LOCUS       sequence-207262-        6218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent protein for superresolution imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Wang S, Moffitt JR, Dempsey GT, Xie XS, Zhuang X
  TITLE     Characterization and development of photoactivatable fluorescent 
            proteins for single-molecule-based superresolution imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jun 10;111(23):8452-7. doi: 
            10.1073/pnas.1406593111. Epub 2014 May 27.
  PUBMED    24912163
REFERENCE   2  (bases 1 to 6218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1406593111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-06-10- 10"; volume: "111"; issue: "23"; pages: "8452-7"
COMMENT     Sequence Label: pmMaple3-CAM
FEATURES             Location/Qualifiers
     source          1..6218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        17..301
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     224..243
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     protein_bind    1071..1104
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        1164..1266
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(1333..1352)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    1915..1948
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(2017..2034)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2187..2273
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2365..2392
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        2411..2502
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2503..2571,2572..3363)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2721..2740)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3405..3860
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3492..3511)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3702..3723
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      3971..4559
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4460..4479
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4713..4730
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4745..4885
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4811..4830)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4971..4993
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5131..5150
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(5330..6208)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc
       61 gtcttttact ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa aagcattctg
      121 taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc
      181 agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta
      241 tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca
      301 tacccgtttt tttgggctag caggaggaat tcaccatgtc tggtggcggc ggttctaaag
      361 ttagcaaggg cgaagaaacc atcatgagcg tgatcaagcc ggacatgaag atcaagctgc
      421 gcatggaggg caacgtgaac ggccatgcct ttgtgatcga gggcgagggc agcggtaagc
      481 cgtttgaggg catccagacc atcgacctgg aggttaagga aggcgcaccg ctgccgtttg
      541 cctacgacat cctgaccacc gcattccact acggcaaccg cgtgttcacc aagtacccgc
      601 gcaagatccc ggactacttc aagcagagct tcccggaggg ctacagttgg gaacgcagca
      661 tgacctacga ggacggcggt atctgcaacg ccaccaacga catcaccatg gaagaagata
      721 gcttcatcaa caagatccac ttcaagggca caaacttccc gccgaatggt ccggttatgc
      781 agaagcgcac cgttggctgg gaggtgagca ccgagaagat gtatgtgcgc gacggtgtgc
      841 tgaagggcga cgtgaagatg aagctgctgc tgaagggtgg cagccactac cgctgcgact
      901 tccgcaccac ctacaaagtt aagcaaaagg cagtgaagtt accgaaggcc cacttcgtgg
      961 accaccgcat cgaaatcctg agccacgaca aggactataa caaagtgaag ctgtacgagc
     1021 acgccgtggc ccgtaacagc accgacagca tggatgagct gtacaaataa gaagttccta
     1081 ttctctagaa agtataggaa cttcactggt gtccctgttg ataccgggaa gccctgggcc
     1141 aacttttggc gaaaatgaga cgttgatcgg cacgtaagag gttccaactt tcaccataat
     1201 gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa
     1261 gctaaaatgg agaaaaagat cactggatat accaccgttg atatatccca atggcatcgt
     1321 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
     1381 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
     1441 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa
     1501 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
     1561 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
     1621 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
     1681 attgagaata tgtttttcgt ctcagccaat ccctgggtgg gtttcaccag ttttgattta
     1741 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg
     1801 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc
     1861 ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcaggaagtt
     1921 cctattctct agaaagtata ggaacttcga agcagctcca gcctacaatt taataagaaa
     1981 gcttggctgt tttggcggat gagagaagat tttcagcctg atacagatta aatcagaacg
     2041 cagaagcggt ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga
     2101 ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca
     2161 tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     2221 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg
     2281 gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat
     2341 aaactgccag gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc
     2401 tacaaactct tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     2461 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     2521 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     2581 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     2641 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     2701 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     2761 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     2821 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     2881 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     2941 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     3001 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca
     3061 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     3121 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     3181 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     3241 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     3301 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     3361 taactgtcag accaagttta ctcatatata ctttagattg atttacgcgc cctgtagcgg
     3421 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     3481 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     3541 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     3601 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
     3661 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     3721 tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat
     3781 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     3841 aatattaacg tttacaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     3901 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     3961 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4021 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     4081 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     4141 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4201 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4261 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4321 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4381 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4441 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4501 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4561 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4621 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     4681 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     4741 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     4801 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
     4861 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
     4921 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     4981 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagaagg agatggcgcc
     5041 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag
     5101 cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac
     5161 cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tctgctcatg
     5221 tttgacagct tatcatcgat gcataatgtg cctgtcaaat ggacgaagca gggattctgc
     5281 aaaccctatg ctactccgtc aagccgtcaa ttgtctgatt cgttaccaat tatgacaact
     5341 tgacggctac atcattcact ttttcttcac aaccggcacg gaactcgctc gggctggccc
     5401 cggtgcattt tttaaatacc cgcgagaaat agagttgatc gtcaaaacca acattgcgac
     5461 cgacggtggc gataggcatc cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt
     5521 ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg gcggaaaaga tgtgacagac
     5581 gcgacggcga caagcaaaca tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca
     5641 ggtgatcgct gatgtactga caagcctcgc gtacccgatt atccatcggt ggatggagcg
     5701 actcgttaat cgcttccatg cgccgcagta acaattgctc aagcagattt atcgccagca
     5761 gctccgaata gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga
     5821 aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt attggcaaat attgacggcc
     5881 agttaagcca ttcatgccag taggcgcgcg gacgaaagta aacccactgg tgataccatt
     5941 cgcgagcctc cggatgacga ccgtagtgat gaatctctcc tggcgggaac agcaaaatat
     6001 cacccggtcg gcaaacaaat tctcgtccct gatttttcac caccccctga ccgcgaatgg
     6061 tgagattgag aatataacct ttcattccca gcggtcggtc gataaaaaaa tcgagataac
     6121 cgttggcctc aatcggcgtt aaacccgcca ccagatgggc attaaacgag tatcccggca
     6181 gcaggggatc attttgcgct tcagccatac ttttcata
//
