pii: 29656. doi"; pages: " 
            10.7554/eLife.29656"
COMMENT     Sequence Label: FoxFTVC+bpFOG_Cdkn1b.a
FEATURES             Location/Qualifiers
     source          1..3628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1346..1480
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1383..1402)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1437..1456
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1516..1535)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1517..1535)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1622..1639)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1793..2381)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1873..1892)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2552..3343,3344..3412))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3175..3194
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3413..3517)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3585..3603
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcgttgtatc tttcgttaag tgctggcgtc gagcagggct aacctcattt cgatttgacg
       61 attcgggtaa aaccgagcga tggacaggac ctggaaatgg gaaaggctta ctagaatcag
      121 ggtcgaacga gataagaaga tcaggattcg caaattagca cttggctttt ttacgtagaa
      181 acataggatg tccgctgagc cccttatata tacgtagtac gaatcaccgg cctggcgttg
      241 tataagcgag accagcgtgg acgaaaaggt aattgtttcc gaatggatcg cgttctctag
      301 aggatccggc aaagcttcgt gtattgtacc ggcccattgt caatcatgca aacttgatat
      361 tatattgaca agagaagaag gcagtttaaa ttaaaactct aaagtagaga gacattaatc
      421 tcagctgaca aggcaggtgg tcacagtaag ttcatttaaa tagttggcca acaatagcct
      481 ttccaagaaa gtatttttgt tccaggtcta tacaaaaata acacacaagc ggccgcaacc
      541 atggcggaca aaaaaccccc ggttcgacag ctccaaaaac tcccaggagt gcggtctgcg
      601 cggagaaatt tatttggtcg agatgataga attgtggacg atttagaaag agacgctgaa
      661 gagttgaaca gtaaagaaca aaaacgattc tcggacacct ggaactttga tgcagccaat
      721 acccaaccat tagatggcag atttgaatgg gacactggcg ttccgctgaa tcaacgaact
      781 cgactattgc atgttccccc gattggtaat gcagattcac ataacgatag tgagcttgta
      841 gaagttaaca actcaacaga atcaaaaaat gaagagttgc cacctacggg gaattcctct
      901 tctgtgggct catgtggatc ttccgaaaat caaagtaaaa gaaggaaact atcttcagaa
      961 tcaactgacg accatgtacc cgctacatcg aagtcgcccc gactgtgacg tcatactgtg
     1021 ccacggaatt ccagctgagc gccggtcgct accattacca gttggtctgg tgtcaaaaat
     1081 aataataacc gggcaggcca tgtctgcccg tatttcgcgt aaggaaatcc attatgtact
     1141 atttaaaaaa cacaaacttt tggatgttcg gtttattctt tttcttttac ttttttatca
     1201 tgggagccta cttcccgttt ttcccgattt ggctacatga catcaaccat atcagcaaaa
     1261 gtgatacggg tattattttt gccgctattt ctctgttctc gctattattc caaccgctgt
     1321 ttggtctgct ttctgacaaa ctcggaactt gtttattgca gcttataatg gttacaaata
     1381 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     1441 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gacaaagtca aagcggccat
     1501 cagatctgcc ggtctcccta tagtgagtcg tattaatttc gataagccag gttaacctgc
     1561 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     1621 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     1681 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1741 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1801 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1861 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1921 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     1981 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2041 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2101 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2161 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2221 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2281 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2341 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2401 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     2461 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     2521 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     2581 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     2641 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     2701 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     2761 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     2821 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     2881 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     2941 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     3001 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     3061 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     3121 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     3181 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     3241 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     3301 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     3361 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     3421 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     3481 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     3541 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     3601 ggcccttacg tattaattaa ggcgcgcc
//
