LOCUS       sequence-207240-        4226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive activation of FGF/MAPK pathway by MEK-S216D, S220E 
            mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Razy-Krajka F, Gravez B, Kaplan N, Racioppi C, Wang W, Christiaen L
  TITLE     An FGF-driven feed-forward circuit patterns the cardiopharyngeal 
            mesoderm in space and time.
  JOURNAL   Elife. 2018 Feb 6;7. pii: 29656. doi: 10.7554/eLife.29656.
  PUBMED    29431097
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-02-6"; volume: "7. pii: 29656. doi"; pages: " 
            10.7554/eLife.29656"
COMMENT     Sequence Label: FoxFTVC+bpFOG_MEK-S216D, S220E
FEATURES             Location/Qualifiers
     source          1..4226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1944..2078
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1981..2000)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2035..2054
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2114..2133)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2115..2133)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2220..2237)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2391..2979)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2471..2490)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3150..3941,3942..4010))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3773..3792
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4011..4115)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4183..4201
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcgttgtatc tttcgttaag tgctggcgtc gagcagggct aacctcattt cgatttgacg
       61 attcgggtaa aaccgagcga tggacaggac ctggaaatgg gaaaggctta ctagaatcag
      121 ggtcgaacga gataagaaga tcaggattcg caaattagca cttggctttt ttacgtagaa
      181 acataggatg tccgctgagc cccttatata tacgtagtac gaatcaccgg cctggcgttg
      241 tataagcgag accagcgtgg acgaaaaggt aattgtttcc gaatggatcg cgttctctag
      301 aggatccggc aaagcttcgt gtattgtacc ggcccattgt caatcatgca aacttgatat
      361 tatattgaca agagaagaag gcagtttaaa ttaaaactct aaagtagaga gacattaatc
      421 tcagctgaca aggcaggtgg tcacagtaag ttcatttaaa tagttggcca acaatagcct
      481 ttccaagaaa gtatttttgt tccaggtcta tacaaaaata acacacaagc ggccgcaacc
      541 atgcctccta aacgtaagtt aaacccgttg aacctaacac ttgaggggag ttcgcctatc
      601 cccaatcagc aggtctctca gttcattaag cagaagcaaa aagtgggagt catggaaaat
      661 gctcagaact ctgacttcac aaagaaagga gaactgggtg ccggaaatgg gggtgttgtt
      721 catcttgtgg tgcacaacgc aactggcttt gtcatggcgc ggaaacttat tcatttggaa
      781 gtgaagcaag ccatcttgaa ccaaatcaca cgagaattgc aggttttgca tgattgtaga
      841 agcccgtaca ttgttgggta ttatgggact ttttacagtg atggggaaat cagtatttgt
      901 atggaaagca tggacgctgg gtcgcttgat ttagtgctga agaaggcgcg aaaaattcct
      961 gagatttact tgggaaaagt gagcaaagct gttatccttg gcctcaagta tttaagagaa
     1021 gaacgtagca tcattcatcg cgatgtaaaa ccatcaaata ttctcgttaa ttcccgagga
     1081 gagattaagt tatgtgattt tggcgtgagc gggcaactga tcgacgatat ggccaacgag
     1141 tttgtaggga caagatcata catggcccca gaacgcttgc aaggatccaa gtatacaatc
     1201 ctttcagata tctggtctct tggtctctca cttattgaaa tggcaattgg tagattccct
     1261 atcccgccac ccacagccag ccaaatagca gccatattca acactgaagt ggcaggggga
     1321 agtggtaaag caccaaaccc acatgatgtt gcacgaccaa tggcaatctt tgagctgctt
     1381 gattacattg tgaatgagcc agcaccgaaa ctcccacaag gaattttcga gaaagatttt
     1441 tgtgatttcg tggctagttg cttgaagaaa gaaccgaaag agcgatcaga tctcggggaa
     1501 ctaatgaagg ctccatttat taaaaatgtt agcttaaccc agtatgagtt tgctaagtgg
     1561 gtttgcagta ctatgggttt gaaagccccg agtccagaca ctgtacctga ttaatatgat
     1621 ggggaattcc agctgagcgc cggtcgctac cattaccagt tggtctggtg tcaaaaataa
     1681 taataaccgg gcaggccatg tctgcccgta tttcgcgtaa ggaaatccat tatgtactat
     1741 ttaaaaaaca caaacttttg gatgttcggt ttattctttt tcttttactt ttttatcatg
     1801 ggagcctact tcccgttttt cccgatttgg ctacatgaca tcaaccatat cagcaaaagt
     1861 gatacgggta ttatttttgc cgctatttct ctgttctcgc tattattcca accgctgttt
     1921 ggtctgcttt ctgacaaact cggaacttgt ttattgcagc ttataatggt tacaaataaa
     1981 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     2041 tgtccaaact catcaatgta tcttatcatg tctggatcga caaagtcaaa gcggccatca
     2101 gatctgccgg tctccctata gtgagtcgta ttaatttcga taagccaggt taacctgcat
     2161 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     2221 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     2281 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     2341 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2401 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2461 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2521 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     2581 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     2641 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     2701 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2761 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     2821 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     2881 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     2941 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     3001 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     3061 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     3121 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     3181 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3241 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     3301 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3361 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3421 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     3481 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3541 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     3601 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     3661 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     3721 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     3781 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     3841 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     3901 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     3961 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     4021 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     4081 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     4141 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     4201 cccttacgta ttaattaagg cgcgcc
//
