LOCUS       sequence-207307-        9750 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector for catalytically inactive Cpf1 from 
            Lachnospiraceae bacterium (dLbCpf1) fused to three DmrA domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9750)
  AUTHORS   Tak YE, Kleinstiver BP, Nunez JK, Hsu JY, Horng JE, Gong J, Weissman
            JS, Joung JK
  TITLE     Inducible and multiplex gene regulation using CRISPR-Cpf1-based 
            transcription factors.
  JOURNAL   Nat Methods. 2017 Oct 30. doi: 10.1038/nmeth.4483.
  PUBMED    29083402
REFERENCE   2  (bases 1 to 9750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Oct 30. doi: 10.1038/nmeth.4483."
COMMENT     Sequence Label: JG693: CAG-human
            dLbCpf1(D832A)-NLS-3xHA-3xFLA...
FEATURES             Location/Qualifiers
     source          1..9750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..662
                     /label=chicken beta-actin promoter
     intron          663..1671
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1595..1617
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1679..1698
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1770..5453
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIARGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             5454..5501
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5508..5588
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             5595..5660
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5688..6008
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             6030..6350
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             6360..6686
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLEG"
     primer_bind     complement(6772..6791)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6837..6892
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6891..6910)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7253..7269)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7253..7269)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7266..7288)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7277..7293
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7301..7307,7308..7325,7326..7331))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7346..7367
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7426..7621
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7472..7607
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7627..7761
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7664..7683)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7718..7737
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7829..7846)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8000..8588)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8080..8099)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8759..9550,9551..9619))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9382..9401
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9620..9724)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catgggtcga ggtgagcccc acgttctgct tcactctccc
      421 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
      481 agcgatgggg gcgggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgc tgcgttgcct tcgccccgtg ccccgctccg cgccgcctcg cgccgcccgc
      721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      781 cgggctgtaa ttagcgcttg gtttaatgac ggctcgtttc ttttctgtgg ctgcgtgaaa
      841 gccttaaagg gctccgggag ggccctttgt gcggggggga gcggctcggg gggtgcgtgc
      901 gtgtgtgtgt gcgtggggag cgccgcgtgc ggcccgcgct gcccggcggc tgtgagcgct
      961 gcgggcgcgg cgcggggctt tgtgcgctcc gcgtgtgcgc gaggggagcg cggccggggg
     1021 cggtgccccg cggtgcgggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     1081 gtgggggggt gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc
     1141 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg
     1201 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
     1261 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggccccggag cgccggcggc
     1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1381 ggacttcctt tgtcccaaat ctggcggagc cgaaatctgg gaggcgccgc cgcaccccct
     1441 ctagcgggcg cgggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct
     1501 tcgtgcgtcg ccgcgccgcc gtccccttct ccatctccag cctcggggct gccgcagggg
     1561 gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc
     1621 ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc
     1681 aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattctgcag tcgacggtac
     1741 cgcgggcccg ggatccaccg gtcgccacca tgagcaagct ggagaagttt acaaactgct
     1801 actccctgtc taagaccctg aggttcaagg ccatccctgt gggcaagacc caggagaaca
     1861 tcgacaataa gcggctgctg gtggaggacg agaagagagc cgaggattat aagggcgtga
     1921 agaagctgct ggatcgctac tatctgtctt ttatcaacga cgtgctgcac agcatcaagc
     1981 tgaagaatct gaacaattac atcagcctgt tccggaagaa aaccagaacc gagaaggaga
     2041 ataaggagct ggagaacctg gagatcaatc tgcggaagga gatcgccaag gccttcaagg
     2101 gcaacgaggg ctacaagtcc ctgtttaaga aggatatcat cgagacaatc ctgccagagt
     2161 tcctggacga taaggacgag atcgccctgg tgaacagctt caatggcttt accacagcct
     2221 tcaccggctt ctttgataac agagagaata tgttttccga ggaggccaag agcacatcca
     2281 tcgccttcag gtgtatcaac gagaatctga cccgctacat ctctaatatg gacatcttcg
     2341 agaaggtgga cgccatcttt gataagcacg aggtgcagga gatcaaggag aagatcctga
     2401 acagcgacta tgatgtggag gatttctttg agggcgagtt ctttaacttt gtgctgacac
     2461 aggagggcat cgacgtgtat aacgccatca tcggcggctt cgtgaccgag agcggcgaga
     2521 agatcaaggg cctgaacgag tacatcaacc tgtataatca gaaaaccaag cagaagctgc
     2581 ctaagtttaa gccactgtat aagcaggtgc tgagcgatcg ggagtctctg agcttctacg
     2641 gcgagggcta tacatccgat gaggaggtgc tggaggtgtt tagaaacacc ctgaacaaga
     2701 acagcgagat cttcagctcc atcaagaagc tggagaagct gttcaagaat tttgacgagt
     2761 actctagcgc cggcatcttt gtgaagaacg gccccgccat cagcacaatc tccaaggata
     2821 tcttcggcga gtggaacgtg atccgggaca agtggaatgc cgagtatgac gatatccacc
     2881 tgaagaagaa ggccgtggtg accgagaagt acgaggacga tcggagaaag tccttcaaga
     2941 agatcggctc cttttctctg gagcagctgc aggagtacgc cgacgccgat ctgtctgtgg
     3001 tggagaagct gaaggagatc atcatccaga aggtggatga gatctacaag gtgtatggct
     3061 cctctgagaa gctgttcgac gccgattttg tgctggagaa gagcctgaag aagaacgacg
     3121 ccgtggtggc catcatgaag gacctgctgg attctgtgaa gagcttcgag aattacatca
     3181 aggccttctt tggcgagggc aaggagacaa acagggacga gtccttctat ggcgattttg
     3241 tgctggccta cgacatcctg ctgaaggtgg accacatcta cgatgccatc cgcaattatg
     3301 tgacccagaa gccctactct aaggataagt tcaagctgta ttttcagaac cctcagttca
     3361 tgggcggctg ggacaaggat aaggagacag actatcgggc caccatcctg agatacggct
     3421 ccaagtacta tctggccatc atggataaga agtacgccaa gtgcctgcag aagatcgaca
     3481 aggacgatgt gaacggcaat tacgagaaga tcaactataa gctgctgccc ggccctaata
     3541 agatgctgcc aaaggtgttc ttttctaaga agtggatggc ctactataac cccagcgagg
     3601 acatccagaa gatctacaag aatggcacat tcaagaaggg cgatatgttt aacctgaatg
     3661 actgtcacaa gctgatcgac ttctttaagg atagcatctc ccggtatcca aagtggtcca
     3721 atgcctacga tttcaacttt tctgagacag agaagtataa ggacatcgcc ggcttttaca
     3781 gagaggtgga ggagcagggc tataaggtga gcttcgagtc tgccagcaag aaggaggtgg
     3841 ataagctggt ggaggagggc aagctgtata tgttccagat ctataacaag gacttttccg
     3901 ataagtctca cggcacaccc aatctgcaca ccatgtactt caagctgctg tttgacgaga
     3961 acaatcacgg acagatcagg ctgagcggag gagcagagct gttcatgagg cgcgcctccc
     4021 tgaagaagga ggagctggtg gtgcacccag ccaactcccc tatcgccaac aagaatccag
     4081 ataatcccaa gaaaaccaca accctgtcct acgacgtgta taaggataag aggttttctg
     4141 aggaccagta cgagctgcac atcccaatcg ccatcaataa gtgccccaag aacatcttca
     4201 agatcaatac agaggtgcgc gtgctgctga agcacgacga taacccctat gtgatcggca
     4261 tcgccagggg cgagcgcaat ctgctgtata tcgtggtggt ggacggcaag ggcaacatcg
     4321 tggagcagta ttccctgaac gagatcatca acaacttcaa cggcatcagg atcaagacag
     4381 attaccactc tctgctggac aagaaggaga aggagaggtt cgaggcccgc cagaactgga
     4441 cctccatcga gaatatcaag gagctgaagg ccggctatat ctctcaggtg gtgcacaaga
     4501 tctgcgagct ggtggagaag tacgatgccg tgatcgccct ggaggacctg aactctggct
     4561 ttaagaatag ccgcgtgaag gtggagaagc aggtgtatca gaagttcgag aagatgctga
     4621 tcgataagct gaactacatg gtggacaaga agtctaatcc ttgtgcaaca ggcggcgccc
     4681 tgaagggcta tcagatcacc aataagttcg agagctttaa gtccatgtct acccagaacg
     4741 gcttcatctt ttacatccct gcctggctga catccaagat cgatccatct accggctttg
     4801 tgaacctgct gaaaaccaag tataccagca tcgccgattc caagaagttc atcagctcct
     4861 ttgacaggat catgtacgtg cccgaggagg atctgttcga gtttgccctg gactataaga
     4921 acttctctcg cacagacgcc gattacatca agaagtggaa gctgtactcc tacggcaacc
     4981 ggatcagaat cttccggaat cctaagaaga acaacgtgtt cgactgggag gaggtgtgcc
     5041 tgaccagcgc ctataaggag ctgttcaaca agtacggcat caattatcag cagggcgata
     5101 tcagagccct gctgtgcgag cagtccgaca aggccttcta ctctagcttt atggccctga
     5161 tgagcctgat gctgcagatg cggaacagca tcacaggccg caccgacgtg gattttctga
     5221 tcagccctgt gaagaactcc gacggcatct tctacgatag ccggaactat gaggcccagg
     5281 agaatgccat cctgccaaag aacgccgacg ccaatggcgc ctataacatc gccagaaagg
     5341 tgctgtgggc catcggccag ttcaagaagg ccgaggacga gaagctggat aaggtgaaga
     5401 tcgccatctc taacaaggag tggctggagt acgcccagac cagcgtgaag cacaaaaggc
     5461 cggcggccac gaaaaaggcc ggccaggcaa aaaagaaaaa gggatcctac ccatacgatg
     5521 ttccagatta cgcttatccc tacgacgtgc ctgattatgc atacccatat gatgtccccg
     5581 actatgcctc gagcgactac aaagaccatg acggtgatta taaagatcat gacatcgatt
     5641 acaaggatga cgatgacaag gctgcaggag gcggtggaag cgggagggga gtgcaggtgg
     5701 aaaccatctc cccaggagac gggcgcacct tccccaagcg cggccagacc tgcgtggtgc
     5761 actacaccgg gatgcttgaa gatggaaaga aatttgattc ctcccgggac agaaacaagc
     5821 cctttaagtt tatgctaggc aagcaggagg tgatccgagg ctgggaagaa ggggttgccc
     5881 agatgagtgt gggtcagaga gccaaactga ctatatctcc agattatgcc tatggtgcca
     5941 ctgggcaccc aggcatcatc ccaccacatg ccactctcgt cttcgatgtg gagcttctaa
     6001 aactggaagg atctggtgga agcgggaggg gagtgcaggt ggaaaccatc tccccaggag
     6061 acgggcgcac cttccccaag cgcggccaga cctgcgtggt gcactacacc gggatgcttg
     6121 aagatggaaa gaaatttgat tcctcccggg acagaaacaa gccctttaag tttatgctag
     6181 gcaagcagga ggtgatccga ggctgggaag aaggggttgc ccagatgagt gtgggtcaga
     6241 gagccaaact gactatatct ccagattatg cctatggtgc cactgggcac ccaggcatca
     6301 tcccaccaca tgccactctc gtcttcgatg tggagcttct aaaactggaa ggttctaggg
     6361 gagtgcaggt ggaaaccatc tccccaggag acgggcgcac cttccccaag cgcggccaga
     6421 cctgcgtggt gcactacacc gggatgcttg aagatggaaa gaaatttgat tcctcccggg
     6481 acagaaacaa gccctttaag tttatgctag gcaagcagga ggtgatccga ggctgggaag
     6541 aaggggttgc ccagatgagt gtgggtcaga gagccaaact gactatatct ccagattatg
     6601 cctatggtgc cactgggcac ccaggcatca tcccaccaca tgccactctc gtcttcgatg
     6661 tggagcttct aaaactggaa ggataagcgg ccgcactcct caggtgcagg ctgcctatca
     6721 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc
     6781 ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata
     6841 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     6901 gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc
     6961 aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta
     7021 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta
     7081 gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt
     7141 acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc
     7201 tcttctctta tggagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc
     7261 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     7321 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     7381 cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc
     7441 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     7501 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     7561 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     7621 aaagctaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     7681 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     7741 gtatcttatc atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     7801 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     7861 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     7921 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     7981 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     8041 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     8101 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     8161 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     8221 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     8281 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     8341 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     8401 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     8461 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     8521 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     8581 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     8641 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     8701 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     8761 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     8821 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     8881 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     8941 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     9001 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     9061 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     9121 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     9181 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     9241 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     9301 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     9361 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     9421 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     9481 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     9541 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     9601 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     9661 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     9721 cgcgcacatt tccccgaaaa gtgccacctg
//
