LOCUS       sequence-207259-        9389 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human codon optimized FnCpf1(RR mutant) in mammalian 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9389)
  AUTHORS   Toth E, Czene BC, Kulcsar PI, Krausz SL, Talas A, Nyeste A, Varga E,
            Huszar K, Weinhardt N, Ligeti Z, Borsy AE, Fodor E, Welker E
  TITLE     Mb- and FnCpf1 nucleases are active in mammalian cells: activities 
            and PAM preferences of four wild-type Cpf1 nucleases and of their 
            altered PAM specificity variants.
  JOURNAL   Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 
            10.1093/nar/gky815.
  PUBMED    30239882
REFERENCE   2  (bases 1 to 9389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815."
COMMENT     Sequence Label: pTE3316
FEATURES             Location/Qualifiers
     source          1..9389
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        72..451
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        452..655
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     606..626
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     700..719
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        700..718
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4666..4713
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4720..4800
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(4874..4891)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4880..5104
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5150..5578
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5237..5256)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5447..5468
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5587..5607)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5592..5921
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5772..5907
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5834..5853
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5988..6782
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6042..6061)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6652..6671
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6956..7077
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6993..7012)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7047..7066
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7126..7142)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7126..7142)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7139..7161)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7150..7166
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7174..7180,7181..7198,7199..7204))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7219..7240
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7357..7374)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7528..8116)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7608..7627)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8287..9078,9079..9147))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8910..8929
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9148..9252)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9327..9346)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
       61 cgtacaaaaa agacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      121 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      181 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      241 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      301 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      361 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      421 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      481 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      541 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      601 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg
      661 gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
      721 gacccaagct ggctagcgtt taaacttaag cttggtaccg ccaccatgag catctaccag
      781 gagttcgtca acaagtattc actgagtaag acactgcggt tcgagctgat cccacagggc
      841 aagacactgg agaacatcaa ggcccgaggc ctgattctgg acgatgagaa gcgggcaaaa
      901 gactataaga aagccaagca gatcattgat aaataccacc agttctttat cgaggaaatt
      961 ctgagctccg tgtgcatcag tgaggatctg ctgcagaatt actcagacgt gtacttcaag
     1021 ctgaagaaga gcgacgatga caacctgcag aaggacttta agtccgccaa ggacaccatc
     1081 aagaaacaga ttagcgagta catcaaggac tccgaaaagt ttaaaaatct gttcaaccag
     1141 aatctgatcg atgctaagaa aggccaggag tccgacctga tcctgtggct gaaacagtct
     1201 aaggacaatg ggattgaact gttcaaggct aactccgata tcactgatat tgacgaggca
     1261 ctggaaatca tcaagagctt caagggatgg accacatact ttaaaggctt ccacgagaac
     1321 cgcaagaacg tgtactccag caacgacatt cctacctcca tcatctaccg aatcgtcgat
     1381 gacaatctgc caaagttcct ggagaacaag gccaaatatg aatctctgaa ggacaaagct
     1441 cccgaggcaa ttaattacga acagatcaag aaagatctgg ctgaggaact gacattcgat
     1501 atcgactata agactagcga ggtgaaccag agggtctttt ccctggacga ggtgtttgaa
     1561 atcgccaatt tcaacaatta cctgaaccag tccggcatta ctaaattcaa taccatcatt
     1621 ggcgggaagt ttgtgaacgg ggagaatacc aagcgcaagg gaattaacga atacatcaat
     1681 ctgtatagcc agcagatcaa cgacaaaact ctgaagaaat acaagatgtc tgtgctgttc
     1741 aaacagatcc tgagtgatac cgagtccaag tcttttgtca ttgataaact ggaagatgac
     1801 tcagacgtgg tcactaccat gcagagcttt tatgagcaga tcgccgcttt caagacagtg
     1861 gaggaaaaat ctattaagga aactctgagt ctgctgttcg atgacctgaa agcccagaag
     1921 ctggacctga gtaagatcta cttcaaaaac gataagagtc tgacagacct gtcacagcag
     1981 gtgtttgatg actattccgt gattgggacc gccgtcctgg agtacattac acagcagatc
     2041 gctccaaaga acctggataa tccctctaag aaagagcagg aactgatcgc taagaaaacc
     2101 gagaaggcaa aatatctgag tctggaaaca attaagctgg cactggagga gttcaacaag
     2161 cacagggata ttgacaaaca gtgccgcttt gaggaaatcc tggccaactt cgcagccatc
     2221 cccatgattt ttgatgagat cgcccagaac aaagacaatc tggctcagat cagtattaag
     2281 taccagaacc agggcaagaa agacctgctg caggcttcag cagaagatga cgtgaaagcc
     2341 atcaaggatc tgctggacca gaccaacaat ctgctgcaca agctgaaaat cttccatatt
     2401 agtcagtcag aggataaggc taatatcctg gataaagacg aacacttcta cctggtgttc
     2461 gaggaatgtt acttcgagct ggcaaacatt gtccccctgt ataacaagat taggaactac
     2521 atcacacaga agccttactc tgacgagaag tttaaactga acttcgaaaa tagtacgcta
     2581 gccagggggt gggataagaa caaggagcct gacaacacag ctatcctgtt catcaaggat
     2641 gacaagtact atctgggagt gatgaataag aaaaacaata agatcttcga tgacaaagcc
     2701 attaaggaga acaaagggga aggatacaag aaaatcgtgt ataagctgct gcccggcgca
     2761 aataagatgc tgcctagggt gttcttcagc gccaagagta tcaaattcta caacccatcc
     2821 gaggacatcc tgcggattag aaatcactca acacatacta agaacgggag cccccagaag
     2881 ggatatgaga aatttgagtt caacatcgag gattgcagga agtttattga cttctacaag
     2941 cagagcatct ccaaacaccc tgaatggaag gattttggct tccggttttc cgacacacag
     3001 agatataact ctatcgacga gttctaccgc gaggtggaaa atcaggggta taagctgact
     3061 tttgagaaca tttctgaaag ttacatcgac agcgtggtca atcagggaaa gctgtacctg
     3121 ttccagatct ataacaaaga tttttcagca tacagcaagg gcagaccaaa cctgcataca
     3181 ctgtactgga aggccctgtt cgatgagagg aatctgcagg acgtggtcta taaactgaac
     3241 ggagaggccg aactgtttta ccggaagcag tctattccta agaaaatcac tcacccagct
     3301 aaggaggcca tcgctaacaa gaacaaggac aatcctaaga aagagagcgt gttcgaatac
     3361 gatctgatta aggacaagcg gttcaccgaa gataagttct ttttccattg tccaatcacc
     3421 attaacttca agtcaagcgg cgctaacaag ttcaacgacg agatcaatct gctgctgaag
     3481 gaaaaagcaa acgatgtgca catcctgagc attgaccgag gagagcggca tctggcctac
     3541 tataccctgg tggatggcaa agggaatatc attaagcagg atacattcaa catcattggc
     3601 aatgaccgga tgaaaaccaa ctaccacgat aaactggctg caatcgagaa ggatagagac
     3661 tcagctagga aggactggaa gaaaatcaac aacattaagg agatgaagga aggctatctg
     3721 agccaggtgg tccatgagat tgcaaagctg gtcatcgaat acaatgccat tgtggtgttc
     3781 gaggatctga acttcggctt taagaggggg cgctttaagg tggaaaaaca ggtctatcag
     3841 aagctggaga aaatgctgat cgaaaagctg aattacctgg tgtttaaaga taacgagttc
     3901 gacaagaccg gaggcgtcct gagagcctac cagctgacag ctccctttga aactttcaag
     3961 aaaatgggaa aacagacagg catcatctac tatgtgccag ccggattcac ttccaagatc
     4021 tgccccgtga ccggctttgt caaccagctg taccctaaat atgagtcagt gagcaagtcc
     4081 caggaatttt tcagcaagtt cgataagatc tgttataatc tggacaaggg gtacttcgag
     4141 ttttccttcg attacaagaa cttcggcgac aaggccgcta aggggaaatg gaccattgcc
     4201 tccttcggat ctcgcctgat caactttcga aattccgata aaaaccacaa ttgggacact
     4261 agggaggtgt acccaaccaa ggagctggaa aagctgctga aagactactc tatcgagtat
     4321 ggacatggcg aatgcatcaa ggcagccatc tgtggcgaga gtgataagaa atttttcgcc
     4381 aagctgacct cagtgctgaa tacaatcctg cagatgcgga actcaaagac cgggacagaa
     4441 ctggactatc tgattagccc cgtggctgat gtcaacggaa acttcttcga cagcagacag
     4501 gcacccaaaa atatgcctca ggatgcagac gccaacgggg cctaccacat cgggctgaag
     4561 ggactgatgc tgctgggccg gatcaagaac aatcaggagg ggaagaagct gaacctggtc
     4621 attaagaacg aggaatactt cgagtttgtc cagaatagaa ataacaaaag gccggcggcc
     4681 acgaaaaagg ccggccaggc aaaaaagaaa aagggatcct acccatacga tgttccagat
     4741 tacgcttatc cctacgacgt gcctgattat gcatacccat atgatgtccc cgactatgcc
     4801 taagaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg gcccgtttaa
     4861 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     4921 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     4981 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     5041 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     5101 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     5161 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     5221 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     5281 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     5341 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     5401 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     5461 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     5521 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     5581 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     5641 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     5701 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     5761 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     5821 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     5881 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     5941 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     6001 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     6061 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     6121 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     6181 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     6241 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     6301 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     6361 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     6421 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     6481 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     6541 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     6601 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     6661 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     6721 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     6781 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     6841 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     6901 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     6961 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7021 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7081 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     7141 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7201 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7261 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     7321 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7381 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7441 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7501 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7561 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7621 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7681 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7741 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7801 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7861 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7921 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7981 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8041 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8101 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     8161 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8221 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8281 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8341 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     8401 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     8461 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     8521 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8581 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8641 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8701 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8761 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8821 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8881 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8941 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     9001 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     9061 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     9121 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     9181 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     9241 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
     9301 cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     9361 tatctgctcc ctgcttgtgt gttggaggt
//
