LOCUS       sequence-207333-       12724 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of mouse cDNA of FTCD, refractory to the guides in 
            plasmids 1,2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12724)
  AUTHORS   Kanarek N, Keys HR, Cantor JR, Lewis CA, Chan SH, Kunchok T, 
            Abu-Remaileh M, Freinkman E, Schweitzer LD, Sabatini DM
  TITLE     Histidine catabolism is a major determinant of methotrexate 
            sensitivity.
  JOURNAL   Nature. 2018 Jul 11. pii: 10.1038/s41586-018-0316-7. doi: 
            10.1038/s41586-018-0316-7.
  PUBMED    29995852
REFERENCE   2  (bases 1 to 12724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2018 Jul 11. pii: 10.1038/s41586-018-0316-7. doi: 
            10.1038/s41586-018-0316-7."
COMMENT     Sequence Label: pWP1_mFTCD
FEATURES             Location/Qualifiers
     source          1..12724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2120..3298
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2351..3289
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3246..3266
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3346..3463
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    5158..5725
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5176..5727
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5320..5337)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5547..5566
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5730..5732,5733..5735,5736..6446)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5730..5732,5733..5735,5736..6446)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5775..5796)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6036..6055)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6383..6404
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6525..6541)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6526..6542)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    6555..7143
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6608..6628)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7026..7037)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7287..7320)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7340..7520
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7545..7563)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7546..7563)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7853..7872)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8086..8105)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8205..8227
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8265..8283)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8351..8455
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8456..8524,8525..9316)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8674..8693)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9487..10075
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9976..9995
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10229..10246
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10316..10336)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10321..10650
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10501..10636
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11784..11849
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11979..11999
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12424..12558
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12461..12480)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12515..12534
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacgratc tcgacsgtat cgatgtcgac gataagcttt
     2041 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     2101 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     2161 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     2221 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     2281 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     2341 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     2401 cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct
     2461 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
     2521 tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct
     2581 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
     2641 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
     2701 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
     2761 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
     2821 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
     2881 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
     2941 agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
     3001 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
     3061 gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg
     3121 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
     3181 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
     3241 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagg
     3301 aatttcgaca tttaaattta attaatctcg acggtatcgg ttaactttta aaagaaaagg
     3361 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     3421 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttatcgatc acgagactag
     3481 cctcgaggtt taaacctcga ggccaccatg tctcagttgg tggaatgtgt ccccaacttc
     3541 tcagagggga acaaccagga ggtgattgat gccatctctc gagccatctc ccagacccca
     3601 ggctgtgtgc tgttggacgt cgatgccgga ccctccacca accgcactgt ctacactttc
     3661 gtggggcagc cagagtgtgt cgtagagggg gccctccatg ctgcgcgtac ggcctcacag
     3721 ctcatcgaca tgagcaagca caagggtgag caccctcgaa tgggcgctct ggatgtgtgt
     3781 cccttcatcc cggtgagggg cgtcagcatg gaagagtgtg tgctctgtgc caaggcattt
     3841 gggcagcggc tggcagagga acttaatgta ccagtgtatc tctatggtga ggcagctcag
     3901 acgcccagtc gtcagaccct gccagccatc cgggctggag agtatgaggc cctgcctgag
     3961 aagctcaaac aggccgagtg ggtgccagac tttggcccta gctcctttgt ccccagttgg
     4021 ggtgctactg tcacgggtgc acggaagttc ctcattgcat tcaatatcaa cctgctaagc
     4081 accaaggagc aggcccaccg tattgctctt aacctgcggg agcagggccg agggaaagac
     4141 cagcctggac gtctgaaaaa ggtccagggc atcggttggt acctggaaga gaagaacctg
     4201 gctcaggtgt ccacaaacct cctggacttt gaggtcacag ctctgcacac agtgtttgag
     4261 gaggcccgca gagaggctca ggagctgaat ctcccagtgg tgggctcgca gctggtgggc
     4321 ctggtgcctc tgaaggcctt actggatgcc gcagccttct actgtgacaa ggagaagctg
     4381 tttgttctgg aggaggagca tcggatacgg ctggtggtga accggctggg cctggattcg
     4441 ctggcaccct tcgacccaaa ggaacggatc atcgagtacc tagtccccga tagtggacca
     4501 gaacagagcc tgttggacac ctcactgcgc ggttttgttc gggaggtggg agcccgctct
     4561 gctgccccag gaggaggttc tgtagcagca gcggtagcag ctctgggtgc agccctggct
     4621 tctatggtgg gccagatgac ctatggccgg cgccagtttg atcacttgga ttccacaatg
     4681 cgacgcctga tcccaccctt ccacgcagcc tctgcccagc tgacttcatt ggtggatgct
     4741 gatgcccggg cgtttgctgc ctgtttggag gcaataaagc tgcccaagaa cacgcctgaa
     4801 gagagagaca ggcgtgcatg cgctctgcag gaggggctga gacaggcagt cgctgtgcca
     4861 ctgaaactgg ccgagactgt gtcccagcta tggccagcgc tgcaggagct ggcccactgt
     4921 gggaacctgt cctgcttgtc tgacctgcag gtggcagcca aagccttgga gacaggtgtg
     4981 tttggtgcgt acttcaacgt gctcatcaac ctgaaggaca tgacggatga tgtatttaaa
     5041 gagaagacac accatcgaat ctccagcctt ctgcaggaag ccaagactca ggctgcgctg
     5101 gtgctgggta gcctagaagc ccgaaaggag tgagtttaaa ctacgggctg caggaattcc
     5161 gccccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     5221 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     5281 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     5341 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     5401 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     5461 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     5521 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     5581 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     5641 ttacatgtgt ttagtcgagg ttaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     5701 tttcctttga aaaacacgat gataatacca tggtgagcaa gggcgaggag ctgttcaccg
     5761 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     5821 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     5881 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     5941 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     6001 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     6061 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     6121 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     6181 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     6241 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     6301 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     6361 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     6421 ctctcggcat ggacgagctg tacaagtccg gactcagatc tcgactagct agtagctagc
     6481 tagctagtcg agctcaagct tcgaattcga tatcaagctt atcgcgatac cgtcgacctc
     6541 gagggaattc cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     6601 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     6661 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     6721 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     6781 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     6841 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     6901 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     6961 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     7021 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     7081 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     7141 cgcatcggga attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat
     7201 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     7261 caagatggga tcaattcacc atgggaataa cttcgtatag catacattat acgaagttat
     7321 gctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7381 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7441 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7501 agtgtggaaa atctctagca gcatctagaa ttaattccgt gtattctata gtgtcaccta
     7561 aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc taacgacaat
     7621 atgtacaagc ctaattgtgt agcatctggc ttactgaagc agaccctatc atctctctcg
     7681 taaactgccg tcagagtcgg tttggttgga cgaaccttct gagtttctgg taacgccgtc
     7741 ccgcacccgg aaatggtcag cgaaccaatc agcagggtca tcgctagcca gatcctctac
     7801 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
     7861 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
     7921 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
     7981 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
     8041 atgcaggagt cgcataaggg agagcgtcga atggtgcact ctcagtacaa tctgctctga
     8101 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     8161 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     8221 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     8281 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     8341 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     8401 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     8461 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     8521 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     8581 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     8641 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     8701 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     8761 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     8821 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     8881 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     8941 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     9001 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     9061 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     9121 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     9181 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     9241 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     9301 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     9361 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     9421 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     9481 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     9541 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     9601 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     9661 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     9721 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     9781 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     9841 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     9901 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     9961 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
    10021 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
    10081 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
    10141 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
    10201 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
    10261 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctgtggaat
    10321 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
    10381 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
    10441 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
    10501 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
    10561 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
    10621 ggcttttttg gaggcctagg cttttgcaaa aagcttggac acaagacagg cttgcgagat
    10681 atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga
    10741 ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc aacctatttt
    10801 cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca tgagcgaaaa
    10861 atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa gccgactgat
    10921 gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgt accgggtgcg
    10981 ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat
    11041 cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc
    11101 atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat
    11161 ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgac
    11221 tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat gggcgtcgta
    11281 ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt
    11341 ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg ctgcatccat
    11401 gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc
    11461 gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg
    11521 taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggcat
    11581 tgacccacgc gaaatcctcg acgtccaggc acgtattgtg atgagcgatg ccgaacgtac
    11641 cgacgatgat ttatacgata cggtgattgg ctaccgtggc ggcaactgga tttatgagtg
    11701 ggccccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac
    11761 ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac
    11821 tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc
    11881 agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg
    11941 atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag
    12001 aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta
    12061 atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca
    12121 agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata
    12181 acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc
    12241 aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt
    12301 atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct
    12361 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
    12421 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
    12481 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
    12541 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
    12601 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
    12661 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
    12721 gtag
//
