LOCUS       sequence-207327-        7824 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7824)
  AUTHORS   Beraud C, Sun SC, Ganchi P, Ballard DW, Greene WC
  TITLE     Human T-cell leukemia virus type I Tax associates with and is 
            negatively regulated by the NF-kappa B2 p100 gene product: 
            implications for viral latency.
  JOURNAL   Mol Cell Biol. 1994 Feb . 14(2):1374-82.
  PUBMED    8289813
REFERENCE   2  (bases 1 to 7824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1994 Feb . 14(2):1374-82."
COMMENT     Sequence Label: pCMV4-p105
FEATURES             Location/Qualifiers
     source          1..7824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        178..557
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        558..761
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     712..732
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(4051..4070)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(4498..4518)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4503..4832
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4683..4818
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4871..4967
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     complement(5043..5062)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5044..5062)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5076..5092)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5076..5092)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5089..5111)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5100..5116
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5124..5130,5131..5148,5149..5154))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5169..5190
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5307..5324)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5478..6066)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5558..5577)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6237..7028,7029..7097))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6860..6879
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7098..7202)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7229..7684
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7316..7335)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7526..7547
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     join(7810..7824,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(7824,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgcc aagctgatct atacattgaa tcaatattgg caattagcca
       61 tattagtcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt
      121 atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttgac
      181 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      241 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      301 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      361 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      421 tgtatcatat gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      481 attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag
      541 tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt
      601 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      661 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg
      721 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
      781 attgttttta tttttaattt tctttcaaat acttccatcg aattcagatc tgcttaggag
      841 ggagagccca cccgcgccag gaggccgaac gcggactcgc cacccggctt cagaatggca
      901 gaagatgatc catatttggg aaggcctgaa caaatgtttc atttggatcc ttctttgact
      961 catacaatat ttaatccaga agtatttcaa ccacagatgg cactgccaac agcagatggc
     1021 ccataccttc aaatattaga gcaacctaaa cagagaggat ttcgtttccg ttatgtatgt
     1081 gaaggcccat cccatggtgg actacctggt gcctctagtg aaaagaacaa gaagtcttac
     1141 cctcaggtca aaatctgcaa ctatgtggga ccagcaaagg ttattgttca gttggtcaca
     1201 aatggaaaaa atatccacct gcatgcccac agcctggtgg gaaaacactg tgaggatggg
     1261 atctgcactg taactgctgg acccaaggac atggtggtcg gcttcgcaaa cctgggtata
     1321 cttcatgtga caaagaaaaa agtatttgaa acactggaag cacgaatgac agaggcgtgt
     1381 ataaggggct ataatcctgg actcttggtg caccctgacc ttgcctattt gcaagcagaa
     1441 ggtggagggg accggcagct gggagatcgg gaaaaagagc taatccgcca agcagctctg
     1501 cagcagacca aggagatgga cctcagcgtg gtgcggctca tgtttacagc ttttcttccg
     1561 gatagcactg gcagcttcac aaggcgcctg gaacccgtgg tatcagacgc catctatgac
     1621 agtaaagccc ccaatgcatc caacttgaaa attgtaagaa tggacaggac agctggatgt
     1681 gtgactggag gggaggaaat ttatcttctt tgtgacaaag ttcagaaaga tgacatccag
     1741 attcgatttt atgaagagga agaaaatggt ggagtctggg aaggatttgg agatttttcc
     1801 cccacagatg ttcatagaca atttgccatt gtcttcaaaa ctccaaagta taaagatatt
     1861 aatattacaa aaccagcctc tgtgtttgtc cagcttcgga ggaaatctga cttggaaact
     1921 agtgaaccaa aacctttcct ctactatcct gaaatcaaag ataaagaaga agtgcagagg
     1981 aaacgtcaga agctcatgcc caatttttcg gatagtttcg gcggtggtag tggtgccgga
     2041 gctggaggcg gaggcatgtt tggtagtggc ggtggaggag ggggcactgg aagtacaggt
     2101 ccagggtata gcttcccaca ctatggattt cctacttatg gtgggattac tttccatcct
     2161 ggaactacta aatctaatgc tgggatgaag catggaacca tggacactga atctaaaaag
     2221 gaccctgaag gttgtgacaa aagtgatgac aaaaacactg taaacctctt tgggaaagtt
     2281 attgaaacca cagagcaaga tcaggagccc agcgaggcca ccgttgggaa tggtgaggtc
     2341 actctaacgt atgcaacagg aacaaaagaa gagagtgctg gagttcagga taacctcttt
     2401 ctagagaagg ctatgcagct tgcaaagagg catgccaatg cccttttcga ctacgcggtg
     2461 acaggagacg tgaagatgct gctggccgtc cagcgccatc tcactgctgt gcaggatgag
     2521 aatggggaca gtgtcttaca cttagcaatc atccaccttc attctcaact tgtgagggat
     2581 ctactagaag tcacatctgg tttgatttct gatgacatta tcaacatgag aaatgatctg
     2641 taccagacgc ccttgcactt ggcagtgatc actaagcagg aagatgtggt ggaggatttg
     2701 ctgagggctg gggccgacct gagccttctg gaccgcttgg gtaactctgt tttgcaccta
     2761 gctgccaaag aaggacatga taaagttctc agtatcttac tcaagcacaa aaaggcagca
     2821 ctacttcttg accaccccaa cggggacggt ctgaatgcca ttcatctagc catgatgagc
     2881 aatagcctgc catgtttgct gctgctggtg gccgctgggg ctgacgtcaa tgctcaggag
     2941 cagaagtccg ggcgcacagc actgcacctg gctgtggagc acgacaacat ctcattggca
     3001 ggctgcctgc tcctggaggg tgatgcccat gtggacagta ctacctacga tggaaccaca
     3061 cccctgcata tagcagctgg gagagggtcc accaggctgg cagctcttct caaagcagca
     3121 ggagcagatc ccctggtgga gaactttgag cctctctatg acctggatga ctcttgggaa
     3181 aatgcaggag aggatgaagg agttgtgcct ggaaccacgc ctctagatat ggccaccagc
     3241 tggcaggtat ttgacatatt aaatgggaaa ccatatgagc cagagtttac atctgatgat
     3301 ttactagcac aaggagacat gaaacagctg gctgaagatg tgaagctgca gctgtataag
     3361 ttactagaaa ttcctgatcc agacaaaaac tgggctactc tggcgcagaa attaggtctg
     3421 gggatactta ataatgcctt ccggctgagt cctgctcctt ccaaaacact tatggacaac
     3481 tatgaggtct ctgggggtac agtcagagag ctggtggagg ccctgagaca aatgggctac
     3541 accgaagcaa ttgaagtgat ccaggcagcc tccagcccag tgaagaccac ctctcaggcc
     3601 cactcgctgc ctctctcgcc tgcctccaca aggcagcaaa tagacgagct ccgagacagt
     3661 gacagtgtct gcgacagcgg cgtggagaca tccttccgca aactcagctt taccgagtct
     3721 ctgaccagtg gtgcctcact gctaactctc aacaaaatgc cccatgatta tgggcaggaa
     3781 ggacctctag aaggcaaaat ttagaagctt gcatgcctgc aggtcgactc tagaggatcc
     3841 ttaagttaac ttaatggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag
     3901 ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg
     3961 actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag
     4021 ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg
     4081 cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc
     4141 ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt
     4201 ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga
     4261 tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc
     4321 ctgtccttct gattttaaaa taactatacc agcaggagga cgtccagaca cagcataggc
     4381 tacctggcca tgcccaaccg gtgggacatt tgagttgctt gcttggcact gtcctctcat
     4441 gcgttgggtc cactcagtag atgcctgttg aattgggtac gcggccagct tggctgtgga
     4501 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4561 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     4621 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     4681 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     4741 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     4801 gaggcttttt tggaggccta ggcttttgca aaaagctcct cgaggaactg aaaaaccaga
     4861 aagttaactg gtaagtttag tctttttgtc ttttatttca ggtcccggat ccggtggtgg
     4921 tgcaaatcaa agaactgctc ctcagtggat gttgccttta cttctaggcc tgtacggaag
     4981 tgttacttct gctctaaaag ctgctgcagg tcgactctag ggccgcgtac cgagctcgaa
     5041 ttccctatag tgagtcgtat taaattcgta atcatgtcat agctgtttcc tgtgtgaaat
     5101 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     5161 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     5221 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     5281 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     5341 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     5401 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     5461 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     5521 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     5581 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     5641 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     5701 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     5761 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     5821 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     5881 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     5941 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     6001 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     6061 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     6121 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     6181 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     6241 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     6301 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     6361 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     6421 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     6481 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     6541 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     6601 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     6661 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     6721 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     6781 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     6841 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     6901 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     6961 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     7021 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     7081 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     7141 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     7201 cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg
     7261 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     7321 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     7381 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     7441 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     7501 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     7561 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     7621 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     7681 atttccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     7741 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     7801 cagggttttc ccagtcacga cgtt
//
