LOCUS       sequence-207314-        9444 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Lb crRNA and human codon optimized LbCpf1(RVR mutant) in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9444)
  AUTHORS   Toth E, Czene BC, Kulcsar PI, Krausz SL, Talas A, Nyeste A, Varga E,
            Huszar K, Weinhardt N, Ligeti Z, Borsy AE, Fodor E, Welker E
  TITLE     Mb- and FnCpf1 nucleases are active in mammalian cells: activities 
            and PAM preferences of four wild-type Cpf1 nucleases and of their 
            altered PAM specificity variants.
  JOURNAL   Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 
            10.1093/nar/gky815.
  PUBMED    30239882
REFERENCE   2  (bases 1 to 9444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815."
COMMENT     Sequence Label: pTE3330
FEATURES             Location/Qualifiers
     source          1..9444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        complement(214..462)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(272..291)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(442..462)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     enhancer        525..828
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        829..1032
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     983..1003
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1077..1096
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1077..1095
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1143..4826
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMRGWDKDVETDRRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             4827..4874
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4881..4961
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(5035..5052)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5041..5265
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5311..5739
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5398..5417)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5608..5629
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5748..5768)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5753..6082
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5933..6068
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5995..6014
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6149..6943
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6203..6222)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6813..6832
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7117..7238
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7154..7173)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7208..7227
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7287..7303)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7287..7303)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7300..7322)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7311..7327
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7335..7341,7342..7359,7360..7365))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7380..7401
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7518..7535)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7689..8277)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7769..7788)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8448..9239,9240..9308))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9071..9090
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9309..9413)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattaaaaa aggagacggg
      181 atcccgtctc catctacact tagtagaaat tacggtgttt cgtcctttcc acaagatata
      241 taaagccaag aaatcgaaat actttcaagt tacggtaagc atatgatagt ccattttaaa
      301 acataatttt aaaactgcaa actacccaag aaattattac tttctacgtc acgtattttg
      361 tactaatatc tttgtgttta cagtcaaatt aattccaatt atctctctaa cagccttgta
      421 tcgtatatgc aaatatgaag gaatcatggg aaataggccc tcactagtta ttaatagtaa
      481 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      541 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      601 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      661 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
      721 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
      781 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      841 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      901 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      961 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     1021 ataagcagag ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat
     1081 acgactcact atagggagac ccaagctggc tagcgtttaa acttaagctt ggtaccgcca
     1141 ccatgagcaa gctggagaag tttacaaact gctactccct gtctaagacc ctgaggttca
     1201 aggccatccc tgtgggcaag acccaggaga acatcgacaa taagcggctg ctggtggagg
     1261 acgagaagag agccgaggat tataagggcg tgaagaagct gctggatcgc tactatctgt
     1321 cttttatcaa cgacgtgctg cacagcatca agctgaagaa tctgaacaat tacatcagcc
     1381 tgttccggaa gaaaaccaga accgagaagg agaataagga gctggagaac ctggagatca
     1441 atctgcggaa ggagatcgcc aaggccttca agggcaacga gggctacaag tccctgttta
     1501 agaaggatat catcgagaca atcctgccag agttcctgga cgataaggac gagatcgccc
     1561 tggtgaacag cttcaatggc tttaccacag ccttcaccgg cttctttgat aacagagaga
     1621 atatgttttc cgaggaggcc aagagcacat ccatcgcctt caggtgtatc aacgagaatc
     1681 tgacccgcta catctctaat atggacatct tcgagaaggt ggacgccatc tttgataagc
     1741 acgaggtgca ggagatcaag gagaagatcc tgaacagcga ctatgatgtg gaggatttct
     1801 ttgagggcga gttctttaac tttgtgctga cacaggaggg catcgacgtg tataacgcca
     1861 tcatcggcgg cttcgtgacc gagagcggcg agaagatcaa gggcctgaac gagtacatca
     1921 acctgtataa tcagaaaacc aagcagaagc tgcctaagtt taagccactg tataagcagg
     1981 tgctgagcga tcgggagtct ctgagcttct acggcgaggg ctatacatcc gatgaggagg
     2041 tgctggaggt gtttagaaac accctgaaca agaacagcga gatcttcagc tccatcaaga
     2101 agctggagaa gctgttcaag aattttgacg agtactctag cgccggcatc tttgtgaaga
     2161 acggccccgc catcagcaca atctccaagg atatcttcgg cgagtggaac gtgatccggg
     2221 acaagtggaa tgccgagtat gacgatatcc acctgaagaa gaaggccgtg gtgaccgaga
     2281 agtacgagga cgatcggaga aagtccttca agaagatcgg ctccttttct ctggagcagc
     2341 tgcaggagta cgccgacgcc gatctgtctg tggtggagaa gctgaaggag atcatcatcc
     2401 agaaggtgga tgagatctac aaggtgtatg gctcctctga gaagctgttc gacgccgatt
     2461 ttgtgctgga gaagagcctg aagaagaacg acgccgtggt ggccatcatg aaggacctgc
     2521 tggattctgt gaagagcttc gagaattaca tcaaggcctt ctttggcgag ggcaaggaga
     2581 caaacaggga cgagtccttc tatggcgatt ttgtgctggc ctacgacatc ctgctgaagg
     2641 tggaccacat ctacgatgcc atccgcaatt atgtgaccca gaagccctac tctaaggata
     2701 agttcaagct gtattttcag aaccctcagt tcatgagggg ctgggacaag gatgtggaga
     2761 ccgatcggcg ggccaccatc ctgagatacg gctccaagta ctatctggcc atcatggata
     2821 agaagtacgc caagtgcctg cagaagatcg acaaggacga tgtgaacggc aattacgaga
     2881 agatcaacta taagctgctg cccggcccta ataagatgct gccaaaggtg ttcttttcta
     2941 agaagtggat ggcctactat aaccccagcg aggacatcca gaagatctac aagaatggca
     3001 cattcaagaa gggcgatatg tttaacctga atgactgtca caagctgatc gacttcttta
     3061 aggatagcat ctcccggtat ccaaagtggt ccaatgccta cgatttcaac ttttctgaga
     3121 cagagaagta taaggacatc gccggctttt acagagaggt ggaggagcag ggctataagg
     3181 tgagcttcga gtctgccagc aagaaggagg tggataagct ggtggaggag ggcaagctgt
     3241 atatgttcca gatctataac aaggactttt ccgataagtc tcacggcaca cccaatctgc
     3301 acaccatgta cttcaagctg ctgtttgacg agaacaatca cggacagatc aggctgagcg
     3361 gaggagcaga gctgttcatg aggcgcgcct ccctgaagaa ggaggagctg gtggtgcacc
     3421 cagccaactc ccctatcgcc aacaagaatc cagataatcc caagaaaacc acaaccctgt
     3481 cctacgacgt gtataaggat aagaggtttt ctgaggacca gtacgagctg cacatcccaa
     3541 tcgccatcaa taagtgcccc aagaacatct tcaagatcaa tacagaggtg cgcgtgctgc
     3601 tgaagcacga cgataacccc tatgtgatcg gcatcgatag gggcgagcgc aatctgctgt
     3661 atatcgtggt ggtggacggc aagggcaaca tcgtggagca gtattccctg aacgagatca
     3721 tcaacaactt caacggcatc aggatcaaga cagattacca ctctctgctg gacaagaagg
     3781 agaaggagag gttcgaggcc cgccagaact ggacctccat cgagaatatc aaggagctga
     3841 aggccggcta tatctctcag gtggtgcaca agatctgcga gctggtggag aagtacgatg
     3901 ccgtgatcgc cctggaggac ctgaactctg gctttaagaa tagccgcgtg aaggtggaga
     3961 agcaggtgta tcagaagttc gagaagatgc tgatcgataa gctgaactac atggtggaca
     4021 agaagtctaa tccttgtgca acaggcggcg ccctgaaggg ctatcagatc accaataagt
     4081 tcgagagctt taagtccatg tctacccaga acggcttcat cttttacatc cctgcctggc
     4141 tgacatccaa gatcgatcca tctaccggct ttgtgaacct gctgaaaacc aagtatacca
     4201 gcatcgccga ttccaagaag ttcatcagct cctttgacag gatcatgtac gtgcccgagg
     4261 aggatctgtt cgagtttgcc ctggactata agaacttctc tcgcacagac gccgattaca
     4321 tcaagaagtg gaagctgtac tcctacggca accggatcag aatcttccgg aatcctaaga
     4381 agaacaacgt gttcgactgg gaggaggtgt gcctgaccag cgcctataag gagctgttca
     4441 acaagtacgg catcaattat cagcagggcg atatcagagc cctgctgtgc gagcagtccg
     4501 acaaggcctt ctactctagc tttatggccc tgatgagcct gatgctgcag atgcggaaca
     4561 gcatcacagg ccgcaccgac gtggattttc tgatcagccc tgtgaagaac tccgacggca
     4621 tcttctacga tagccggaac tatgaggccc aggagaatgc catcctgcca aagaacgccg
     4681 acgccaatgg cgcctataac atcgccagaa aggtgctgtg ggccatcggc cagttcaaga
     4741 aggccgagga cgagaagctg gataaggtga agatcgccat ctctaacaag gagtggctgg
     4801 agtacgccca gaccagcgtg aagcacaaaa ggccggcggc cacgaaaaag gccggccagg
     4861 caaaaaagaa aaagggatcc tacccatacg atgttccaga ttacgcttat ccctacgacg
     4921 tgcctgatta tgcataccca tatgatgtcc ccgactatgc ctaagaattc tgcagatatc
     4981 cagcacagtg gcggccgctc gagtctagag ggcccgttta aacccgctga tcagcctcga
     5041 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     5101 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     5161 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     5221 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     5281 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     5341 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     5401 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     5461 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     5521 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     5581 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     5641 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     5701 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     5761 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     5821 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     5881 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     5941 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     6001 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     6061 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     6121 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     6181 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     6241 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     6301 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     6361 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     6421 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     6481 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     6541 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     6601 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     6661 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     6721 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     6781 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     6841 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     6901 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     6961 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     7021 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     7081 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     7141 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     7201 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     7261 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     7321 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     7381 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     7441 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7501 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7561 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7621 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7681 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7741 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7801 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7861 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7921 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7981 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8041 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8101 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     8161 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8221 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8281 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8341 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8401 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8461 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8521 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8581 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8641 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8701 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     8761 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     8821 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     8881 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8941 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     9001 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     9061 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     9121 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     9181 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     9241 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     9301 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     9361 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     9421 ccccgaaaag tgccacctga cgtc
//
