LOCUS       sequence-207264-        6218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent protein for superresolution imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Wang S, Moffitt JR, Dempsey GT, Xie XS, Zhuang X
  TITLE     Characterization and development of photoactivatable fluorescent 
            proteins for single-molecule-based superresolution imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jun 10;111(23):8452-7. doi: 
            10.1073/pnas.1406593111. Epub 2014 May 27.
  PUBMED    24912163
REFERENCE   2  (bases 1 to 6218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1406593111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-06-10- 10"; volume: "111"; issue: "23"; pages: "8452-7"
COMMENT     Sequence Label: pmMaple2-CAM
FEATURES             Location/Qualifiers
     source          1..6218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        13..297
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     220..239
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     protein_bind    1067..1100
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        1160..1262
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(1329..1348)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    1911..1944
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(2013..2030)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2183..2269
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2361..2388
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        2407..2498
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2499..2567,2568..3359)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2717..2736)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3401..3856
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3488..3507)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3698..3719
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      3967..4555
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4456..4475
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4709..4726
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4741..4881
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4807..4826)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4967..4989
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5127..5146
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(5326..6204)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt cactgcgtct
       61 tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc attctgtaac
      121 aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat cacggcagaa
      181 aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca tttttatcca
      241 taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt tctccatacc
      301 cgtttttttg ggctagcagg aggaattcac catgtctggt ggcggcggtt ctaaagttag
      361 caagggcgaa gaaaccatca tgagcgtgat caagccggac atgaagatca agctgcgcat
      421 ggagggcaac gtgaacggcc atgcctttgt gatcgagggc gagggcagcg gtaagccgtt
      481 tgagggcatc cagaccatcg acctggaggt taaggaaggc gcaccgctgc cgtttgccta
      541 cgacatcctg accaccgcat tccactacgg caaccgcgtg ttcaccaagt acccggagga
      601 catcccggac tacttcaagc agagcttccc ggagggctac agttgggaac gcagcatgac
      661 ctacgaggac ggcggtatct gcaacgccac caacgacatc accatggaag aagatagctt
      721 catcaacaag atccacttca agggcacaaa cttcccgccg aatggtccgg ttatgcagaa
      781 gcgcaccgtt ggctgggagg tgagcaccga gaagatgtat gtgcgcgacg gtgtgctgaa
      841 gggcgacgtg aagatgaagc tgctgctgaa gggtggcagc cactaccgct gcgacttccg
      901 caccacctac aaagttaagc aaaaggcagt gaagttaccg gacgcccact tcgtggacca
      961 ccgcatcgaa atcctgagcc acgacaagga ctataacaaa gtgaagctgt acgagcacgc
     1021 cgtggcccgt aacagcaccg acagcatgga tgagctgtac aaataagaag ttcctattct
     1081 ctagaaagta taggaacttc actggtgtcc ctgttgatac cgggaagccc tgggccaact
     1141 tttggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac cataatgaaa
     1201 taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta
     1261 aaatggagaa aaagatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag
     1321 aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg
     1381 atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta
     1441 ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg
     1501 gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg
     1561 aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat
     1621 attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg
     1681 agaatatgtt tttcgtctca gccaatccct gggtgggttt caccagtttt gatttaaacg
     1741 tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag
     1801 gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc
     1861 atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag gaagttccta
     1921 ttctctagaa agtataggaa cttcgaagca gctccagcct acaatttaat aagaaagctt
     1981 ggctgttttg gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga
     2041 agcggtctga taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc
     2101 atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg
     2161 agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     2221 tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc
     2281 ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac
     2341 tgccaggcat caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca
     2401 aactcttttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     2461 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     2521 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     2581 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     2641 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     2701 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc
     2761 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     2821 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     2881 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     2941 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     3001 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt
     3061 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     3121 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     3181 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     3241 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     3301 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     3361 tgtcagacca agtttactca tatatacttt agattgattt acgcgccctg tagcggcgca
     3421 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     3481 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     3541 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     3601 cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     3661 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     3721 acaacactca accctatctc gggctattct tttgatttat aagggatttt gccgatttcg
     3781 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     3841 ttaacgttta caatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     3901 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     3961 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     4021 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     4081 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     4141 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4201 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     4261 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4321 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4381 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4441 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4501 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4561 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     4621 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     4681 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     4741 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     4801 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
     4861 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     4921 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     4981 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agaaggagat ggcgcccaac
     5041 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg
     5101 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
     5161 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatctg ctcatgtttg
     5221 acagcttatc atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac
     5281 cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac
     5341 ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt
     5401 gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac
     5461 ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc
     5521 ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga
     5581 cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg
     5641 atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc
     5701 gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc
     5761 cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg
     5821 cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt
     5881 aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg
     5941 agcctccgga tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc
     6001 cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag
     6061 attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt
     6121 ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag
     6181 gggatcattt tgcgcttcag ccatactttt catactcc
//
