LOCUS       sequence-207275-        4246 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cGAL-C driver under the control of C. elegans myo-2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Wang H, Liu J, Yuet KP, Hill AJ, Sternberg PW
  TITLE     Split cGAL, an intersectional strategy using a split intein for 
            refined spatiotemporal transgene control in Caenorhabditiselegans.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Mar 26. pii: 1720063115. doi: 
            10.1073/pnas.1720063115.
  PUBMED    29581308
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2018 Mar 26. pii: 1720063115. doi: 
            10.1073/pnas.1720063115."
COMMENT     Sequence Label: pAH36 (Pmyo-2::cGAL-C::let-858 3'UTR)
FEATURES             Location/Qualifiers
     source          1..4246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1268..1417
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        1955..2059
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2060..2128,2129..2920)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2278..2297)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3091..3679
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3580..3599
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3833..3850
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3967..3988
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4003..4008,4009..4026,4027..4033)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4041..4057
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4046..4068
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4065..4081
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4065..4081
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gtagtatcct ttgctttaaa tgtccataaa actaatttta taatcaataa aacaacgttt
       61 gtaaatcaac tgagtttaca agtagagaca ttgagggata ctttcactat gctaaagtga
      121 taatcgacca aataataact tcactttggt atttattcct gtcttataaa tgttatgtat
      181 gaattaaatt catatgcata tggctcactc tgacaatttt taataatctt ccagatcaat
      241 attgactacc gatgcgggtg gtcttttgct ttgaattctg ctgaacttta caccccgaac
      301 agcaatgtgt gcttcagcct aaaaaaaagt aagtgtgtta atcagtgccc ccgattcttc
      361 attttttgcc cctctctccc gtttcgtcgg caaaagaaga gaaaataaag ataagtctca
      421 agataggttg gtaatcgcta aagtggttgt gtggataaga gtagcaaaat ggcaggaaga
      481 gcactttgcg cgcacacact gtactcattg ttctggataa aattctctcg ttgtttgccg
      541 tcggatgtct gcctctctgc attgagccgg cttcttcact atctttagtt aacctaaaat
      601 gccgtttctt ttctcgtatc cccactatcc cgttgaggtt ctctgctctc ttcgctccct
      661 accgccagcg agcaactatc cgtgggggcg ccttgctcgg aagatggggg ggaagaaaga
      721 agatttttgc tatttgcact tgagaaagag acttttcctg cgtcgatggt tagagaacag
      781 tgtgcagaca cttttcagct acctagaatt acaattggat atccccgcct cccaatccac
      841 ccacccaggg aaaaagaagg gctcgccgaa aatcaaagtt atctccaggc tcgcgcatcc
      901 caccgagcgg ttgacttctc tccaccactt ttcattttaa ccctcgatcg tcagacacag
      961 aaggcgcgcc tctagaggat ccccgggatt ggccaaagga cccaaaggta tgtttcgaat
     1021 gatactaaca taacatagaa cattttcagg aggacccttg gctaggatgg gccctaaaaa
     1081 gaagcgtaaa gctagcatga tgttgaagaa gattctcaaa attgaagaac tggatgagcg
     1141 tgagctcatc gacatcgagg tctccggaaa ccacctcttc tacgccaacg acatcctcac
     1201 ccacaactcc tcctccgatg tactgcagtc tggtggcgga ggggctccta ggggttccgg
     1261 acgggctgac gcattggacg attttgatct ggatatgctg ggaagtgacg ccctcgatga
     1321 ttttgacctt gacatgcttg gttcggatgc ccttgatgac tttgacctcg acatgctcgg
     1381 cagtgacgcc cttgatgatt tcgacctgga catgctgatt aactaaggta ccgaattcgc
     1441 tagccggcca tacaagtaat ccggatgatc gacgccaacg tcgttgaatt ttcaaatttt
     1501 aaatactgaa tatttgtttt ttttcctatt atttatttat tctctttgtg ttttttttct
     1561 tgctttctaa aaaattaatt caatccaaat ctaaacattt ttttttctct ttccgtctcc
     1621 caattcgtat tccgctcctc tcatctgaac acaatgtgca agtttattta tcttctcgct
     1681 ttcatttcat taggacgtgg ggggaattgg tggaaggggg aaacacacaa aaggatgatg
     1741 gaaatgaaat aaggacacac aatatgcaac aacattcaat tcagaaatat ggaggaaggt
     1801 ttaaaagaaa acataaaaat atatagagga ggaaggaaaa ctagtaaaaa ataagcaaag
     1861 aaattaggcg aacgatgaga attgtcctcg cttgggccca aaaggccttt tttttttggc
     1921 gccgacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     1981 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     2041 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     2101 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     2161 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     2221 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     2281 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     2341 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     2401 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     2461 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     2521 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     2581 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     2641 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     2701 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     2761 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     2821 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     2881 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     2941 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     3001 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     3061 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3121 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3181 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     3241 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3301 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3361 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3421 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3481 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     3541 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3601 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3661 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3721 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     3781 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     3841 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     3901 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     3961 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     4021 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     4081 ccatgattac gccaagctgt aagtttaaac atgatcttac taactaacta ttctcattta
     4141 aattttcaga gcttaaaaat ggctgaaatc actcacaacg atggatacgc taacaacttg
     4201 gaaatgaaat aagcttgcat gcgcggccgc actgactggg ccggcc
//
