LOCUS       sequence-20733-e         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG305GPD-ccdB-TAP
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(130..254)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             313..390
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             418..438
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ggtctcgtta tcggggaaaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
       61 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
      121 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
      181 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
      241 ttgtacaaag tggtgatggg ctgcaggaat tcgatatcaa gcttatcgat aggtggacca
      301 ggtggtggaa tgaagcgacg atggaaaaag aatttcatag ccgtctcagc agccaaccgc
      361 tttaagaaaa tctcatcctc cggggcactt gattatggta ccccaactac tgcttctgaa
      421 aatctatatt ttcaaggtga actaaaaact gctgctttgg ctcaacatgc gattaagggt
      481 gaagctcaaa aacttaatga ctctcaagct ccaaaagctg atgcgcaaca aaataacttc
      541 aacaaagatc aacaaagcgc cttctatgaa atcttgaaca tgcctaactt aaacgaagcg
      601 caacgtaacg gcttcattca aagtcttaaa gacgacccaa gccaaagcac taacgtttta
      661 ggtgaagcta aaaaattaaa cgaatctcaa gcaccgaaag ctgataacaa ttt
//
