LOCUS       sequence-207297-        2892 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of mutant human alpha synuclein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2892)
  TITLE     Lashuel Lab plasmids
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-7 asyn S129W
FEATURES             Location/Qualifiers
     source          1..2892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      14..602
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     503..522
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     756..773
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1008..1030
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1095..1114
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1095..1113
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1145..1167
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             complement(join(1726..2517,2518..2586))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2349..2368
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2587..2691)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2759..2777
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
       61 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      121 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
      181 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      241 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      301 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      361 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      421 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      481 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      541 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      601 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      661 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      721 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      781 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatag
      841 gaagatcttc cggaagatct tcctatggtg cactctcagt acaatctgct ctgatgcgct
      901 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
      961 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     1021 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cccagcgatt cgaacttctg
     1081 atagacttcg aaattaatac gactcactat agggagacca caacggtttc cctctagaaa
     1141 taattttgtt taactttaag aaggagatat acatatggat gtattcatga aaggactttc
     1201 aaaggccaag gagggagttg tggctgctgc tgagaaaacc aaacagggtg tggcagaagc
     1261 agcaggaaag acaaaagagg gtgttctcta tgtaggctcc aaaaccaagg agggagtggt
     1321 gcatggtgtg gcaacagtgg ctgagaagac caaagagcaa gtgacaaatg ttggaggagc
     1381 agtggtgacg ggtgtgacag cagtagccca gaagacagtg gagggagcag ggagcattgc
     1441 agcagccact ggctttgtca aaaaggacca gttgggcaag aatgaagaag gagccccaca
     1501 ggaaggaatt ctggaagata tgcctgtgga tcctgacaat gaggcttatg aaatgccttg
     1561 ggaggaaggg tatcaagact acgaacctga agcctaagaa atatctttgc tcccagtttc
     1621 ttgagatctg ctgacagatg ttccatccaa gcttatcgat gataagctgt caaacatgag
     1681 aattaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     1741 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     1801 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     1861 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     1921 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     1981 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     2041 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     2101 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     2161 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     2221 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     2281 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     2341 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     2401 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     2461 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     2521 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     2581 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     2641 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     2701 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     2761 taggcgtatc acgaggccct ttcgtcttca agaataaaag gatctaggtg aagatccttt
     2821 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     2881 ccgtagaaaa ga
//
