LOCUS       sequence-207253-        9665 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human codon optimized MbCpf1(RVR mutant) in mammalian 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Toth E, Czene BC, Kulcsar PI, Krausz SL, Talas A, Nyeste A, Varga E,
            Huszar K, Weinhardt N, Ligeti Z, Borsy AE, Fodor E, Welker E
  TITLE     Mb- and FnCpf1 nucleases are active in mammalian cells: activities 
            and PAM preferences of four wild-type Cpf1 nucleases and of their 
            altered PAM specificity variants.
  JOURNAL   Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 
            10.1093/nar/gky815.
  PUBMED    30239882
REFERENCE   2  (bases 1 to 9665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815."
COMMENT     Sequence Label: pTE3317
FEATURES             Location/Qualifiers
     source          1..9665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5048..5095
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5102..5182
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(5256..5273)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5262..5486
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5532..5960
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5619..5638)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5829..5850
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5969..5989)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5974..6303
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6154..6289
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6216..6235
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6370..7164
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6424..6443)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7034..7053
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7338..7459
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7375..7394)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7429..7448
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7508..7524)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7508..7524)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7521..7543)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7532..7548
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7556..7562,7563..7580,7581..7586))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7601..7622
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7739..7756)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7910..8498)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7990..8009)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8669..9460,9461..9529))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9292..9311
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9530..9634)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgccaccat gctgttccag gactttaccc acctgtatcc
      961 actgtccaag acagtgagat ttgagctgaa gcccatcgat aggaccctgg agcacatcca
     1021 cgccaagaac ttcctgtctc aggacgagac aatggccgat atgcaccaga aggtgaaagt
     1081 gatcctggac gattaccacc gcgacttcat cgccgatatg atgggcgagg tgaagctgac
     1141 caagctggcc gagttctatg acgtgtacct gaagtttcgg aagaacccaa aggacgatga
     1201 gctgcagaag cagctgaagg atctgcaggc cgtgctgaga aaggagatcg tgaagcccat
     1261 cggcaatggc ggcaagtata aggccggcta cgacaggctg ttcggcgcca agctgtttaa
     1321 ggacggcaag gagctgggcg atctggccaa gttcgtgatc gcacaggagg gagagagctc
     1381 cccaaagctg gcccacctgg cccacttcga gaagttttcc acctatttca caggctttca
     1441 cgataaccgg aagaatatgt attctgacga ggataagcac accgccatcg cctaccgcct
     1501 gatccacgag aacctgcccc ggtttatcga caatctgcag atcctgacca caatcaagca
     1561 gaagcactct gccctgtacg atcagatcat caacgagctg accgccagcg gcctggacgt
     1621 gtctctggcc agccacctgg atggctatca caagctgctg acacaggagg gcatcaccgc
     1681 ctacaataca ctgctgggag gaatctccgg agaggcaggc tctcctaaga tccagggcat
     1741 caacgagctg atcaattctc accacaacca gcactgccac aagagcgaga gaatcgccaa
     1801 gctgaggcca ctgcacaagc agatcctgtc cgacggcatg agcgtgtcct tcctgccctc
     1861 taagtttgcc gacgatagcg agatgtgcca ggccgtgaac gagttctatc gccactacgc
     1921 cgacgtgttc gccaaggtgc agagcctgtt cgacggcttt gacgatcacc agaaggatgg
     1981 catctacgtg gagcacaaga acctgaatga gctgtccaag caggccttcg gcgactttgc
     2041 actgctggga cgcgtgctgg acggatacta tgtggatgtg gtgaatccag agttcaacga
     2101 gcggtttgcc aaggccaaga ccgacaatgc caaggccaag ctgacaaagg agaaggataa
     2161 gttcatcaag ggcgtgcact ccctggcctc tctggagcag gccatcgagc actataccgc
     2221 aaggcacgac gatgagagcg tgcaggcagg caagctggga cagtacttca agcacggcct
     2281 ggccggagtg gacaacccca tccagaagat ccacaacaat cacagcacca tcaagggctt
     2341 tctggagagg gagcgccctg caggagagag agccctgcca aagatcaagt ccggcaagaa
     2401 tcctgagatg acacagctga ggcagctgaa ggagctgctg gataacgccc tgaatgtggc
     2461 ccacttcgcc aagctgctga ccacaaagac cacactggac aatcaggatg gcaacttcta
     2521 tggcgagttt ggcgtgctgt acgacgagct ggccaagatc cccaccctgt ataacaaggt
     2581 gagagattac ctgagccaga agcctttctc caccgagaag tacaagctga actttggcaa
     2641 tccaacactg ctgaggggct gggatctgaa cgtggagaag gataggttcg gcgtgatcct
     2701 gcagaaggac ggctgctact atctggccct gctggacaag gcccacaaga aggtgtttga
     2761 taacgcccct aatacaggca agagcatcta tcagaagatg atctataagt acctggaggt
     2821 gaggaagcag ttccccaagg tgttcttttc caaggaggcc atcgccatca actaccaccc
     2881 ttctaaggag ctggtggaga tcaaggacaa gggccggcag agatccgacg atgagcgcct
     2941 gaagctgtat cggtttatcc tggagtgtct gaagatccac cctaagtacg ataagaagtt
     3001 cgagggcgcc atcggcgaca tccagctgtt taagaaggat aagaagggca gagaggtgcc
     3061 aatcagcgag aaggacctgt tcgataagat caacggcatc ttttctagca agcctaagct
     3121 ggagatggag gacttcttta tcggcgagtt caagaggtat aacccaagcc aggacctggt
     3181 ggatcagtat aatatctaca agaagatcga ctccaacgat aatcgcaaga aggagaattt
     3241 ctacaacaat caccccaagt ttaagaagga tctggtgcgg tactattacg agtctatgtg
     3301 caagcacgag gagtgggagg agagcttcga gttttccaag aagctgcagg acatcggctg
     3361 ttacgtggat gtgaacgagc tgtttaccga gatcgagaca cggagactga attataagat
     3421 ctccttctgc aacatcaatg ccgactacat cgatgagctg gtggagcagg gccagctgta
     3481 tctgttccag atctacaaca aggacttttc cccaaaggcc cacggcaagc ccaatctgca
     3541 caccctgtac ttcaaggccc tgttttctga ggacaacctg gccgatccta tctataagct
     3601 gaatggcgag gcccagatct tctacagaaa ggcctccctg gacatgaacg agacaacaat
     3661 ccacagggcc ggcgaggtgc tggagaacaa gaatcccgat aatcctaaga agagacagtt
     3721 cgtgtacgac atcatcaagg ataagaggta cacacaggac aagttcatgc tgcacgtgcc
     3781 aatcaccatg aactttggcg tgcagggcat gacaatcaag gagttcaata agaaggtgaa
     3841 ccagtctatc cagcagtatg acgaggtgaa cgtgatcggc atcgatcggg gcgagagaca
     3901 cctgctgtac ctgaccgtga tcaatagcaa gggcgagatc ctggagcagt gttccctgaa
     3961 cgacatcacc acagcctctg ccaatggcac acagatgacc acaccttacc acaagatcct
     4021 ggataagagg gagatcgagc gcctgaacgc ccgggtggga tggggcgaga tcgagacaat
     4081 caaggagctg aagtctggct atctgagcca cgtggtgcac cagatcagcc agctgatgct
     4141 gaagtacaac gccatcgtgg tgctggagga cctgaatttc ggctttaaga ggggccgctt
     4201 taaggtggag aagcagatct atcagaactt cgagaatgcc ctgatcaaga agctgaacca
     4261 cctggtgctg aaggacaagg ccgacgatga gatcggctct tacaagaatg ccctgcagct
     4321 gaccaacaat ttcacagatc tgaagagcat cggcaagcag accggcttcc tgttttatgt
     4381 gcccgcctgg aacacctcta agatcgaccc tgagacaggc tttgtggatc tgctgaagcc
     4441 aagatacgag aacatcgccc agagccaggc cttctttggc aagttcgaca agatctgcta
     4501 taatgccgac aaggattact tcgagtttca catcgactac gccaagttta ccgataaggc
     4561 caagaatagc cgccagatct ggacaatctg ttcccacggc gacaagcggt acgtgtacga
     4621 taagacagcc aaccagaata agggcgccgc caagggcatc aacgtgaatg atgagctgaa
     4681 gtccctgttc gcccgccacc acatcaacga gaagcagccc aacctggtca tggacatctg
     4741 ccagaacaat gataaggagt ttcacaagtc tctgatgtac ctgctgaaaa ccctgctggc
     4801 cctgcggtac agcaacgcct cctctgacga ggatttcatc ctgtcccccg tggcaaacga
     4861 cgagggcgtg ttctttaata gcgccctggc cgacgataca cagcctcaga atgccgatgc
     4921 caacggcgcc taccacatcg ccctgaaggg cctgtggctg ctgaatgagc tgaagaactc
     4981 cgacgatctg aacaaggtga agctggccat cgacaatcag acctggctga atttcgccca
     5041 gaacaggaaa aggccggcgg ccacgaaaaa ggccggccag gcaaaaaaga aaaagggatc
     5101 ctacccatac gatgttccag attacgctta tccctacgac gtgcctgatt atgcataccc
     5161 atatgatgtc cccgactatg cctaagaatt ctgcagatat ccagcacagt ggcggccgct
     5221 cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     5281 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     5341 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     5401 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     5461 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     5521 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     5581 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     5641 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     5701 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     5761 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     5821 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     5881 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     5941 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     6001 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     6061 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     6121 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     6181 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     6241 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     6301 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
     6361 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     6421 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     6481 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     6541 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     6601 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     6661 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     6721 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     6781 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     6841 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     6901 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     6961 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     7021 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     7081 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     7141 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     7201 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     7261 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     7321 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7381 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     7441 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     7501 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     7561 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     7621 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     7681 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     7741 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     7801 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     7861 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     7921 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7981 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8041 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     8101 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8161 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8221 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8281 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8341 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8401 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     8461 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8521 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     8581 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     8641 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     8701 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     8761 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8821 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     8881 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     8941 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     9001 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     9061 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     9121 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     9181 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     9241 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     9301 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     9361 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     9421 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     9481 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9541 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9601 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     9661 acgtc
//
