LOCUS       sequence-20729-e         634 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG305GAL-ccdB-TAP
FEATURES             Location/Qualifiers
     source          1..634
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(15..139)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(147..163)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(147..163)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(178..619)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(184..207)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    502..619
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 ctaatagcgg ccgccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt
       61 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag
      121 cttttttgta caaacttgtg atgggggatc cactagttct agaatccggg gttttttctc
      181 cttgacgtta aagtatagag gtatattaac aattttttgt tgatactttt attacatttg
      241 aataagaagt aatacaaacc gaaaatgttg aaagtattag ttaaagtggt tatgcagttt
      301 ttgcatttat atatctgtta atagatcaaa aatcatcgct tcgctgatta attaccccag
      361 aaataaggct aaaaaactaa tcgcattatc atcctatggt tgttaatttg attcgttcat
      421 ttgaaggttt gtggggccag gttactgcca atttttcctc ttcataacca taaaagctag
      481 tattgtanaa tctttattgt tcggagcagt gcggcgcgag gcacatctgc gtttcaggaa
      541 cgcgaccggt gaagacgagg acgcacggag gagagtcttc cttcggaggg ctgtcacccg
      601 ctcggcggct tctaatccgt actagagctc caat
//
